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Clinical Application of Cryopreserved Allograft Valves and Establishment of Cryopreserved Tissue Bank

Research Project

Project/Area Number 04454349
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Thoracic surgery
Research InstitutionNara Medical College

Principal Investigator

KITAMURA Soichiro  Nara Medical College, Department of Surgery III,Professor, 医学部, 教授 (10028607)

Co-Investigator(Kenkyū-buntansha) KAWATA Tetsuji  Nara Medical College, Department of Surgery III,Clinical Instructor, 医学部, 助手 (60204731)
FUKUTOMI Masaaki  Nara Medical College, Department of Surgery III,Clinical Instructor, 医学部, 助手 (40221535)
TANIGUCHI Shigeki  Nara Medical College, Department of Surgery III,Clinical Instructor, 医学部, 助手 (90183467)
KAWACHI Kanji  Nara Medical College, Department of Surgery III,Associate Professor, 医学部, 助教授 (90116020)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1992: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsAllograft valve / Cryopreservation / Tissue bank / Flowcytometry / Cell viability
Research Abstract

Human allograft valves have increasingly been used as a semilunar valve substitute with the advent of cryopreservation techniques in many developed countries except Japan. We introduced the tissue cryopreservation system for the purpose of clinical application of allograft valves in our hospital. Allograft valves were procured from the cadavers and donors of renal transplantation with a consent. Allograft valves were collected under the sterilized manner and were cryopreserved after sterilization with antibiotic solution for 24 hours. The cryopreserved allograft valves were frozen by a program freezer in the nutrient medium containing 10% dimethylsulfoxide, and subsequently stored in liquid nitrogen (-196゚C). For the clinical use of the allograft valve, we assessed bacterial growth and cell viability of the allograft valve before storage, after antibiotic sterilization and at 30 days of cryopreservation. We evaluated cell viability of the allograft valve by flowcytometry, that revealed that fibroblasts of the cryopreserved allograft valve were well preserved (over 70%) if warm ischemic time was less than 520 minutes (8.7 hours). Following germ-free confirmation and cell viability assessment, cryopreserved aortic allograft valves were implanted in 18 patients having aortic valve disease with good results. In our clinical investigation, the aortic allograft valve is an ideal substitute for the semilunar valve from the exercise hemodynamic aspect, particulary in patients with a small aortic root. Cryopreserved pulmonary allograft valves harvested from the adult donor were also successfully implanted as a size-reduced pulmonary allograft for an RV-PA conduit in children with pulmonary atresia.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] 北村惣一郎、庭屋和夫、他: "新鮮(fresh)あるいは凍結保存(cryopreserved)同種大動脈弁と用いた大動脈弁置換手術。" 日本胸部外科学会会誌. 40. 2035-2039 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 庭屋和夫、北村惣一郎、他: "ヒト同種弁の冷蔵及び凍結保存におけるcell viabilityの研究。" 日本胸部外科学会会誌. 41. 1335-1340 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 北村惣一郎、庭屋和夫、他: "Homograft (Allograft) valve手術の現状。" 臨床胸部外科. 14. 273-279 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Soichiro Kitamura、et al.: "Size-reduced cryopreservev pulmonary vaive allograft for an RV-PA conduit : Technical modification and functional evaluation." J Card Surg. 10. 14-20 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kazuo Niwaya、et al.: "The effect of warm ischemia and cryopreservation on cell viability of human allograft valves." Ann Thorac Surg.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 長谷川順一、北村惣一郎、他: "同種大動脈弁による大動脈弁置換術後の運動負荷時弁機能の検討-Bicer弁及びSt.Jude Medical(SJM)弁との比較。" 日本胸部外科学会会誌. (掲載予定). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 北村惣一郎、庭屋和夫: "最新胸部外科手術「Homograftによる大動脈弁置換術」日本胸部外科学会卒後教育委員会" 日本胸部外科学会卒後教育委員会, 13 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 北村惣一郎、庭屋和夫: "Annual Review循環器1994「凍結保存同種生体弁移植」" 中外医学社, 6 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Soichiro KITAMURA,Kazuo NIWAYA,Kanji KAWACHI,Ryuichi MORITA,Shigeki TANIGUCHI,Tetsuji KAWATA,Shuichi KOBAYASHI,Masaaki FUKUTOMI,Youichi KAMEDA,Junichi HASEGAWA: "Aortic valve replacement with fresh or cryopreserved aortic allograft-Initial experience in Japan-" J Jpn Assn Thorac Surg. 40. 2035-2039 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kazuo NIWAYA,Soichiro KITAMURA,Kanji KAWACHI,Ryuichi MORITA,Shigeki TANIGUCHI,Toshio SEKI,Masaaki FUKUTOMI,Tetsuji KAWATA: "Cell viability assessment of cold-preserved and cryopreserved allograft valves by flowcytometric analysis" J Jpn Assn Thorac Surg. 41. 1335-1340 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Soichiro KITAMURA,Kanji KAWACHI,Kazuo NIWAYA,Masaaki FUKUTOMI,Shuichi KOBAYASHI,Junichi HASEGAWA,Tetsuji KAWATA,Hidehito SAKAGUCHI: "Size-reduced cryopreservedpulmonary valve allograft for an RV-PA conduit : Technical modification and functional evaluation" J Card Surg. 10. 14-20 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kazuo NIWAYA,Kanji KAWACHI,Hidehito SAKAGUCHI,Soichiro KITAMURA: "The effect of warm ischemia and cryopreservation on cell viability of human allograft valves" Ann Thorac Surg. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 北村惣一郎、庭屋和夫: "ヒト同種弁を用いる大動脈弁置換手術。" 医学のあゆみ. 159. 923-923 (1991)

    • Related Report
      1994 Annual Research Report
  • [Publications] 北村惣一郎、庭屋和夫他: "新鮮(fresh)あるいは凍結保存(cryopreserved)同種大動脈弁を用いた大動脈弁置換手術。" 日本胸部外科学会会誌. 40. 2035-2039 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] 庭屋和夫、北村惣一郎他: "ヒト同種弁の冷蔵及び凍結保存におけるcell viabilityの研究。" 日本胸部外科学会会誌. 41. 1335-1340 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 北村惣一郎、庭屋和夫他: "Homograft(Allograft)valve手術の現状。" 臨床胸部外科. 14. 273-279 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 北村惣一郎、川田哲嗣他: "総特集、人工臓器の進歩「人工弁」。" 先端医療. 3. 39-41 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Soichiro Kitamura、et al.: "Size-reduced cryopreservev pulmonary valve allograft for an RV-PA conduit:Technical modification and functional evaluation." J Card Surg. 10. 14-20 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 北村惣一郎、庭屋和夫: "最新胸部外科手術「Homograftによる大動脈弁置換術」" 日本胸部外科学会卒後教育委員会, 13 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 北村惣一郎、庭屋和夫: "Annual Review循環器1994「凍結保存同種生体弁移植」" 中外医学社, 6 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 庭屋和夫、他: "ヒト同種弁保存における温阻血時間のcell viabilityにおよぼす影響" 日本外科学会雑誌. 95. 74- (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 庭屋和夫、他: "ヒト同種弁の冷蔵及び凍結保存におけるcell viabilityの研究" 日本胸部外科学会雑誌. 41. 1335-1340 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 庭屋和夫、他: "ヒト同種弁の冷蔵および凍結保存におけるcell viabilityの検討-flowcytometry法を用いて-." 日本胸部外科学会雑誌. 40. 1583- (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 北村惣一郎、他: "新鮮(fresh)あるいは凍結保存(cryopreserved)同種大動脈弁を用いた大動脈弁置換手術" 日本胸部外科学会雑誌. 40. 2035-2039 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 北村惣一郎、他: "ヒト同種弁を用いる大動脈弁置換手術" 医学のあゆみ. 159. 923- (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 庭屋和夫、他: "抗生物質加nutreit medium内保存のfresh homograft valveの細菌学的、組織学的検討." 日本胸部外科学会雑誌. 39. 1405- (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 北村惣一郎、他: "「Homograftによる大動脈弁置換術.」日本胸部外科学会第35回卒後教育セミナーテキスト" 日本胸部外科学会, 13 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 北村惣一郎、他: "凍結保存同種生体弁移植 Annual Review 循環器1994" 中外医学社, 6 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 北村 惣一郎、他: "新鮮(fresh)あるいは凍結保存(cryopreserved)同種大動脈弁を用いた大動脈弁置換手術" 日本胸部外科学会雑誌. 40. 2035-2039 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 庭屋 和夫、他: "ヒト同種弁の冷蔵および凍結保存におけるcell viabilityの検討ーflowcytometry法を用いてー" 日本胸部外科学会雑誌. 40. 1583- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 庭屋 和夫、他: "抗生物質加nutreit medium内保存のfresh homograft valveの細菌学的、組織学的検討." 日本胸部外科学会雑誌. 39. 1405- (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 北村 惣一郎、他: "ヒト同種弁を用いる大動脈弁置換手術" 医学のあゆみ. 159. 923- (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 庭屋 和夫、他: "Flowcytometry法を用いた保存ヒト同種弁のcell viabilityの検討ー短期氷温保存の可能性" 日本心臓血管外科学会雑誌. 22(Supple). 145- (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 北村 惣一郎、庭屋 和夫: "日本胸部外科学会第35回卒後教育セミナーテキスト「Homograftによる大動脈弁置換術.」" 日本胸部外科学会, 13 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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