| Project/Area Number |
04454349
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | Nara Medical College |
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Principal Investigator |
KITAMURA Soichiro Nara Medical College, Department of Surgery III,Professor, 医学部, 教授 (10028607)
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| Co-Investigator(Kenkyū-buntansha) |
KAWATA Tetsuji Nara Medical College, Department of Surgery III,Clinical Instructor, 医学部, 助手 (60204731)
FUKUTOMI Masaaki Nara Medical College, Department of Surgery III,Clinical Instructor, 医学部, 助手 (40221535)
TANIGUCHI Shigeki Nara Medical College, Department of Surgery III,Clinical Instructor, 医学部, 助手 (90183467)
KAWACHI Kanji Nara Medical College, Department of Surgery III,Associate Professor, 医学部, 助教授 (90116020)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1992: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | Allograft valve / Cryopreservation / Tissue bank / Flowcytometry / Cell viability |
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| Research Abstract |
Human allograft valves have increasingly been used as a semilunar valve substitute with the advent of cryopreservation techniques in many developed countries except Japan. We introduced the tissue cryopreservation system for the purpose of clinical application of allograft valves in our hospital. Allograft valves were procured from the cadavers and donors of renal transplantation with a consent. Allograft valves were collected under the sterilized manner and were cryopreserved after sterilization with antibiotic solution for 24 hours. The cryopreserved allograft valves were frozen by a program freezer in the nutrient medium containing 10% dimethylsulfoxide, and subsequently stored in liquid nitrogen (-196ﾟC). For the clinical use of the allograft valve, we assessed bacterial growth and cell viability of the allograft valve before storage, after antibiotic sterilization and at 30 days of cryopreservation. We evaluated cell viability of the allograft valve by flowcytometry, that revealed that fibroblasts of the cryopreserved allograft valve were well preserved (over 70%) if warm ischemic time was less than 520 minutes (8.7 hours). Following germ-free confirmation and cell viability assessment, cryopreserved aortic allograft valves were implanted in 18 patients having aortic valve disease with good results. In our clinical investigation, the aortic allograft valve is an ideal substitute for the semilunar valve from the exercise hemodynamic aspect, particulary in patients with a small aortic root. Cryopreserved pulmonary allograft valves harvested from the adult donor were also successfully implanted as a size-reduced pulmonary allograft for an RV-PA conduit in children with pulmonary atresia.
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