| Project/Area Number |
04454358
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Nagoya University |
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Principal Investigator |
YOSHIDA Jun Nagoya University School of Medicine Assistant Prof., 医学部, 講師 (40158449)
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| Co-Investigator(Kenkyū-buntansha) |
YAGI Kunio Institute of Applied Biochemistry President, 所長 (00022749)
SEO Hisao Nagoya University Institute of Enviornmental Medicine Professor, 環境医学研究所, 教授 (40135380)
WAKABAYASHI Toshihiko Nagoya University School of Medicine Instructor, 医学部, 助手 (50220835)
SUGITA Kenichiro Nagoya University, School of Medicine Professor, 医学部, 教授 (30023807)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥4,700,000 (Direct Cost: ¥4,700,000)
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| Keywords | gene therapy / brain tumor / interferon / liposome / monoclonal antibody / モノクローナル抗体 |
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| Research Abstract |
Our approach developing gene therapy to malignant glioma is selectively transfer of cytokine gene into the tumor cells by means of DNA/immunoliposomes (monoclonal antibody coupled cationic liposomes). We confim in the present research work, the anti-tumor effect of transfection-induced HuIFN-beta by the use of human glioma produced in the brain of nude mice. We injected humanglioma cells into the cerebral hemisphere. The human gliomas grew in the mouse brain and they all made a huge brain tumor. For the evaluation of anti-tumor effect, we injected liposomes with entrapped pSV2IFN-beta into the tumor growing tn the brain once every second day for six times. We started the treatment at different days after the transplantation and sacrified all animals at 31st day, and it has been demonstrated that the transplanted glioma disappasred completely when the liposomes with entrapped pSV2IFN-beta was injected repeatedly into the tumor. The complete disappearance of the transplanted glioma was seen in 100% of nude mice if the treatment was started until 3 days after transplantation and when it was started at 7 days or 9 days, the tumor-free ratio was 5/7 (71%) or 2/7 (29%) respectively. Futhermore, the anti-tumor effect of transfection-induced HuIFN-beta was noted to be increased by using DNA/immunoliposomes.
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