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Research on generation mechanism and in vitro diagnosis of malignant hyperthermia

Research Project

Project/Area Number 04454396
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 麻酔学
Research InstitutionToho University

Principal Investigator

KIKUCHI Hirosato  Toho University School of Medicine, Professor, 医学部, 教授 (40034029)

Co-Investigator(Kenkyū-buntansha) SATA Ken  Toho University School of Medicine, Assistant, 医学部, 助手 (00235300)
MAEDA Noriko  Toho University School of Medicine, Assistant, 医学部, 助手 (30173355)
SHINODA Takahide  Toho University School of Medicine, Assistant, 医学部, 助手 (50175387)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsmalignant hyperthermia / skinned fiber technique / intramuscular calcium ion(Ca^<2+>) metabolism / Ca^<2+>-induced Ca^<2+>-release(CICR) / in vitro diagnosis / neuromuscular diseases / denervated skeletal muscle / Ca^<2+> channel blockers / 筋小胞体からのカルシウム誘発性カルシウム遊離速度(Calcium-induced Calcium release rete, CICR rate) / スキンド・ファイバー法 / ミオグロビン尿症 / 高CK血症 / カフェイン感受性試験 / カルシウム拮抗薬
Research Abstract

It is generally accepted that malignant hyperthermia, one of the serious complications of general anesthesia, results from abnormality of calcium ion(Ca^<2+>) metabolism in skeletal muscle. The skinned fiber technique which is essentially taken as an experimental method for this study can independently examine the components of Ca^<2+> metabolim in skeletal muscle (1.Ca^<2+>-induced Ca^<2+> release rate from sarcoplasmic reticulum, CICR rate. 2.Ca^<2+> uptake by sarcoplasmic reticulum. 3.Ca^<2+> sensitivity of contractile system proteins), and is applied to in vitro diagnosis of malignant hyperthermia. In the present study, we have accumulated the data of human skeletal muscles biopsied from the patients who were clinically suspected of the above disease, their family members and the patients who clinically presented malignant hyperthermia-like manifestations (myogloblinuria, hyper creatininekinasemia, etc) and tried to answer some of clinical questions. Also intra-muscular Ca^<2+> metabolism in some of neuro-muscular diseases was examined using the skinned fiber method, and one specific abnormality in one certain disease was identified. With animal specimens (guinea pig), denervated muscle presented lowered sensitivity of contraction to certain drugs (caffeine, one of xanthine delivertives and halothane, one of volatile anesthetics) based on the change in intramuscular Ca^<2+> metabolism, and this result may explain uncertainty of the caffeine, halothane contracture test which is used in North American and European countries for in vitro diagnosis of malignant hyperthermia. Again with animal skinned fibers the effect of some of the clinically used drugs (Ca^<2+> channel blockers, etc) on intramuscular Ca^<2+> metabolism were examined and their clinical safety was discussed.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 坂田治人,塚原純雄,菊地博達ほか: "悪性高熱症が疑われた一例" 麻酔と蘇生(悪性高熱症の進歩). 28(別冊27). 65-69 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 前原康弘,向田圭子,久保田稔ほか: "わが国の悪性高熱症の集計(1991年)" 麻酔と蘇生(悪性高熱症の進歩). 28(別冊27). 79-83 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 松井一幸,内田寿美子,長谷川愼一ほか: "悪性高熱症の診断-スキンドファイバー法を中心に-" 麻酔と蘇生(悪性高熱症の進歩). 28(別冊27). 85-88 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 内田寿美子,長谷川愼一,松井一幸ほか: "高CK血症と悪性高熱症" 麻酔と蘇生(悪性高熱症の進歩). 29(別冊29). 11-16 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 内田寿美子,長谷川愼一,松井一幸ほか: "東邦大学における筋生検テストの集計" 麻酔と蘇生(悪性高熱症の進歩). 29(別冊29). 17-20 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 長谷川愼一,内田寿美子,松井一幸ほか: "除神経後の骨格筋のカフェイン感受性" 麻酔と蘇生(悪性高熱症の進歩). 29(別冊29). 25-28 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sakata H, Tsukahara S, Kikuchi H, et al.: "An Abortive Case of Malignant Hyperthermia" Anesthesia and Resuscitation. 28. 65-69 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Maehara Y, Mukaida K, Kubota M, et al.: "Statistical Review of Malignant Hyperthermia in Japan(1991)" Anesthesia and Resuscitation. 28. 79-83 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsui K, Uchida S, Hasegawa S, et al.: "The Problems in Diagnosis of Malignant Hyperthermia in Japan" Anesthesia and Resuscitation. 28. 85-88 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Uchida S, Hasegawa S, Matsui K, et al.: "Hyper-CKemia and Malignant Hyperthermia" Anesthesia and Resuscitation. 29. 11-16 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Uchida S, Hasegawa S, Matsui K, et al.: "Statistical Review of Skinned Fiber Test is Toho University" Anesthesia and Resuscitation. 29. 17-20 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hasegawa S, Uchida S, Matsui K, et al.: "Sensitivity of the Denervated Skeletal Muscle Fibers to Caffeine" Anesthesia and Resuscitation. 29. 25-28 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 坂田治人、塚原純雄、菊地博達: "悪性高熱症が疑われた一例" 麻酔と蘇生(悪性高熱症の進歩). 28(別冊27). 65-69 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 前原康弘、向田圭子、久保田稔ほか: "わが国の悪性高熱症の集計(1991年)" 麻酔と蘇生(悪性高熱症の進歩). 28(別冊27). 79-83 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 松井一幸、内田寿美子、長谷川慎一ほか: "悪性高熱症の診断-スキンドファイバー法を中心に-" 麻酔と蘇生(悪性高熱症の進歩). 28(別27). 85-88 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 内田寿美子、長谷川慎一、松井一幸ほか: "高CK血症と悪性高熱症" 麻酔と蘇生(悪性高熱症の進歩). 29(別冊29). 11-16 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 内田寿美子、長谷川慎一、松井一幸ほか: "東邦大学における筋生検テストの集計" 麻酔と蘇生(悪性高熱症の進歩). 29(別冊29). 17-20 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 長谷川慎一、内田寿美子、松井一幸ほか: "除神経後の骨格筋のカフェイン感受性" 麻酔と蘇生(悪性高熱症の進歩). 29(別冊29). 25-28 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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