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The role of extracellular matrix in growth and differentiation of prostatic cells

Research Project

Project/Area Number 04454404
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionKagawa Medical School

Principal Investigator

WADA Fumio  Kagawa Medical School, Department of Endocrinology, Professor, 医学部, 教授 (20028385)

Co-Investigator(Kenkyū-buntansha) OYA Haruyo  Kagawa Medical School, Department of Endocrinology, Research Associate, 医学部, 助手 (80223973)
NISHI Nozomu  Kagawa Medical School, Department of Endocrinology, Research Associate, 医学部, 助手 (10145047)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1993: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1992: ¥2,800,000 (Direct Cost: ¥2,800,000)
KeywordsProstate / Epithelial-Stromal Ineractions / Growth Factor / Primary Culture / Extracellular matrix / Androgen / 上皮ー間質間相互作用
Research Abstract

The effect of extracellular matrix (ECM) on the proliferation and differentiation of primary cultured rat prostate cells was examined. The results for the proliferation were as follows : Among the ECM components tested, only laminin stimulated growth of the epithelial cells. Fibronectin and type IV collagen showed growth-inhibiting effects on the epithelial cells, and the effects were partly neutralized by the presence of laminin. On the other hand, growth of the stromal cells was not affected by these ECM proteins, but attachment of the cells to culture dishes was significantly stimulated by treating the dishes with fibronectin before use. Matrigel, a solubilized basement membrane preparation, stimulated growth of the epithelial cells. However, the addition of Matrigel directly to the culture medium resulted in the same effect as that induced by coating the culture dishes with Matrigel, which indicated that a soluble factor(s) may participate in the growth-promoting effect of Matrigel.
The effect of ECM on the differentiated function (production of secretory proteins) of prostate epithlial cells was not examined, as we could not detect the production of secretory proteins under all the conditions tested. The search for the culture conditions under which the epithelial cells express the finally differentiated function is now under progress.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Nishi Nozomu: "An anti-probasin monoclonal antibody recognizes a novel 40-kDa protein localized in rat liver and a specific region of kidney urinary tubule." Biochim.Biophys.Acta.1117. 47-54 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yasuda Yoshiko: "Induction of avascular yolk sac due to reduction of basic fibroblast growth factor by retinoic acid in mice." Dev.Biol.150. 397-413 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nishi Nozomu: "Identification of probasin-related antigen as cystathionine γ-lyase by molecular cloning." J.Biol.Chem.269. 1015-1019 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nishi Nozomu: "An anti-probasin monoclonal antibody recognizes a nocel 40-kDa protein localized in rat liver and a specific region of kidney urinary tubule." Biochim.Biophys.Acta.1117. 47-54 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yasuda Yoshiko: "Induction of avascular yolk sac due to reduction of basic fibroblast growth factor by retinoic acid in mice." Bev.Biol.150. 397-413 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nishi Nozomu: "Identification of probasin-related antigen as cystathionine gamma-lyase by molecular cloning." J.Biol.Chem.269. 1015-1019 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nishi Nozomu: "Identification of probasin-related antigen as cystathionine γ-lyase by molecular cloning." J.Biol.Chem.269. 1015-1019 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nishi N.: "An anti-probasin monoclonal antibody recognizes a novel 40-kDaprotein localized in rat liver and a specific region of kidney urinary tubule." Biochim.Biophys.Acta.1117. 47-54 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yasuda Y.: "Induction of avascular yolk sac due to reduction of basic fibroblast growth factor by retinoic acid in mice." Dev.Biol.150. 397-413 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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