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Surgical Rehabilitation of Ocular Surface : Epithelial Transplantation

Research Project

Project/Area Number 04454442
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Ophthalmology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

KINOSHITA Shigeru  Kyoto Pref Univ Med, Ophth, Prof, 医学部, 教授 (30116024)

Co-Investigator(Kenkyū-buntansha) OHASHI Yuichi  Ehime University, Ophth, Prof, 医学部, 教授 (00116005)
NISHIDA Kohji  Kyoto Pref Univ Med, Ophth, Assist, 医学部, 助手 (40244610)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1992: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsCorneal epithelium / Cell culture / Immortalization / Conjunctival epithelium / Epithelial transplantation / KGF / TGF-beta / 増植制御因子 / 株化
Research Abstract

We aimed at developing effective treatment for primary ocular surface disorders. For this, in this project, we mainly focused on the methods controlling proliferation and differentiation of corneal epithelial cells. First, we established a noble cell culture system for freeze and long-term subculture of rabbit corneal epithelial cells. This culture media is composed of very low Ca^<2+> concentration with high aminoacid contents. Second, we established an immortalized rabbit corneal epithelial cell line. For this, primary cultured rabbit corneal epithelial cells were infected with a recombinant SV40-adenovirus vector and were cloned. This cell line shares properties consistent with normal corneal epithelial cells. Although these cells were not transferable as a epithelial transplantation at this point, our success has made a marked progress in handling corneal epithelial cells in vitro. Third, we investigated biological roles of several cell growth and/or regulatory factors. For instance, epidermal growth factor, which mainly comes from lacrimal gland, promoted corneal epithelial cell growth. Similar phenomenon was found in keratinocyte growth factor, but this was produced only in the corneal stromal cells. On the other hand, transforming growth factor -beta did inhibit corneal epithelial cell growth. We also studied spartial distribution of three TGF-beta isoforms in the cornea, and found TGF- beta 2 predominantly.
In the study of epithelial allograft rejection in vivo, we are now investigating IL-2 levels in tears in patients with cyclosporin treatment after corneal epithelial transplantation.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Torishima H: "Basal medium specially designed for culturing rabbir corneal epithelial cells" Investigative Ophthalmology & Visual Science. 33. 1240 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kinoshita S: "Clinical application of human epidermal growth factor in ocular surface disorders" Journal of Dermatology. 19. 680-683 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Araki K: "Immortalization of rabbit corneal epithelial cells by a recombinant SV 40-adenovirus vector" Investigative Ophthalmology & Visual Science. 34. 2665-2671 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 西田幸二: "Transforming growth factor-βによる器官培養角膜上皮の創傷治癒遅延に関する研究" 日本眼科学会雑誌. 97. 899-905 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sotozono C: "Effect of keratinocyte growth factor on corneal epithelial cell proliferation and KGF mRNA expression in the cornea" Investigative Ophthalmology & Visual Science. 34. 1301 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nishida K: "Immunohistochemical localization of transforming growth factor-β1,-β2,-β3 in human cornea" Investigative Ophthalmology & Visual Science. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 木下 茂,大橋 裕一,崎元 卓: "角膜疾患への外科的アプローチ" メジカルビュー社, 199 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 木下 茂: "眼表面疾患の診療" 文光堂, 237 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Torishima H Kinoshita S et al: "Basal medium specially designed for culturing rabbit corneal epithelial cells" Invest Ophth Vis Sci. 33. 1240 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kinoshita S: "Clinical application of human epidermal growth factor in ocular surface disorders" J Dermtol. 19. 680-683 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Araki K Kinoshita S et al: "Immortalization of rabbit corneal epithelial cells by a recombinant SV 40-adenovirus vector" Invest Ophth Vis Sci. 34. 2665-2671 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nishida K Kinoshita S et al: "Transforming growth factor beta inhibits epithelial wound healing of organ-cultured rabbit cornea" J Jpn Ophthalmol Soc. 97. 899-905 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sotozono C Kinoshita S et al: "Effect of keratinocyte growth factor on corneal epithelial cell proliferation and KGF mRNA expression in the cornea" Invest Ophth Vis Sci. 34. 1301 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nishida K Kinoshita S et al: "Immunohistochemical localization of transforming growth factor -beta1, -beta2, -beta3 in human cornea" Invest Ophth Vis Sci. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Araki kaoru: "Immortalization of rabbit cornenal epithelial cells by a recombinant SV 40-adenovirus vector" Investigative Ophthalmology & Visual Science. 34. 2665-2671 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 西田幸二: "Transtorming growth factor-betaによる器官培養角膜上皮の創傷治癒遅延に関する研究" 日本眼科学会雑誌. 97. 899-905 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nishida Kohji: "Immunohistochemical localization of transforming growth factor-beta1,-beta2,-beta3in human cornea" Investigative Ophdthalmology & Visual Science. (印刷中). (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 木下 茂: "眼表面疾患の診療" 文光堂, 237 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Torishima H,Kinoshita S Nezu E et al: "Basal medium specially designed for culturing rabbit corneal epithelial cells" Invect Ophthalmol Vis Sci. 33S. 1240 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Araki K,Ohash Y,Sasabe et al: "Characterization of newly established rabbit corneal epithelial all line" Invest Ophthalmol Vis Sci. 33S. 1240- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 木下 茂,大橋 裕一,崎元 卓: "角膜疾患への外科的アプローチ" メジカルビュー社, 199 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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