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Mechanisms of induction of actoantibodies by oral mycoplasemas

Research Project

Project/Area Number 04454450
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionHokkaido University

Principal Investigator

WWATANABE Tsuguo  Hokkaido University School of Dentistry Professor, 歯学部, 教授 (10064362)

Co-Investigator(Kenkyū-buntansha) INOUE Satoshi  Hokkaido University School of Dentistry Instructor, 歯学部, 助手 (80184745)
SHIBATA Ken-ichiro  Hokkaido University School of Dentistry Instructor, 歯学部, 助手 (50145265)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1993: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥5,100,000 (Direct Cost: ¥5,100,000)
KeywordsMycoplasma salivarium / Antibody to cholesterol / Expression of MHC class II atigen / IgG Fc binding protein / IgG Fc binding activity / Autoantibody / Raji cell / マイコプラズマ / 細胞膜タンパク質 / IgG Fc レセプター / IgG:Fc結合活性 / MHC class II抗原 / Mycoplasma orale / コレステロール / ヤルジオライピン / 膜タンパク質 / IgG Fc
Research Abstract

Mycoplasmas were isolated from 95%, 67% and 71% of samples of saliva, epithelial cells from gingiva and vuccal mucosa, respectively. Rations of numbers of strains identifed as M.salivarium to those as M.orale in strains isolated from saliva samples, cells from gingiva and buccal mucosa of subjects with oral infections were 4 : 1, 3 : 1 and 1 : 4, respectively. Thus, M.salivarium was shown to be more prevalent than any other oral mycoplasmas.
IgG antibodies to both M.salivarium and cholesterol were detected in 18 subjects with oral infections. There was a significant, positive correlation (r=4256, p<0.05) between antibody titers to the organism and those to cholesterol.
Expression of MHC class II antigen (HLA-DR) in Raji cells was stimulated by incubation of the cells with heat-killed M.salvarium cells.
M.salivarium cells bound IgG from humans and animals wia the Fc part. The organism cells, treated with pronase, did not exhibit the IgG Fc-binding activity, and SDS-PAGE showed that cell slurface proteins (88, 90 and 150 kDa) were digested. Therefore, these poteins were considered to be responsible for the activity. A 90 kDa protein (90kp) was shown to be a major progein by western immunoblotting, and purifed.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] 渡邉継男: "Binding of Fc fragments of IgG from human and seven animal species to Mycoplasma salivarium." Microbios. 76. 77-84 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 沢禎彦: "ヒトIgG Fc断片と結合するMycoplasma salivariumの膜タンパク質について" 第20回日本マイコプラズマ学会記録集. 23-26 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 沢禎彦: "Purification and characterization of a membrane protein(90Kp)from Mycoplasma salivarium,which binds IgG Fc fragments" Microbiology and Immunology. 38(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Watanabe, Tsuguo: "Binding of Fc fragments of IgG from human and seven animal species to Mycoplasma salivarium." Microbios. 76. 77-84 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sawa, Yoshihiko: "Cell membrane porteins of Mycoplasma salivarium, which bind Fc fragments of human IgG." Proceedings of 20th congress of Japanese Society of Mycplasmology. 23-26 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sawa, Yoshihiko: "Purification and characterization of a membrane protein (90kp) from Mycoplasma salivarium, which binds immunoglobulin (Ig) G Fc fragments." Microbiology. 38 : in press. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 渡邊継男: "Binding of Fc fragments of IgG from human and seven animal species to Mycoplasma salivarium cells." Microbios. 76. 77-84 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 沢 禎彦: "ヒトIgGのFc部位と結合するMycoplasma salivariumの細胞膜タンパク質について" 第20回日本マイコプラズマ学会記録. 23-26 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 沢 禎彦: "Purification and characterization of a membrane protein (90 KDa) from Mycoplasma salivarium,which binds immunoglobulin (Ig)G Fc fragment" Microbiology and Immunology. 38(印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yoshihiko Sawa: "Jmmunoglobulin G Fc fragmeut-binding proteins in Mycoplasma salivarium cells" Microbiology and Jmmunology. 36. 655-659 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 沢 禎彦: "ヒトIgGのFc部位と結合するMycoplasma salivariumの細胞膜タンパクについて" 第19回日本マイコプラズマ学会記録. 79-82 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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