| Project/Area Number |
04454475
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Conservative dentistry
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
KAWAGOE Masataka Kagoshima Univ., Dental Sch., Professor, 歯学部, 教授 (40028741)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ARIMURA Yuko Kagoshima Univ., Dental Sch., Research Associate, 歯学部, 助手 (30222764)
IWAMOTO Yuko Kagoshima Univ., Dental Hospital, Research Associate, 歯学部附属病院, 助手 (10191454)
NAGAOKA Shigetaka Kagoshima Univ., Dental Sch., Associate Professor, 歯学部, 助教授 (10155913)
TAKADA Haruhiko Kagoshima Univ., Dental Sch., Professor, 歯学部, 教授 (30135743)
|
|---|
| Project Period (FY) |
1992 – 1993
|
| Project Status |
Completed (Fiscal Year 1993)
|
| Budget Amount *help |
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1993: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥5,100,000 (Direct Cost: ¥5,100,000)
|
|---|
| Keywords | Oral anaerbers / TNF / IL-6 |
|---|
| Research Abstract |
In this study, we studied the induction ability of inflammatory cytokines, tumor necrosis factor a (TNF-a) and interleukin (IL)-6, by whole cells of some oral Bacteroides species in human peripheral blood mononuclear cell cultures. The TNF-a and IL-6 activity in culture supernatants were determined by measuring the cytotoxic activity against L929 cells and the in vitro groth of the IL-6 dependent cell line, MH60.BSF2, respectively. Blood samples were collected from five patients, who were clinically diagnosed as having severe periapical pathosis, and five adult healthy volunteers. As a result, the whole cells of Prevotella intermedia, Porphyromonas asaccharolytics and Prevotella melaninogenica induced TNF-a and IL-6 activities in the patient specimens of periapical pathosis.
|
