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Analysis of the mechanism of lymph node metastasis of oral carcinomas

Research Project

Project/Area Number 04454497
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 外科・放射線系歯学
Research InstitutionNiigata University

Principal Investigator

NAKAJIMA Tamio  School of Dentistry Professor, 歯学部, 教授 (10014010)

Co-Investigator(Kenkyū-buntansha) HAMAMOTO Yoshioki  School of Dentistry Assistant Professor, 歯学部, 助手 (40231526)
NOMURA Tsutomu  Dental Hospital Assistant Professor, 歯学部・附属病院, 助手 (20228365)
SHINGAKI Susumu  School of Dentistry Associate Professor, 歯学部, 助教授 (30134943)
SAKU Takashi  School of Dentistry Professor, 歯学部, 教授 (40145264)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1992: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsOral carcinoma / Lymph node metastasis / Basement membrane / Lymphatic invasion / Flow cytometry / リンパ管浸襲 / 間質反応
Research Abstract

The mechanism of lymph node metastasis of oral carcinomas was studied using human oral carcinomas and an animal model of squamous cell carcinoma with a high metastatic potential to lymph nodes in hamsters.
Flow cytometric analysis of 90 fresh and 96 paraffin-embedded tissue samples of oral carcinomas showed that although the incidence of aneuploidy in primary tumors with metastasis was higher than those without metastasis, there was na evident tendency to a shift down to diploidy at metastatic lymph nodes. The results indicate that the chance of evolution of metastatic cell lines is higher in aneuploid tumors than in diploid tumors, possibly because the former are more heterogenous ; however, most cell lines responsible for causing lymph node metastasis are diploid.
An analysis of basement membrane formation around tumor nests of 0-1N during the process of lymph node metastasis showed that with the decrease of tumor nest size, the disruption of basement membrane became conspicuous, in particular ato the invasion front. The findings suggest that the disappearance of besement membrane associated with reduction in size of tumor nests is an essential sign for local invasion of tumor cells leading to lymphatic invasion and metastasis to regional lymph nodes. The growth of the tumor was accompanied by a proliferation of abundant dilated lymphatic vessels which contained clusters of tumor cells. On serial sections, these clusters were in continuity with adjacent tumor nests, indicating that the key step of lymph node metastasis is direct invasion of lymphatic vessels by tumors cells which may differ from the way of hematogenous metastasis.
Stromal reactions studied in human oral carcinomas showed a similarity between primary and metastatic sites, indicaintg that the tumor has its own potency to induce its specifec stroma.
The results of this research were reported in international journals and meetings.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Chen RB,Suzuki K,Nomura T,Nakijima T: "Flow cytometric analysis of squamous cell carcinomas of oral cavity in relation to lymph node metastasis." J Oral Maxillofac Surg. 51. 397-401 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohtake K,Shingaki S,Nakajima T: "Histologic study on the metastatic process in the experimental model of lymph node metastasis." Oral Surg. 75. 472-478 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakajima T: "Flow cytometric analysis of squamous cell carcinoma of tongue-Discussion-" J Oral Maxillofac Surg. 52. 578-579 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Suzuki K,Chen RB,Nomura T,Nakajima T: "Flow cytometric Analysis of primary and metastatic squamous cell carcinomas of the oral and maxillofacial region." J Oral Maxillofac Surg. 52. 855-861 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shingaki S,Nomura T,Kobayashi T,Nakajima T: "Induction chemotherapy with methotrexate and bleomycin in squamous cell carcinoma of the head and neck." Asian J Oral Maxillofac Surg. 6. 41-47 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshizawa M,Shingaki S,Nakajima T,Saku T: "Histopathological study on lymphatic invasion in squamous cell carcinoma(O-1N)with high potential of lymph node metastasis." Clin Exp Metastasis. 12. 347-356 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Chen RB,Suzuki K,Nomura T,Nakajima T: "Flow cytometric analysis of squamous cell carcinomas of the oral cavity in relation to lymph node metastasis" J Oral Maxillofac Surg.51. 397-401 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohtake K.Shingaki S.Nakajima T: "Histologic study on the metastatic process in the experimental model of lymph node metastasis" Oral Surg.75. 472-478 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mahmood JU,Nomura T,Suzuki K,Che RB,Nakajima T: "Flow cytometric analysis of head and neck carcinomas in relation to lymph node metastasis" Oral Oncology ed.by AK Varma et al.Macmillan. Vol.III. 459-462 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakajima T: "Flow cytometric analysis of squamous cell carcinoma of the tongue-Discussion-" J Oral Maxillofac Surg.52. 578-579 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Suzuki K,Chen RB,Nomura T,Nakajima T: "Flow cytometric analysis of primary and metastatic squamous cell carcinomas of the oral and maxillofacial region" J Oral Maxillofac Surg.52. 855-861 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shingaki S,Nomura T,Kobayashi T,Suzuki I,Kohno M,Mahmood JU,Nakajima T: "Induction chemotherapy with methotrexate and bleomycin in squamous cell carcinoma of the head and neck" Asian J Oral Maxillofac Surg.6. 41-47 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshizawa M,Shingaki S,Nakajima T,Saku T: "Histopathological study on lymphatic invasion in squamous cell carcinoma (0-1N) with high potential of lymh node metastasis" Clin Exp Metastasis. 12. 347-356 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kobayashi Y,Nakajima T,Saku T: "Loss of basement membranes in the invading front of 0-1N,hamster squamous cell carcinoma with high lymph carcinoma with high lymph node metastatic potential : an immunohistochemical study for laminin and type IV collagen" Path Internat. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takada M,Fukuda T,Shingaki S,Nakajima T: "Establishment of a cell line derived from squamous cell carcinoma with a high potency to regional lymph nodes" Asian J Oral Maxillofac Surg.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Chen RB,Suzuki K,Nomura T,Nakijima T: "Flow cytometric analysis of squamous cell carcinomas of oral cavity in relation to lymph node metastasis." J Oral Maxillofac Surg. 51. 397-401 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohtake,Shingaki S;Nakajima T: "Histologic study on the metastatic process in the experimental model of lymph node metastasis." Oral Surg. 75. 472-478 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohtake,Shingaki S;Nakajima T: "Histologic study on the metastatic process in the experimental model of lymph node metastasis." Oral Surg. 75. 472-478 (1993)Nakajima T:

    • Related Report
      1994 Annual Research Report
  • [Publications] "Flow cytometric analysis of squamous cell carcinoma of tongue‐Discussion‐" J Oral Maxillofac Surg. 52. 578-579 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Suzuki K,Chen RB,Nomura T,Nakajima T: "Flow cytometric Analysis of primary and metastatic squamous cell carcinomas of the oral and maxillofacial region." J Oral Maxillofac Surg. 52. 855-861 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shingaki S,Nomura T,Kobayashi T,Nakajima T: "Induction chemotherapy with methotrexate and bleomycin in squamous cell carcinoma of the head and neck." Asian J Oral Maxillofac Surg. 6. 41-47 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yoshizawa M,Shingaki S,Nakajima T,Saku T: "Histopathological study on lymphaticinvasion in squamous cell carcinoma(O‐1N)with high potential of lymph node metastasis." Clin Exp Metastasis. 12. 347-356 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] R.B.Chen,K.Suzuki,T.Nomura,T.Nakajima: "Flow cytometric analysis of squamous cell carcinomas of the oral cavity in relation to lymph node metastasis" J.Oral.Maxillo fac.Surg.51. 397-401 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Ohtake,S.Shingaki,T.Nakajima: "Histologic study on the metastatic process in the experimental model of lymph node metastatsis" Oral Surg.75. 472-478 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] J.U.Mahmood,T.Nomura,K.Suzuki,R.B.Chen,T.Nakajima: "Flow cytometric analysis of head and neck carcinomas in relation to lymph node metastasis" Oral Oncology. 3. 459-462 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] R.B.Chen,K.Suzuki T.Nomura,T.Nakajima: "Flow cytometric analysis of squamous cell carcinoma of the oral cavity in relation to lymph node metastasis." J.Oral Maxillofac,Surg.51. (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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