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Activation and regulation of macrophages by Cryo-destructed tumor cells

Research Project

Project/Area Number 04454499
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 外科・放射線系歯学
Research InstitutionOsaka University

Principal Investigator

SAKUDA Masayoshi  Osaka University, Faculty of Dentistry Professor, 歯学部, 教授 (00028755)

Co-Investigator(Kenkyū-buntansha) MORIYAMA Tomoji  Osaka University, Hospital attached to Faculty of Dentistry Senior resident, 歯学部・附属病院, 医員
SUMI Totsuro  Osaka University, Faculty of Dentistry Research assistant, 歯学部, 助手 (40252697)
NAKAZAWA Mitsuhiro  Osaka University, Hospital attached to Faculty of Dentistry Research assistant, 歯学部・附属病院, 助手 (70217701)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1993: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1992: ¥3,500,000 (Direct Cost: ¥3,500,000)
KeywordsCryosurgery / Macrophage / Immunological regulation / Interferon / Tumor immunity / 細胞性免疫 / サイトカイン
Research Abstract

We investigate the immunological responses, especially about macrophages elicited by cryo-destructed Meth A(Cryo-MA) or Mitomycin C-treated Meth A(MMC-MA) as a model experiment of cryosurgery against malignant tumor.
Spleen cells of Cryo-MA inoculated mice showed antitumor activity in Winn assay, but higher activity was observed in MMC-MA inoculated mice. The effecter cells induced by Cryo-MA were macrophages and natural killer(NK) cells, although MMC-MA increased the activity of macrophases and cytotoxic T lymphocytes (CTL). The appearance of macrophages' cytostatic activity was delayd in Cryo-MA arm compared with MMC-MA arm.
Macrophages of Cryo-MA arm enhanced the producing activity of interleukin-1, alpha-interferon(IFN) and prostaglandin E2. Macrophages of MMC-MA arm enhanced the production of TNF and increased Ia antigen positive ratio. These findings suggest that macrophages of both groups are activated different immunological mechanisms. It was found that Cryo-MA playd a role as an alpha-interferon inducer, and the macrophages stimulated with Cryo-MA produced alpha-IFN in vitro. Intraperitoneal administration of the anti-alpha-IFN antibody carried down-regulation of macrophage cytostatic and NK activity of Cryo-MA inoculated mice, whereas MMC-MA mice were not. CTL activity enhanced some extent by administration of anti-alpha-IFN antibody. These facts suggest that the alpha-IFN have two ways of immunological regulation, the first one is the activation of cytostatic activity of macrophage and NK activity, and the other is down-regulation of CTL activity by suppression of Ia antigen expression of macrophages.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Tetsuro Sumi: "Suppression of tumorigenecity in temperature-sensitive mutants of embryonal carcinoma cells by induction of cell differentiation at non-permissive temperature." Int.J.Cancer. 54. 333-337 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 森山知是: "下顎歯肉扁平上皮癌に関する臨床統計的検討-後発転移症例の検討-" 頭頚部腫瘍. 19. 207-210 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 瀧田正亮: "口腔扁平上皮癌の組織学的悪性度に関する研究-悪性度スコアと生存率との相関性について-" 大阪大学歯学会雑誌. 36. 359-368 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 中澤光博: "下顎歯肉扁平上皮癌の顎骨吸収様式と予後との関連性" 日本口腔科学会雑誌. 40. 589-599 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Toshiyuki Yoneda: "Frequency of the Hypercalcemia-Leukocytosis Syndrome in Oral Malignancies" Cancer. 168. 617-622 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 作田正義: "カラー・癌手術ここがポイント 舌癌" 癌治療・今日と明日. 13. 25-28 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 作田正義: "顎口腔外科診断治療大系1991(分担執筆)" 講談社, 880 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tetsuro Sumi, et al: "Suppression of tumorigenecity in temperature-sensitive mutants of embryonal carcinoma cells by induction of cell differentiation at non-permissive temperrature" Int.J.Cancer. 54. 333-337 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Souichi Iwai, et al.: "Changes in Hox.1.6, c-jun, and Oct-3 gene expression are associated with teratocarcinoma F9 cell differentiation in three different ways of induction" Exp.Cell Res.205. 39-43 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tomoji Moriyama, et al.: "Clinicostatistical investigation on squamous cell carcinoma of the lower alveolus and gingiva -Secondary metastasis-" Toukeibushuyou. 19. 207-210 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mitsuhiro Nakazawa, et al.: "The relationship between prognosis and the mode of bone resorptions by carcinomas involving the mandible" J.Jpn.Stomatol.Soc. 40. 589-599 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masaaki Takita.et al.: "Histopathological grading of malignancy in oral squamous cell carcinoma statistically analysis on correlation between malignancy score and survival rate" J.Osaka Univ.Dent.Soc. 36. 359-368 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 森山知是: "下顎歯肉扁平上皮癌に関する臨床統計的検討-後発転移症例の検討-" 頭頚部腫瘍. 19. 207-210 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tetsuro Sumi: "Suppression of tumorigenicity in temperature-sensitive mutants of embryonal carcinoma cells by induction of cell differentiation at non-permissive temperature" Int.J.Cancer. 54. 333-337 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Souichi Iwai: "Changes in Hox1.6,c-jun,and Oct-3 gene expressions are associated with teratocarcinoma F9 cell differentiation in three different ways of induction" Exp.Cell Res.205. 39-43 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tetsuo Ohnishi: "Intracerebroventricular treatment of mice with pertussis toxin induces hyperalgesia and enhances ^3H-nitrendipine binding to synaptic membranes:similarity with morphine tolerance." Naunyn-Schmiedeberg's Arch Pharmacol. 341. 123-127 (1990)

    • Related Report
      1992 Annual Research Report
  • [Publications] Toshiyuki Yoneda: "Three cases of oral squamous cancer associated with leukocytosis,hypercalcemia, or both." Oral Surg. Oral Med. Oral Pathol.68. 604-611 (1989)

    • Related Report
      1992 Annual Research Report
  • [Publications] 作田 正義: "下顎悪性腫瘍の病態とその処置法" 日本口腔外科学会雑誌. 35. 1705-1709 (1989)

    • Related Report
      1992 Annual Research Report
  • [Publications] 中澤 光博: "頭頚部扁平上皮癌の臨床的研究 高齢者と若壮年者の比較検討" 日本口腔外科学会雑誌. 36. 1296-1307 (1990)

    • Related Report
      1992 Annual Research Report
  • [Publications] 森山 知是: "担癌マウスの抗腫瘍効果に与える放射線局所照射の影響 -放射線障害部位での免疫学的検討-" 大阪大学歯学会雑誌. 32. 181-209 (1987)

    • Related Report
      1992 Annual Research Report
  • [Publications] 墨 哲郎: "マウステラトカルシノーマ細胞における腫瘍性増殖と細胞分化に関する研究ー温度感受性変異株の細胞分化と造腫瘍性の変化ー" 大阪大学歯学会雑誌. 32. 210-228 (1987)

    • Related Report
      1992 Annual Research Report
  • [Publications] 作田 正義: "口腔粘膜の病変(分担執筆)" 医歯薬出版, 202 (1990)

    • Related Report
      1992 Annual Research Report
  • [Publications] 作田 正義: "口腔外科学(分担執筆・編集代表 宮崎 正)" 738 (1988)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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