| Project/Area Number |
04454528
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Hokkaido University |
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Principal Investigator |
YASUYUKI Nomura Hokkaido Univ..Fac.Pharm.. Prof., 薬学部, 教授 (00034041)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1993: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1992: ¥5,300,000 (Direct Cost: ¥5,300,000)
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| Keywords | Neuroimmune interaction / Human lymphocyte / Cytokine / Interleukin 2 / NG 108-15 cell / Neurite elongation / Glial cell / Nitric acid dynthase / NG 108-15細胞 / TA-20 / エンドトキシン / 神経細胞死 / 神経伝達物質 / アセチルコリン受容体 / インターロイキン2 / 許容効果 / インターロイキン2受容体 |
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| Research Abstract |
To clarigy functional significances and the transmenbrane signgalling mechanism of neurotransmitter actions in lymphocytes and cytokine actions in neurons, we inverstigated cellular responses, in particular gene expression of physiological active molecules, to acetylcholine in T cells and to LPS in glial cells and to NO in neurons.
We found that : 1) m_3 type of acetylcholine receptors is present in human T cells and the stimulation causes inositol 3-phosphate fromation followed by an increase in [Ca^<2+>]i, 2)m_3 receptors seem to be involved in potentiation of lectin-induced formation of IL-2 and IL-2 receptors.
We also obtained interesting findings that : 1) LPS induces de novo sythesis of nitric oxide synthase (iNOS) in glial cells via 110 kDa proteins phosphorylated by protein tyrosine kinase, 2) NO generated by iNOS in glial cells probably penetrates into surrounding neurons an causes cell death.
We succeeded cloning and sequencing of cDNA of a novel neuronal differentiation factor, TA 20 from NG108-15 cells and found that TA 20 is a protein with molecular size of approx. 20 kDa whose gene expression is activated by treatment with TPA and dibutylyl cAMP.We are further examining functional roles of TA20 and mechanism of the gene espression.
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