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Studies on the mechanism of autoimmune poliglandular endocrine disorders determined ny antipituitary antibodies

Research Project

Project/Area Number 04454545
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionGunma University

Principal Investigator

KOBAYASHI Isao  Gunma Univ.Schol of Med.Dept.Qf Laboratory Medicine, Professor., 医学部, 教授 (50008273)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1993: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1992: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsantipituitary antibodies. / pituitary dwarfism. / isolated ACTH deficiency. / pituitary antibodies. / Graves' disease. (6) Hashimoto's thyroiditis. / 橋本病 / 自己免疫性疾患
Research Abstract

We have established an assay method by western Blot analysis of antipituitary antibodies. and evaluated on clinical significance of determination of antipituitary antibodies.
Antigens was obtained from rat pituitary cytosolic fractions. 50 ul of sample(200 ug protein) was mixed with 50 ul of smple buffer. Searated proteins were transfered to a PVDF membrane by a semi-dry blotting apparatus. The membrane was incubated in 1 : 101 diluted human serum.
We have fund 22 kDa positive band after identification of pituitary antigens recognized by antipituitary antibodies. The prevalence of positive oituitary antibodies by this method was 40.0%(12/30) in patents with insulin-dependent diabetes melltus, while nobody showed positive results in patients withnon-insulin dependent diabetes mellitus. The 22 kDa positive band was found in 14 of 53 patients with Graves' disease(26.4%) and 12 of 54 patients with Hashimoto's thyroditis(22.2%). Eleven of 25 patients with pituitary dwarfism showed positive antibodies(44.0%).
The 22 kDa protein was considered to be GH-related protein in rat pituitary cytosolic fractions.
Thus, we have demonstrated a new assay system by western Blot analysis of antipituitary antibodies. We are now in progress to determine pituitary cell surface membrane antibodies.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] 小林功,他: "下垂体系:特に下垂体抗体をめぐって" 臨症病理. 41. 123-127 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 小林他: "抗下垂体抗体の測定法" 日本臨床. 51. 2673-2678 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Isao Kobayashi,et al.: "80 Years OF HASHIMOTO DISEASE(edited by S.Nagataki et al):Changes i circulating autoantibodies to pituitary and thyroid after delivery." Excerpta Medica,Amsterdam, 3 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Isao Kobayashi, et al.: "Pituitary system : With special reference to pituitary antibodies" Jaanese Journal of Clinical Pathology.Vol.41. 123-127 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Isao Kobayashi, et al.: "Assay method of antipituitary antibodies" Nihon Rinsho. Vol.51. 2673-2678 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Isao Kobayash, et al.: "Changes in circulating autoantibodies to pituitary and thyroid after delivery." 80 Years of Hashimoto Disease.(edited by S.Naataki, et al).Excerpta Medica, Amsterdam.487-489 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 小林功,他: "下垂体系:特に下垂体抗体をめぐって" 臨床病理. 41. 123-127 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 小林他: "抗下垂体抗体の測定法" 日本臨床. 51. 2673-2678 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Isao Kobayashi,et al.: "80 Years OF HASHIMOTO DISEASE(edited by S.Nagataki et al):Changes i circulating autoantibodies to pituitary and thyroid after delivery." Excerpta Medica,Amsterdam, 3 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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