| Project/Area Number |
04454549
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Showa University |
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Principal Investigator |
MAEDA Masako Showa University Associated Professor, 薬学部, 助教授 (00053869)
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| Co-Investigator(Kenkyū-buntansha) |
ARAKAWA Hidetoshi Showa University Assistant Professor, 薬学部, 講師 (70129807)
TSUJI Akio Showa University Professor, 薬学部, 教授 (80053784)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1993: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1992: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | immunoassay / bioluminescece / enzymeimmunoassay / acetate kinase / Iuciferin / Iuciferase / ATP / bioluminescent wnzymeimmunoassay / イムノアッセイ / 生物発光検出法 / ATP / 微量分析法 / 生物発光酵素イムノアッセイ |
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| Research Abstract |
A sensitive bioluminescent enzyme immunoassay (BLEIA) has been developed using acetate kinase (AK) as a new label enzyme.
The bioluminescent assay of AK was done by forefly luciferase for measuring ATP produced by enzymatic reaction of AK using acetylphosphate and ADP as substrate. The detection limit of AK was 60 zmole/assay (1.2 amole/ml) and the coefficient of variation (CV,n=5) for each points, ranged from 2.1 to 8.3%. The proposed bioluminescent assay of AK was very simple, rapid and highly sensitive. The AK assay developed here, was applied to BLEIA of 17alpha hydroxyprogesterone, rabbit IgG,human thyroid stimulating hormone, human corionic gonadtropin and human transferin.
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