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Molecular mechanism and its pathophysiological significance of the cooperation between ATP or adenosine and TSH in thyroid cells

Research Project

Project/Area Number 04454553
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionGunma University

Principal Investigator

KONDO Yoichi  Gunma University, Institute for Mol.and Cell.Regul., Professor, 生体調節研究所, 教授 (70008598)

Co-Investigator(Kenkyū-buntansha) SHO Kimie  Gunma University, Institute for Mol.and Cell.Regul.Research Assistant, 生体調節研究所, 教務員 (40201561)
TOMURA Hideaki  Gunma University, Institute for Mol.and Cell.Regul., Assistant, 生体調節研究所, 助手 (70217553)
OKAZIMA Fumikazu  Gunma University, Institute for Mol.and Cell.Regel., Assistan Professor, 生体調節研究所, 助教授 (30142748)
KONDO Yoichi  Gunma University, Institute for Mol.and Cell.Regul., Professor (70008598)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1993: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1992: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsTSH / Adenosine / Signal Transduction system / Cross talk / Receptor / Gene Transfection / Hydrogen Peroxide / Calsium / ATP / 甲状腺 / Ca^<2+> / FRTL-5細胞 / アラキドン酸生成 / カルモジュリン / 甲状腺胞細 / カルシウムイオン / アラキドン酸 / フラ2 / ホスホリパーゼA2
Research Abstract

In the recent years, through experiments using recombinant TSH receptors, it has been shown that one kind of receptor molecule can activate both of the signal transduction systems, the cAMP system and the phospholipase (PLC) -Ca^<2+> system. On the other hand, we have found that adenosine (Ado) which is derived from ATP can change the flow of the information by affecting the effect of TSH as to enhancing the activation of PLC-Ca^<2+> system, and at thesame time inhibiting the activation of the cAMP system. In this study, such modulation mechanism of Ado on the effect of TSH has been analyzed by means of cellular and molecular biological methods. The results are as follows.
1) Proved that the TSH/Ado cooperation occurs inside one cell : By tracing the change of Ca^<2+> in a FRTL-5 thyroid cell using Digital Video Imaging, we showed that both TSH and Ado affect the same type of Ca^<2+> pools located at various regions inside one cell.
2) Discovery of a new target of the Ado-induced modulat … More in : When the cellular Ca^<2+> concentration was raised without using TSH,this increased Ca^<2+> activated PLA2.Ado enhanced this function of elevated Ca^<2+> as well. Therefore Ado can modulate themetabolic control system in two steps. One before the mobilization of Ca^<2+> and another after it.
3) Reconstruction of the TSH/Ado cooperation mechanism : We prepared a model cell by introducing AdoA_1 receptor cDNA together with either muscarinic M3 receptor cDNA or TSH receptor cDNA.The observed functions of the cells indicated that the enhancement of TSH reaction before and after the Ca^<2+> mobilizatios, and the inhibition of the activation of adenylate-cyclase by TSH,all occurs by kind of A_1 receptor.
4) The identihfication of a PLC subtype participating in the cooperation of TSH and Ado : Ado enhanced the TSH-induced activation of PLCBbeta subtype but did not betaFGF-induced activation of PLCgamma, suggesting that Ado effect is specific to the PLCbeta activation which the G protein participates in.
5) Ado enhances the hydrogen peroxide formation by TSH : TSH induces the formation of hydrogen peroxide in thyroid cells. This TSH effect is brought about through the Ca^<2+> signaling system, and PLA^2 participates in it as well. By enhancing the activations of PLC and A_2 in this signal transduction system, Ado enhances the hydrogen peroxide formation inducing effect of TSH. Less

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (64 results)

All Other

All Publications (64 results)

  • [Publications] T.Kimura: "Thyrotropin-indeuced hydrogen peroxide production in FRTL-5 thyroid cells is mediated not by adenosine 3',5'-monophosphate,but by Ca^<2+> signaling followed by phospholipase A2 activatiion and potentiated by an adenosine derivative." Endocrinology. 136. 116-123 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Sho: "Cooperation of TSH but not basic fibroblast growth factor with an adenosine receptorat gonist in Ca2+ mobilization from thapsigargin sensitive pools in single FRTL-5 thyroid cells." Endocrinology. 136. 770-778 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] F.Okajima: "Intracellular corss-talk between thyrotropin receptor and A1 adenosine receptor inregulation of phospholipase C and adenylate cyclase in COS-7 cells transfected withtheir receptor genes." Biochem.J.306. 709-715 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Shimegi: "Permissive stimulation of Ca2+ induced phospholipase A2 by an adenosine receptoragonist in a pertussis toxin-sensitive manner in FRTL-5 thyroid cells:a new 'crosstalk'mechanism in Ca2+ signalling." Biochem.J.299. 845-851 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Tomura: "Transfected human somatostatin receptor type2, SSTR2, not only inhibits adenylatecyclase but also stimulates phospholipase C and Ca2+ mobilization." BiochemBiophys.Res.Commun. 200. 986-992 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Akbar: "A singlespecies of A1 adenosine receptor oexpressed in chinese hamster ovary cells not only inhibits cAMP accumulation but also stimulates phospholipase C and arachidonaterelease." Mol.Pharmacol.45. 1036-1042 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Shimura: "Thyroid-specific expression and cyclic adenosine 3',5'-monophosphateautoregulation of the thyrotropin receptor gene involves thyroid transcriptionfactor-1." Mol.Endocrinology. 8. 1049-1069 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] F.Okajima: "Genistein, an inhibitor of protein tyrosine kinase, is also a competitive antagonist for P1-purinergic (adenosine)receptor in FRTL-5 thyroid cells." Biochem.Biophys.Res.Commun.203. 1488-1495 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] F.Okajima: "Enkephalinactivates phospholipase C-Ca2+ system through a crown-talk between oplold receptors and P2-purinorgic(xbradykinin receptors in NG 108-15 cells:A permissive role for portummln toxlnsoiixtlvo C1-protein." Biochem.J.290. 241-247 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.A.Majid: "UTP activates phospholipase C-Ca2+ system through a receptor different from the 53-kDn ATP receptorin PC12 cells." Biochem.Biophys.Res.195. 415-421 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] A.Hidaka: "Receptor cross-talk can optimize assays for autoantibodies to the thyrotropin receptor:Effect of phonylisopropyladenosine on adenosine 3',5'-monophosphate and inositolphosphate levels in rat" J.Clin.Endocrinol.Metab.77. 1164-1169 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Shoda: "Graves' immunoglobulin G stimulatesiodide efflux in FRTL-5 thyroid cells." J.Clin.Endocrinol.Metab.77. 94-97 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Kosugi: "Substitutions of different regions of the thrid cytoplasmic loop of the thyrotropin(TSH) receptor have selective effects on constirutive, TSH-,and TSH receptor auto antibody-stimulated." Mol.Endocrinol.7. 1009-1020 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Sato: "Extracellular ATP stimulates three different receptor signal transduction systems in FRTL-5 thyroid cells." Biochem.J.283. 281-287 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] F.Okajima: "Synergism in Cytosolic Ca2+ mobilization between bradykinin and agonists forpertussis toxin-sensitive G-protein-coupled receptors in NG 108-15 cells." FEBS Lett.301. 223-226 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Tomura: "Discrimination between two types of P2 purinoceptors by suramin inrat hepatocytes." Eur.J.Pharmacol.226. 363-365 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.A.Majid: "Characterication of ATP receptor which mediates norepinephrine release in PC12 cells." Biochimica et Biophysica Acta. 1136. 283-289 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Tomura: "Enkephalin induces Ca2+ monobilization in single cells of bradykininsensitized differentiated neuroblastoma hybridoma (NG108-15)cells." Neurosci.Lett.148. 93-96 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Tada: "P2 purinoceptor-mediated cyclic AMP accumulation in bovine vascular smooth muscle cells." Eur.J.Pharmacol.227. 25-31 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Kosugi: "Mutation of alanine 623 in the third cytoplasmic loop of the rat thyrotoropin (TSH) receptor results in a loss in the phosphoinositide but not cAMP signal induced by TSH and receptor auto antibodies." J.Biol.Chem.267. 24153-24156 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.kimura: "Thyrotropin-induced hydrogen peroxide production in FRTL-5 thyroid cells is mediated not by adenosine 3', 5'-monophspate, but by Ca^<2+> signaling followed by phospholipase A2 activation and potentiated by an adenosine derivative" Endocrinology. 136. 116-123 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Sho: "Cooperation of TSH but not basic fibroblast growth factor with an adenosine receptor agonist in Ca^<2+> mobilization from thapsigargin sensitive pools in single FRTL-5 thyroid cells" Endocrinology. 136. 770-778 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] F.Okajima: "Intracellular cross-talk between thyrotropin receptor and A_1 adenosine receptor in regulation of phospholipase C and adenylate cyclase in COS-7 cells transfected with their receptor genes" Biochem.J.306. 709-715 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Shimegi: "Permissive stimulation of Ca^<2+> -induced phospholipase A_2 by an adenosine receptor agonist in a pertussis toxin-sensitive manner in FRTL-5 thyroid cells : a new 'cross-talk' mechanism in Ca^<2+> signalling" Biochem.J.299. 845-851 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Tomura: "Transfected human somatostain receptor type 2, SSTR2, not only inhibits adenylate cyclase but also stimulates phospholipase C and Ca^<2+> mobilization" Biochem.Biophys.Res.Commun. 200. 986-992 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Akbar: "A single of A_1 adenosine receptor expressed in chinese hamster ovary cells not only inhibits cAMP accumulation but also stimulates phospholipase C and arachidonate release" Mol.Phamacol. 45. 1036-1042 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Akbar: "Phospholipase C activation and Ca^<2+> mobilization by cloned human somatostain receptor subtypes 1-5, in transfectes COS-7 cells" FEBS Lett.348. 192-196 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Shimura: "Thyroid-specific expression and cyclic adenosine 3', 5'-monophosphate autoregulation of the thyrotropin receptor gene involves thyroid transcription factor-1" Mol.Endocrinology. 8. 1049-1069 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] F.Okajima: "Genistein, an inhibitor of protein tyrosine kinase, is also a competitive antagonist for P^1-purinergic (adenosine) receptor in FRTL-5 thyroid cells" Biochem.Biophys.Res.Commun.203. 1488-1495 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] F.Okajima: "Enkephalin activates phospholipase C-Ca^<2+> system through a cross-talk between opioid receptors and P_2-purinergic or bradykinin receptors in NG 108-15 cells : A permissive role for pertussis toxin-sensitive G-proteins" Biochem.J.290. 241-247 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.A.Majid: "UTP activates phospholipase C-Ca^<2+> system through a receptor different from the 53-kDa ATP receptor in PC12 cells" Biochem.Biophys.Res.Commun. 195. 415-421 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] A.Hidaka: "Receptor cross-talk can optimize assays for antoantibodies to the thyrotropin receptor : Effect of phenylisopropyladenosine on adenosine 3', 5'-monophosphate and inositol phosphate levels in rat FRTL-5 thyroid cells" J.Clin.Endocrinol.Metab.77. 1164-1169 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Shoda: "Graves'immunoglobulin G stimulates iodide efflux in FRTL-5 thyroid cells" J.Clin.Endocrinol.Metab.77. 94-97 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Kosugi: "Substitutons of different regions of the third cytoplasmic loop of the thyrotropin (TSH) receptor have selective effects on constitutive, TSH-, and TSH receptor autoantibody-stimulated phosphoinositide and 3', 5'-cyclic adenosine monophosphate signal generation" Mol.Endocrinol. 7. 1009-1020 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Sato: "Extracellular ATP stimulates three different receptor signal transduction systems in FRTL-5 thyroid cells" Biochem.J.283. 281-287 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] F.Okajima: "Synergism in cytosolic Ca^<2+> mobilization between bradykinin and agomists for pertussis toxin-sensitive G-protein-coupled receptors in NG 108-15 cells" FEBS Lett.301. 223-226 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Tomura: "Discrimination between two types of P^2 purinoceptors by suramin in rat hepatocytes" Eur.J.Pharmacol. 226. 363-365 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.A.Majid: "Characterization of ATP receptor which mediates norepinephrine release in PC12 cells" Biochimica et Biophysica Acta. 1136. 283-289 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Tomura: "Enkephalin induces Ca^<2+> mobilization in single cells of bradykinin-sensitized differentiated neuroblastoma hybridoma (NG108-15) cells" Neurosci.Lett.148. 93-96 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Tada: "P_2 purinoceptor-mediated cyclic AMP accumulation in bovine vascular smooth muscle cells" Eur.J.Pharmacol. 227. 25-31 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Kosugi: "Mutation of alanine 623 in the third cytoplasmic loop of the rat thyrotoropin (TSH) receptor results in a loss in the phosphoinositide but not cAMP signal induced by TSH and receptor autoantibodies" J.Biol.Chem.267. 24153-24156 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Sho: "Cooperation of TSH but not basic fibroblast growth factor with an adenosine receptor agonist in Ca^<2+> mobilization from thapsigargin sensitive pools in single FRTL-5 thyroid cells." Endocrinology. 136. 770-778 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Kimura: "TSH-induced hydrogen peroxide production in FRTL-5 thyroid cells is mediated not by cAMP but by Ca^<2+> signaling followed by phospholipase A_2 activation and potentiated by an adenosine derivative." Endocrinology. 136. 116-123 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Shimegi: "Permissive stimulation of Ca^<2+>-induced phospholipase A_2 by an adenosine receptor agonist in a pertussis toxin-sensitive manner in FRTL-5 thyroid cells: a new ‘cross-talk'mechanism in Ca^<2+> signalling." Biochem.J.299. 845-851 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Tomura: "Transfected human somatostatin receptor type 2, SSTR2, not only inhibits adenylate cyclase but also stimulates phospholipase C and Ca^<2+> mobilization." Biochem.Biophys.Res.Commun.200. 986-992 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Akbar: "A single species of A_1 adenosine receptor expressed in chinese hamster ovary cells not only inhibits cAMP accumulation but also stimulates phospholipase C and arachidonate release." Mol.Pharmacol.45. 1036-1042 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] F.Okajima: "Genistein, an inhibitor of protein tyrosine kinase, is also a competitive antagonist for P_1-purinergic(adenosine)receptor in FRTL-5 thyroid cells." Biochem. Biophys. Res. Commun.203. 1488-1495 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 岡島 史和: "甲状腺細胞におけるアデノシンとTSHの共同作用と生理・病理的意義" 永井書店, 4 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kosugi,S.: "Mutation of alanine 623 in the third cytoplasmic loop of the rat thyrotoropin(TSH)receptor results in a loss in the phosphoinositide but not cAMP signal induced by TSH and receptor autoantibodies." J.Biol.Chem.267. 24153-24156 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Okajima,F.: "Enkephalin activates phospholipase C-Ca^<2+> system through a cross talk between opioid receptors and P_2-purinergic or bradykinin receptors in NG 108-15 cells." Biochem.J.290. 241-247 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.A.Majid: "UTP activates phospholipase C-Ca^<2+> system through a receptor different from the 53-kDa ATP receptor in PC12 cells." Biochem.Biophys.Res.Commun.195. 415-421 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hidaka,A.: "Receptor cross-talk can optimize assays for autoantibodies to the thyrotropin receptor:effect of phenylisopropyladenosine on adenosine 3',5'-monophosphate and inositol phosphate levels in rat FRTL-5 thyroid cells." J.Clin.Endocrinol.Metab.77. 1164-1169 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kosugi,S.: "Substitutions of different regions of the third cytoplasmic loop of the thyrotropin(TSH)receptor have selective effects on constitutive,TSH-,and TSH receptor autoantibody-stimulated phosphoinositide and 3',5'-cyclic adenosine monophosphate signal generation." Mol.Endocrinol.7. 1009-1020 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shoda,Y.: "Graves' immunoglobulin G stimulates iodide efflux in FRTL-5 thyroid cells." J.Clin.Endocrinol.Metab.77. 94-97 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 近藤洋一: "甲状腺ホルモンの生合成と分泌「最新内科学大系13,甲状腺疾患」" 中山書店, 18 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 近藤洋一: "サイログロブリンと抗サイログロブリン抗体検査と技術" 2 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sato,K.: "Extracellular ATP stimulates three different receptor signal transduction systems in FRTL-5 thyroid cells." Biochem.J.283. 281-287 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Okajima,F.: "Synergism in cytosolic Ca^<2+> mobilization between bradykinin and agonists for pertussis toxin-sensitive G-protein-coupled receptors in NG 108-15 cells." FEBS Lett.301. 223-226 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tomura,H.: "Discrimination between two types of P_2 purinoceptors by suramin in rat hepatocytes." E.J.Pharmacol.226. 363-365 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.A.Majid: "Characterization of ATR receptor which mediates norepinephrine release in PC12 cells." Biochimica et Biophsica Acta.1136. 283-289 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tomura,H.: "Enkephalin induces Ca^<2+> mobilization in single cells of bradykinin-sensitized differentiated neuroblastoma hybridoma (NG108-15)Cells." Neurosci.Lett.148. 93-96 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tada,S.: "P_2 purinoceptor-mediated cyclic AMP accumulation in bovine vascular smooth muscle cells." E.J.Pharmacol.227. 25-31 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 近藤 洋一: "新生化学実験講座 9,ホルモンII非ペプチドホルモン" 東京化学同人, 6 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 近藤 洋一: "新生化学実験講座 9,ホルモンII非ペプチドホルモン" 東京化学同人, 8 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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