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The equilibrium and control of cell proliferation, Survival and death in chronic hematological neoplasms

Research Project

Project/Area Number 04454573
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionKumamoto University

Principal Investigator

TAKATSUKI Kiyoshi  Kumamoto University, School of Medicine Professor, 医学部, 教授 (80026830)

Co-Investigator(Kenkyū-buntansha) ASOU Norio  Kumamoto University, School of Medicine Lecturer, 医学部, 講師 (50175171)
MATSUZAKI Hiromitsu  Kumamoto University Hospital Lecturer, 医学部・付属病院, 講師 (30136725)
藤本 幸示  熊本大学, 医学部・附属病院, 講師 (20190069)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1993: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1992: ¥3,500,000 (Direct Cost: ¥3,500,000)
Keywordschronic hematological neoplasms / CLL / ATL / apoptosis / p53 / EBV / 細胞周期 / 細胞増殖 / 細胞死 / インターロイキン2 / T細胞受容体 / 顆粒リンパ球白血病 / EBV / Fas抗原 / p53遺伝子
Research Abstract

In chronic hematological neoplasmas such as chronic lymphocytic leukemia (CLL) and chronic type of adult T-cell leukemia (ATL) , it is likely that cell proliferation, survival and death keep good equilibrium in each. The aim of this study is analysis of the relationship between cell prolifeartion and death, particulaly programd cell death(apoptosis), in order to clarify the pathological features and to find new approach to therapy in chronic hematological neoplasms. Firstly, we established the serum free culture system in which we can examine several factors assoiated with the apoptosis. In CLL and ATL, we found that apoptosis is increased by corticosteroid and protein kinase A activator and decreased by interleukin 2. In ATL, we detected the mutation and deletion of the p53 gene, which regulate cell cycle at the Gl check point and control apoptosis. The alteration of p53 gene is frequently observed in acute and crisis type of ATL but not in chronic type, suggesting that the p53 gene alteration is assosiated with disease progression.In addition, we found EBV genome as a single episomal form is 4 out of 34 patients with T-cell lymphoma and leukemia, indicating that latent gene products of EBV or celluler oncogenes are involved in the development of Japanese T-cell neoplasm following EBV infection. These observations indicate that the several inner and outer factors regulate cell proliferation and death in chronic hematological neoplasms.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (40 results)

All Other

All Publications (40 results)

  • [Publications] Akiko Sakashita,et al.: "Mutations of the p53 gene in adult T-cell leukemia." Blood. 79. 477-480 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hitoshi Suzushima,et al.: "Detection of Epstein-Barr viralDNA in aggressive CD+ T cell leukemic cells." Brit.J.Haematol.82. 774-776 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hitoshi Suzushima,et al.: "Double-negative(CD4-,CD8-)T cells from adult T-cell leukemia patients also have poor expression of the T-cell receptor αβ/CD3 complex." Blood. 81. 1032-1039 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Norio Asou,et al.: "Inversion of chromosome 16 and bone marrow eosinophilia in a myelomonocytic tranaformation of chronic myeloid leukemia." Cancer Genet.Cytogenet.61. 197-200 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ren-Wei Huang,et al.: "Interleukin-2 prevents programmed cell death in chronic lymphocytic leukemia cells." Int.J.Hematol.58. 83-92 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiroyuki Tsuda,et al.: "Interleukin-2 prevents programmed cell death in adult T-cell leukemia cells." Jpn.J.Cancer Res.84. 431-437 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiroyuki Tsuda,et al.: "Programmed cell death and its control in the development of adult T-cell leukaemia:Hypothesis." 0ncol.Rep.1. 149-150 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ren-wei Huang,et al.: "A new derivative of camptothecin irinotecan hydrochloride (CPT-11)induces-programmed cell death in leukemia/lymphoma cell lines." Int.J.0ncol.3. 679-685 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hitoshi Suzushima,et al.: "CD4+ CD8+ granular lymphocytic leukemia arising in a patient with acute myeloblastic leukemia." Leukemia.8. 1884-1889 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hitoshi Suzushima,et al.: "Lack of the expression of EBNA-2 and LMP-1 in T-cell neoplasmsposessing Epstein-Barr virus." Blood. 85. 480-486 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shintaro Nishimura,et al.: "P53 gene mutation and loss of heterozygosity are associated with increased risk of disease progression in adult T-cell leukemia." Leukemia. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Norio Asou,et al.: ""The mechanism and new approachon drug resistance of cancer cells"" Elsevier Science Publisher B.V, 8 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Akiko Sakashita, Toshio Hattori, Carl W.Miller, Hitoshi Suzushima, Norio Asou, Kiyoshi Takatsuki and H.Phillip Koeffler: "Mutations of the p53 gene in a dult T-cell leukemia" Blood. 79. 477-480 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hitoshi Suzushima, Shuzo Matsuhsita, Ryotaro Nakamura, Shintaro Nishimura, Toshiya Okubo, Norio Asou, Keisei Kawa-Ha, Kanji Hirai, Kiyoshi Takatsuki: "Detection of Epstein-Barr viral DNA in aggressive CD8+T cell leukemic cells" Brit.J.Haematol. 82. 774-776 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hitoshi Suzushima, Norio Asou, Shintaro Nishimura, Kouji Nishikawa, Jian-xiang Wang, Toshiya Okubo, Makoto Naito, Toshio Hattori, and Kiyoshi Takatsuki: "Double-negative (CD4-, CD8-) T cells from adult T-cell leukemia patients also have poor expression of the T-cell receptor alphabeta/CD3 complex" Blood. 81. 1032-1039 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Norio Asou, Isao Sanada, Kenji Tanaka, Michihiro Hidaka, Hitoshi Suzushima, Hiromitsu Matsuzaki, Fumio Kawano, and Kiyoshi Takatsuki: "Inversion of chromosome 16 and bone marrow eosinophilia in a myelomonocytic transformation of chronic myeloid leukemia" Cancer Genet.Cytogenet. 61. 197-200 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ren-Wei Huang, Hiroyuki Tsuda, and Kiyoshi Takatsuki: "Interleukin-2 prevents programd cell death in chronic lymphocytic leukemia cells" Int.J.Hematol. 58. 83-92 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiroyuki Tsuda, Ren-Wei Huang, and Kiyoshi Takatsuki: "Interleukin-2 prevents programd cell death in adult T-cell leukemia cells" Jpn.J.Cancer Res. 84. 431-437 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiroyuki Tsuda, Ren-Wei Huang, and Kiyoshi Takatsuki: "Programd cell death and its control in the development of adult T-cell leukaemia : Hypothesis" Oncol.Rep. 1. 149-150 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ren-Wei Huang, Kiyoshi Takatsuki and Hiroyuki Tsuda: "A new derivative of camptothecin irinotecan hydrochloride (CPT-11) induces-programd cell death in leukemia/lymphoma cell lines" Int.J.Oncol. 3. 679-685 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hitoshi Suzushima, Norio Asou, Kenichiro Eto, Shintaro Nishimura, Toshiya Okubo, Tetsuhiro Fujimoto, Hiroshi Yamasaki, Motomi Osato, Motohiro Takeya, and Kiyoshi Takatsuki: "CD4+CD8+granular lymphocytic leukemia arising in a patient with acute myeloblastic leukemia" Leukemia. 8. 1884-1889 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hitoshi Suzushima, Norio Asou, Tetsuhiro Fujimoto, Shintaro Nishimura, Toshiya Okudo, Hiroshi Yamasaki Motomi Osato, Masao Matsuoka, Atsukamoto, Kazue Takai, Fumio Kawano, and Kiyoshi Takatsuki: "Lack of the expression of EBNA-2 and LMP-1 in T-cell neoplasms posessing Epstein-Barr virus." Blood. 85. 480-486 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shintaro Nishimura, Norio Asou, Hitoshi Suzuhima, Toshiya Okubo, Tetsuhiro Fujimoto, Motomi Osato, Hiroshi Yamasaki, Li Lisha, Kiyoshi Takatsuki: "P53 gene mutation and loss of heterozygosity are associated with increased risk of disease progression in adult T-cell leukemia" Leukemia. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Norio Asou, Koji Nishikawa, Hitoshi Suzushima, Jian-xiang Wang, Shintaro Nishimura, Toshiya Okubo, Fumio Kawano, Toshio Hattori, and Kiyoshi Takatsuki: Low level expression of multidrug resistance l mRNA detected by RT-PCR in acute leukemias. Elsevier Science Publisher B.V."The mechanism and new approach on drug resistance of cancer cells", 13-20 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Tsuda,et al.: "Programmed cell death and its control in the development of adult T-cell leukaemia:Hypothesis" Oncol.Rep.1. 149-150 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] R-W Huang,et al.: "A new derivative of camptothecin,irinotecan hydrochloride(CPT-11)induces-programmed cell death in leukemia/lymphoma cell lines" Int.J.Oncol.3. 679-685 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Suzushima,et al.: "CD4^+CD8^+ granular lymphocytic leukemia arising in a patient with acute myeloblastic leukemia" Leukemia. 8. 1884-1889 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Suzushima,et al.: "Lack of the expression of EBNA-2 and LMP-1 in T-cell neoplasms posessing Epstein-Barr virus." Blood. 85(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Nishimura,et al.: "p53 gene mutation and loss of heterozygosity are associated with increased risk of disease progression in adult T-cell leukemia." Leukemia. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Hata,et al.: "Establishment of a CD45-positive immature plasma cell line from an aggressive multiple myeloma with high serum lactate dehydrogenase." Leukemia. 8. 1768-1773 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] R-W Huang,K Takatsuki: "Interleukin-2 prevents programmed cell death in chronic lymphocytic leukemia cells" Int.J.Hematol.58. 83-92 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Tsuda,K.Takatsuki: "Interkeukin-2 prevents programmed cell death in adult T-cell leukemia cells" Jpn.J.Cancer.Res.84. 431-437 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] N Asou,K.Takatsuki: "Low evel expression of multidrug resistance 1 mRNA detected by RT-PCR" Elsevier Science Publishcr B.V.“The mechanism and new approach on drung resistance of cancer cells". 13-20 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M Yoshida,K Takatsuki: "Neutrophil chemotactic factors produced by malignant fiorous hitsiocytoma cell lines" Br.J.Cancer.67. 508-513 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Hata,K Takatsuki: "Expression of fibronectin and adnesion to fibronectiin in myeloma cell lines" Acta Haematol. 89. 26-31 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] F.Matsuno,K Takatsuki: "A novel omithine transcarbamylase prsesnt in mycoolasma-infected myeloma cells" Enzyme & Protein. 47. 57-64 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Akiko Sakashita,Kiyoshi Takatsuki,: "Mutations of the p53 qene in adult T-cell leukemia" Blood. 79. 477-480 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Norio Asou,: "Inversion of chromosome 16 and marrow eosinophilia in a myelomonocytic tranformation of chronic myeloid leukemia" Cancer Genet. Cytogenet. 61. 197-200 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hitoshi Suzushima,Kiyoshi Takatsuki,: "Detection of Epstein-Barr viral DNA in aggressive CD8+ T cell leukemic cells" Brit.J.Haematol.82. 774-776 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hitoshi Suzushima,Kiyoshi Takatsuki,: "Double-Negative(CD4-,CD8-)T cells from Adult T-cell leukemia patients also have poor expressioj of the T-cell receptor αβ/CD3 complex" Blood. 81. 1032-1039 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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