| Project/Area Number |
04454575
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Jichi Medical School |
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Principal Investigator |
SAITO Masaki Jichi Medical School, Professoor, 医学部, 教授 (60012762)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAI Takao Jichi Medical School, Associate, 医学部, 助手 (10235111)
FURUKAWA Yusuke Jichi Medical School, Lecturer, 医学部, 講師 (00199431)
NAKAMURA Mitsuru JIchi Medical School, Lecturer, 医学部, 講師 (20198237)
OHTA Masatsugu jichi Medical School, Lecturer, 医学部, 講師 (90160514)
KITAGAWA Sei-ichi Jichi Medical School, Assoc. Professoor, 医学部, 助教授 (50133278)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1993: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1992: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | Cell Cycle / cdc2 Gene Product / E2F Binding Site / Glycoprotein Tenascin / Extracellular Matrix / Nonepithelial Stroma Cells / Cell Growth Factors / Change Chromatin Structure / クロマチン構造変化 / 細胞外マトリックス / 非上皮系間質細胞 / 増殖因子 / (非)上皮系細胞 |
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| Research Abstract |
Our recent research works brought about the following results : (1) Cdc2 mRNA transcript wasreadily detected in immature bone marrow cells and became undetectable accompanying with the cell cycle arrest which ocurred along wich differentiation : it was undetectable after the myelocyte/metamyelocyte stages in granulocytic differentiation. Peripheral blood resting cells including granulocytes, monocytes and T-lymphocytes did not express cdc2 mRNA transcripts. Cdc2 mRNA could be induced in T-lymphocytes when the cells re-entered the cell cycle in response to specific mitogens such as PHA.In contrast, cdc2 mRNA could not be induced in granulocytes and monocytes even after the culture with the appropriate stimulants such as LPS or CSFs. The changes in the chromatin structure such as the appearance of DNase I hypersensitivity sites or the slteration of DNA methylation status were not observed in T-lymphocytes even after the induction of cdc2 mRNA expression by mitogenic stimuli. Then, we isolated the regulatory sequence of cdc2 gene by a ligation-mediated PCR and found E2F binding site at the position of nt -117 to -124 and the RB control elements at the positions of nt -106 to -112 and -156 to -165. (2) Tenascin is a novel six-armed extracellular-matrix glycoprotein expressed in association with mesenchymal-epithelial interactions, and its expression is temporally restricted during oganogenesis and carcinogenesis. Immuoprecipitation studies revealed two ascites-hepatoma-derived cell lines and one sarcoma-derived line synthesized tenascin in vitro. The other cell lines examined, including all of those derived from normal hepatocytes, were negative for the expression of tenascin. Coculture studies were performed between epithelial and nonepithelial cell lines. No drastic change in tenascin expression was found after cocultuing the cells. As an in vivo study, cell lines were transplanted into nude mice. All xenografts of the epithelial lines were associated with a storong
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