| Project/Area Number |
04454591
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
IRIMURA Tatsuro The University of Tokyo, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (80092146)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Kazuo Faculty of Pharmaceutical Sciences, Assistant Professor, 薬学部, 助手 (20174782)
IMAI Yasuyuki Faculty of Pharmaceutical Sciences, Assistant Professor, 薬学部, 助手 (80160034)
TSUJI Tsutomu Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (00143503)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1993: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1992: ¥3,400,000 (Direct Cost: ¥3,400,000)
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| Keywords | Sialyl-Le^x / Macrophage lectin / Poly-N-acetyllactosamine / Tumor antigen / Metastasis / Selectin / レクチン / 細胞認識 / マクロファージ / 白血球 / セリン / スレオニン結合型糖鎖 / 細胞交通 / lectin / selectin / carbohydrate / recognition / macrophoge / platelet |
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| Research Abstract |
The cellular recognition involved in a variety of pathological processes such as, inflammation, tissue reorganization, vascular injury, angiogenesis, and metastasis of tumor cells is mediated by carbohydrate-protein interactions. To understand these processes, the structure and the function of cell surface glycoconjugates and lectins should be elucidated. In the present project, we focused on the following specific aims and made interesting observasions : (1)The structure and the function of macrophage galactose/N-acetylgalactosamine-specific lectins. We concluded that the carbohydrate specificity of this lectin towards Tn-antigen and highly branched N-linked carbohydrate chains were suitable to distinguish tumor cell surfaces from non-malignant cell surfaces. (2)We found that sialyl-Le^x carbohydrate chains linked to serine and/or threonine were responsible for the platelet-induced superoxide anion production. (3) We purified an N-acetylglucosaminyltransferase involved in the formation of poly-N-acetyllactosamine-type carbohydrate chains.
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