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Dynein arm-function studied by mutants and in vitro motility

Research Project

Project/Area Number 04454608
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 分子遺伝学・分子生理学
Research InstitutionThe University of Tokyo

Principal Investigator

KAMIYA Ritsu  The University of Tokyo Graduate School of Science Professor, 大学院・理学系研究科, 教授 (10124314)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1992: ¥4,900,000 (Direct Cost: ¥4,900,000)
KeywordsChlamydomonas / dynein / flagella / mutant / motility / 鞭毛運動 / 細胞運動
Research Abstract

Dynein plays a most important function in the flagellar beating mechanism. Flagellar dynein comprises as many as ten different heavy chains in the inner and outer arms, but the specific function of each heavy chain is unknown. To approach the function of various heavy chains, we carried out two lines of experiments : isolation Chlamydomonas mutants that lack particular heavy chains, and assay of the motile activity of isolated heavy chains.
In the first approach, we isolated a mutant that lacks part of the beta heavy chain of the outer arm and two kinds of mutants that lack different sets of inner-arm heavy chains. Studies on these mutants have indicated that (1) the beta heavy chain is more important than the alpha heavy chain in the function of outer arm dynein, and (2) five out of the eight known inner-arm heavy chains are unnecessary for the generation of flagellar beating.
In the second line of study, we first developed a system in which dissociated outer-arm dynein, an alpha/beta subparticle and a gamma subparticle, can be recombined and attached to an outer arm-less mutant, oda1. This study again demonstrated that the beta heavy chain is important in the outer arm function. Finally, we developed an in vitro motility assay system using isolated inner arm dynein subspecies. Six out of the seven subspecies (containing eight heavy chains) were found to have activities to translocate microtubules in vitro. Different subspecies differ in maximal velocity and in the ability to cause microtubule rotation. These studies indicate that the flagella contain various dyneins that differ functionally. How these different dyneins cooperate in producing regular flagellar beating is a fascinating future problem.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Kamimura,S.: "High-frequency vibration in flagellar axonemes with amplitudes reflecting the size of tubulin." Journal of Cell Biology. 116. 1443-1454 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kamiya,R.: "Molecular mechnism of ciliary and flagellar movement." Advances in Comparative and Environmental Physiology. 12. 206-226 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takada,S.: "Three-headed outer arm dynein from Chlamydomonas that can functionally combine with outer arm-missing axonemes." Journal of Biochemistry. 111. 758-762 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kagami,O.: "Translocation and rotation of microtubules caused by multiple species of Chlamydomonas inner-arm dynein." Journal of Cell Science. 103. 653-664 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sakakibara,H.: "A Chlamydomonas outer arm dynein mutant with a truncated beta heavy chain." Journal of Cell Biology. 122. 653-662 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kato,T.: "Isolation of two species of Chlamydomonas reinhardtii flagellar mutants,ida5 and ida6,that lack a newly identified heavy chain of the inner dynein arm." Cell Structure and Function. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kamimura, S.and Kamiya, R.: "High-frequency vibration in flagellar axonemes with amplitudes reflecting the size of tubulin." J.Cell Biol.116. 1443-1454 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kamiya, R.: "Molecular mechnism of ciliary and flagellar movement." Advances in Comparative and Environmental Physiology. 12. 206-226 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takada, S., Sakakibara, H.and Kamiya, R.: "Three-headed outer arm dynein from Chlamydomonas that can functionally combine with outer arm-missing axonemes." J.Biochem.111. 758-762 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kagami, O.and Kamiya, R.: "Translocation and rotation of microtubules caused by multiple species of Chlamydomonas inner-arm dynein." J.Cell Sci.103. 653-664 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sakakibara, H., Takada, S., King, S.M., Witman, G.B.and Kamiya, R.: "A Chlamydomonas outer arm dynein mutant with a truncated beta heavy chain." J.Cell Biol.122. 653-662 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kato, T., Kagami, O., Yagi, T.and Kamiya, R.: "Isolation of two species of Chlamydomonas reinhardtii flagellar mutants, ida5 and ida6, that lack a newly identified heavy chain of the inner dynein arm." Cell Struct.and Funct.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sakakibara,H.: "A Chlamydomonas outer arm dynein mutant with a truncated beta heavy chain." Journal of Cell Biology. 122. 653-662 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kato,T.: "Isolation of two species of Chlamydomonas reinhardtii flagellar mutants,ida5 and ida6,that lack a newly identified heavy chain of the inner dynein arm" Cell Structure and Function. (印刷中).

    • Related Report
      1993 Annual Research Report
  • [Publications] Kamimura,S.: "High-frequency vibration in flagellar axonemes with amplitudes reflecting the size of tubulin" Journal of Cell Biology. 116. 1443-1454 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takada,S.: "Three-headed outer arm dynein from Chlamydomonas that can functionally combine with outer armmissing axonemes" Journal of Biochemistry. 111. 758-762 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kagami,O.: "Translocation and rotation of microtubules caused by multiple species of Chlamydomonas inner-arm dynein" Journal of Cell Science. 103. 653-664 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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