| Project/Area Number |
04454614
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | NATIONAL INSTITUTE OF GENETICS |
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Principal Investigator |
ISHIHAMA Akira National Institute of Genetics, Department of Molecular Genetics, Professor and Head, 分子遺伝研究系, 教授 (80019869)
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| Co-Investigator(Kenkyū-buntansha) |
TOYODA Tetsuya National Institute of Genetics, Department of Molecular Genetics, Assistant Prof, 分子遺伝研究系, 助手 (00197972)
YAMAGISHI Masahiro National Institute of Genetics, Department of Molecular Genetics, Assistant Prof, 分子遺伝研究系, 助手 (00220252)
FUJITA Nobuyuki National Institute of Genetics, Department of Molecular Genetics, Assistant Prof, 分子遺伝研究系, 助手 (90173434)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1993: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1992: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | RNA polymerase / Transcription regulation / Transcription factor / Protein-protein contact / Structure-function relationship / Escherichia coli / Molecular anatomy / 酵母 / 転写調節 / 構造-機能相関 / 蛋白-蛋白相互作用 / 分子集合 / 蛋白-DNA相互作用 |
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| Research Abstract |
Transcription is regulated by controlling the promoter selectivity of RNA polymerase. Bacterial RNA polymerase is composed of four major subunits, 2alpha, 1beta, 1beta' and 1sigma subunit. In order to understand how the promoter recognition properties of RNA polymerase is controlled by interaction with transcription factors, we carried out in this research the molecular anatomy of Escherichi coli RNA polymerase, in particular focussing on the contact site mapping on RNA polymerase subunits with various transcription factors. Results indicate that transcription factors can be classified based on the contact site on RNA polymerase. Class-I transcription factors were found to contact with the contact site I located at the C-terminal region of alpha subunit, while class-II factors make contact with the contact site II clustered in the C-terminal region of sigma subunit. Fine mapping revealed that the protein-protein communications between RNA polymerase and transcription factors involve narrow regions on both sides, each being composed of epitope size consisting of about 10 amino acid residues. In parallel, contact site mapping between RNA polymerase subunits has also been carried out.
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