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Analysis of Intercellular Interaction in a Process of Neurite Growth

Research Project

Project/Area Number 04454617
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 生物物性学
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

MATSUOKA Hideaki  Tokyo Univ.Agric.Technol., Faculty of Technology, Prof., 工学部, 教授 (10143653)

Co-Investigator(Kenkyū-buntansha) 根本 泰行  東京農工大学, 工学部, 講師 (70202249)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1993: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsPC12h-R / Lone cell culture / Flow culture system / Conditioned medium / Electrical effect / Nerve growth factor / db-cAMP / Neurite growth / 神経成長因子 / 共存細胞効果 / 細胞間相互作用 / 面像処理 / 定量的評価
Research Abstract

It is known that a neural cell elongates neurites and make a synaptic combination with a target cell. In a process of this neurite growth, some intercellular interaction might be worked between neurons. In order to analyze this intercellular interaction, the present research project was done from 1992 to 1994.
In 1992, using the cultured nerve cells PC12h-R,we developed lone cell culture system which excluded the intercellular interaction absolutely because only one cell was cultivated in a culture well. The usefulness of this system for further research work was shown.
In 1993, a neurite growth initiating activity of various factors (neurotrophic factors, neurotransmitter, hormone, second messenger) was evaluated by using lone cell system. Moreover, a flow culture system which could change the kind and concentration of working factors continuously and satisfied the lone cell culture condition was developed.
In 1994, we analyzed the chemical and physical factors of intercellular interaction by using the developed culture systems. As a chemical factor, some protein, whose molecular weight was 130-170kDa and which induced the neurite growth, was found in the supernatant of the conditioned medium of PC12h-R.It was shown that the stimulation by an alternating current (intensity : >2 muA) also induced the neurite growth. Moreover, in PC12h-R cells, the response of neurites to the repeated actions of NGF or db-cAMP whose action mechanisms were different to neural cells was analyzed.
These results were new knowledge and could be obtained by using our developed culture system. It was concluded that both chemical and physical factors were complicatedly related to the intercellular interaction in the process of the neurite growth.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Yasushi Kazuno: "Use of Lone Cell of PC12h-R as a Sensing Material for Neurite Growth Initiating Activity" J.Intel.Master.Syst.Struct.5. 117-121 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Jong-Chul Park: "Adaptation of Aspergillus niger to Several Antifungal Agents." Microbiology. 140. 2409-2414 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hideaki Matsuoka: "Adaptation of Aspergillus niger to Multiple Agents Whose Action Mechanisms are Different." Jpn.J.Med.Mycol. 36. 19 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hyeong-Cheol Yang: "Application of a Microscopic Fluorescence Indicator to the Determination of Antimycotic Activity." J.Antiboct.Antifung.Agents. 23. 3-7 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hideaki Matsuoka: "Use of Congo Red as a Microscopic Fluorescence Indicator of Hypha Growth." Appl.Microbiol.Biotechnol.41(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hyeong-Cheol Yang: "Rapid Viability Assessment of Spores of Several Fungi by Ionic Intensified FDA Method." Current Microbiol. 30(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasushi Kazuno: "Use of Lone Cell of PC12h-R as a Sensing Material for Neurite Growth Activity." J.Intel.Mater.Syst.Struct.5. 117-121 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Jong-Chul Park: "Adaptation of Aspergillus niger to Several Antifungal Agents" Microbiology. 140. 2409-2414 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hideaki Matsuoka: "Adaptation of Aspergillus niger to Multiple Agents Whose Action Mechanisms are Different." Jpn.J.Med.Mycol.36. 19-24 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hyeong-Cheol Yang: "Application of a Microscopic Fluorescence Indicator to the Determination of Antimycotic Activity." J.Antibact.Antifung.Agents. 23. 3-7 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hideaki Matsuoka: "Use of Congo Red as a Microscopic Fluorescence Indicator of Hypha Growth" Appl.Microbiol.Biotechnol.41 (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hyeong-Cheol Yang: "Rapid Viability Assessment of Spores of Several Fungi by Ionic Intensified FDA Method." Current Microbiol.30 (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Jong-Chul Park: "Adaptation of Aspergillusniger to Several Antifungal Agents." Microbiology. 140. 2409-2414 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yasushi Kazuno: "Use of Lone Cell of PC12h-R as a Sensing Material for Neurite Growth Initiating Activity." J.Intel.Mater.Syst.Struct.5. 117-121 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hideaki Matsuoka: "Adaptation of Aspergillusniger to Multiple Agents Whose Action Mechanisms are Different." Jpn.J.Med.Mycol.36. 19-24 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hyeong-Cheol Yang: "Application of a Microscopic Fluorescence Indicator to the Determination of Antimycotic Activity." J.Antibact.Antifung.Agents. 23. 3-7 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hideaki Matsuoka: "Use of Congo Red as a Microscopic Fluorescence Indicator of Hypha Growth." Appl.Microbiol.Biotechnol.41(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Hyeong-Cheol Yang: "Rapid Viability Assessment of Spores of Several Fungi by Ionic Intensified FDA Method." Current Microbiol.30 (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Oh,H.Matsuoka,T.Teraoka,O.Sumita,K.Takatori,H.Kurata: "Effect of Antimycotics on the Biosynthesis of Cellular Macromolecules in Aspergillus niger Protoplasts." Mycopathologia. 122. 135-141 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Matsuoka,Y.Kazuno,T.Horie,T.Homma,Y.Nemoto: "Effect of Coexisting cells on the NGF-Inducing Neurite Growth from PC12h-R." Cytotechnology. 11. 59-65 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Oh,H.Matsuoka,Y.Nemoto,O.Sumita,K.Takatori,H.Kurata: "Determination of Anti-Aspergillus Activity of Antifungal Agents Based on the Dynamic Growth Rate of a Single Hypha." Appl.Microbiol.Biotechnol.39. 363-367 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Kazuno,T.Homma,Y.Nemoto,H.Matsuoka: "Development of a Flow System for the Microscopic Observation of Cultured Nerve Cells and Its Application to the Neurite Growth Control of PC12h-R." Biochim.Biophys.Acta.1178. 299-301 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] J,C.Park,Y.Nemoto,T.Homma,W.Jing,Y.Chen,H.Matsuoka,H.Ohno,K.Takatori,H.Kurata: "Adaptation of Aspergillus niger to Short-term Salt Stress." Appl.Microbiol.Biotechnol.40. 394-398 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Yamada,J.Cao,O.Sumita,K.Kurosawa,H.Kurata,K.Oh,H.Matsuoka: "Automatic Antifungal Analyzing System on the Basis of Dynamic Growth Process of a Single Hypha" Mycopathologia. 118. 65-69 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Oh,H.Matsuoka,O.Sumita,K.Takatori,H.Kurata: "Evaluation of Antifungal Activity of Antimycotics by Automatic Analyzing System" Mycopathologia. 118. 71-81 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Oh,H.Matsuoka,O.Sumita,K.Takatori,H.Kurata: "Automatic Evaluation of Antifungal Volatile Compounds on the Basis of Dynamic Growth of a Single Hypha" Appl.Microbiol.Biotechnol.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Oh,H.Matsuoka,Y.Nemoto,O.Simuta,K.Takatori,H.Kurata: "Determination of Anti-Aspergillus Activity of Antifungal Agents Based on the Dynamic Growth Rate of a Single Hypha" Appl.Microbiol.Biotechnol.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Matsuoka,Y.Kazuno,T.Hoirie,T.Homma,Y.Nemoto: "Effect of Coexisting Cells on the NGF-inducing Neurite Growth from PC12h-R" Cytotechnology. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 松岡 英明,根本 泰行,本間 知夫,呉 基鳳,澄田 修生,山田 暁,大野 浩和,井川 勇,松沼 英雄,高鳥 浩介,倉田 浩: "抗真菌活性評価システムの開発" 防菌防黴. 21. 99-105 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 松岡 英明: "細胞成長の動的計測" 日本農薬学会誌. 18. S1-S5 (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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