| Project/Area Number |
04454620
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
生物物性学
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| Research Institution | Osaka University |
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Principal Investigator |
KASAI Michiki OSAKA Univ., FAC.ENGINEER.SCI., PROFESSOR, 基礎工学部, 教授 (40022595)
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| Co-Investigator(Kenkyū-buntansha) |
IDE Toru OSAKA Univ., FAC.ENGINEER.SCI., ASSISTANT, 基礎工学部, 助手 (60231148)
TAGUCHI Takahisa OSAKA Univ., FAC.ENGINEER.SCI., ASSISTANT, 基礎工学部, 助手 (10197246)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1992: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | Ion channel / Lipid bilayr / K channel / CI channel / Sarcoplasmic reticulum / Ca channel |
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| Research Abstract |
Purification of ion channels in sacrcoplasmic (SR) of rabbit skeletal muscle has been carried out by assaying the channel activity in the lipid bilayr system. As a result, the fraction which contains 138 and 100 kDa protein showed CI^- channel activity. After purification of 138 kDa protein, partial amino acid sequences were determined and their homegogy was referred. The 138 kDa protein was found to be the same one as that reported as 160 kDa protein which has Ca^<2+>-binding activity. The difference was due to the nuderestimation of the previous workers. The 138 kDa protein interacts with 100 kDa protein in SR.When 100 kDa protein was incorporated into lipid bilayr, it showed channel activity. It was suggested that 138 and 100 kDa proteins worked as ion channle by making complex.Antibody against 138 kDa protein inhibited slow KCI permaetion through SR vesicles.
CI^- channel activity in transverse tubule (T-tubule) in rabbit skeletal muscle was investigated by lipid bilayr system. A back-ground type CI^- channel (open at resting potential) having 40 pS was found. This channel was blocked by 9-AC,DIDS,SITS,and EA which were known as blockers againg voltage dependent anion channels. Further, the channel was blocked by IAA-94 which was developed as a blocker for CI^- channel in apical membrane from trachea. Similarity between these channels was suggested. Antibody which blocked the channel activity recognized 90 kDa protein in muscle.
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