| Project/Area Number |
04455020
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
広領域
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
KUNITAKE Toyoki Kyusyu Univ. Dept. Chemical Science and Technology, Professor, 工学部, 教授 (40037734)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Akio Kyushu Univ. Facult. Science Dept. Chemistry, Pofessor, 理学部, 教授 (30037379)
KIMIZUKA Nobuo Kyushu Univ. Dept. Chemical Science and Technology, Associate Professor, 工学部, 助教授 (90186304)
HAMACHI Itaru Kyushu Univ. Dept. Chemical Science and Technology, Associate Professor, 工学部, 助教授 (90202259)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Lipid Bilayr Membrane / Myoglobin / Lipid-Anchoring / Signal Sequence / Membrane-Bound Protein / Protein Assembly / 膜結合性蛋白質 / タンパク集積体 / 二分子膜 / 配向固定化 |
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| Research Abstract |
Directionally controlled anchoring of myoglobin, a water-soluble globular protein, on a dipalmitoylphosphatidylcholine(DPPC) bilayr has been successfully conducted. Lipid-cnchored myoglobin was obtained from a monoalkylated heme derivative and apomyoglobin by cofactor reconstituted method. It was indicated by gel filtration and ultrafiltration studies that the lipid-anchored myoglobin with a hydrophobic alkyl chain, but not native myoglobin and the hydrophilic-anchored myoglobin, is bound to the DPPC bilayr membrane in aqueous dispersion. A cast film of the phospholipid bilayr and the anchored myoglobin displayd anisotropic ESR signals, which depend on the disposition of the cast film in the magnetic field. These results suggest that myoglobin molecules are placed on the lipid bilayr in a fixed orientation by inserting the snchor alkyl chain into the bilayr. The ESR anisotropy was not observed without the anchor.
The present research clearly showed that lipid-anchor method is useful for oriented assembly of protein molecules in lipid bilayr membranes.
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