| Project/Area Number |
04455029
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
広領域
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| Research Institution | National Institute of Neuroscience, National Center of Neurology and Psychiatry |
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Principal Investigator |
OGINO Takashi National Institute of Neuroscience, NCNP,Head, 神経研究所, 室長 (50185526)
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| Co-Investigator(Kenkyū-buntansha) |
YANO Toshio National Institute of Neuroscience, NCNP,Research Fellow, 神経センター神経研究所, 研究員
SUGITA Hideo National Institute of Neuroscience, NCNP,President, 神経センター, 総長 (80009951)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1992: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | phosphorus / NMR / 2 Tesla / human / muscle / exercise / fiber-type / metabolism |
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| Research Abstract |
In this study, by the use of multinuclear NMR spectroscopy, we demonstrated that the energy metabolism of the specific human skeletal muscular fiber types(i. e., a single metabolic compartment within the muscle) could be non-invasively measured and that the detection of the Hb/Mb redox states in the human skeletal muscle could be possible. The characteristics of the present methods are as follows : (A) Construction of a non-magnetic ergometer for the human lower leg muscles used in a 2 Tesla wholebody super conducting magnet. (B) Non-invasive 1H gradient echo MRI detection of the muscular response to exercises in the human lower leg with the ergometer. (C) Construction of 2 Tesla 31P NMR surface coil probe for measurements of the triceps surae muscles in the ergometer. With aid of these devices, the following results were obtained : (1) With the intense loaded exercise protocol (2 Hz plantar flextions for 2 min against 6 kg weight loads), the changes of signal intensities of the gradient echo MRI were observed in the gastrocnemius muscles and the peroneus muscles, but no change was observed in the soleus muscle which is also part of the triceps surae muscles. (2) With the same loaded exercise protocol, the Pi (inorganic phosphate) resonance in the 31P NMR spectrum of the muscle split into three peaks with corresponding to three different pH values. The relative ratios of each peak area were changed by three months of training and returned to the original ones after detraining. (3) The onset of the exercise loading was followed by the low field shifts of all metabolite resonances both in the 1H and the 31P NMR spectra. The same shifts were observed in the experimentally induced ischemia of the muscle. The degree of the observed shifts was markedly large in the subjects with some practices of sports. This value was also changed by the three months of training. (4) The area of the triceps surae muscles measured by MRI increased after the execution of three months of t
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