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Rapid and Sensitive Microanalyzes of ^<13>C and ^<15>N tracers.

Research Project

Project/Area Number 04554027
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 生態学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

WADA Eitaro  Center for Ecological Research, Kyoto University, Professor, 生態学研究センター, 教授 (40013578)

Co-Investigator(Kenkyū-buntansha) YOSHIOKA Takahito  Institute of Hydrosphere and Atmosphere Sciences, Nagoya University, Instructor, 大気水圏科学研究所, 助手 (50202396)
SUGIMOTO Atsuko  Center for Ecological Research, Kyoto University, Instructor, 生態学研究センター, 助手 (50235892)
NAKANISHI Masami  Center for Ecological Research, Kyoto University, Associate Professor, 生態学研究センター, 助教授 (60025434)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥21,000,000 (Direct Cost: ¥21,000,000)
Fiscal Year 1993: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1992: ¥18,500,000 (Direct Cost: ¥18,500,000)
KeywordsGaschromatograph-Isotope Ratio Mass Spectrometer / Carbon isotope ratio / Nitrogen isotope ratio / Bubble methane / Total inorganic carbon / ^<13>C・^<15>N tracer / Fractionation of microorganisms / Natural abundances carbon and nitrogen isotopes / 炭素・窒素安定同位体のトレーサー / 質量分析法 / GC / C / IRMS / 気泡メタンの同位体比 / 全炭酸の同位体比 / ^<13>C, ^<15>Nトレーサー / 高精度質量分析 / 迅速分析 / ヘリウムフロースルー法 / 微小水界生物 / 琵琶湖 / 木崎湖 / 諏訪湖
Research Abstract

1. Gas chromatography/Combusion/Isotope Ratio Mass Spectrometer (GC/C/IRMS) was successfully established. The system was employed to develop a rapid and simple analytical methods for measuring carbon isotope ratio of bubble methane collected from wetlands, dissolved inorganic carbon (SIGMA CO_2) in lake water samples, and ^<13>C tracer studies of ^<13>C-CH_4 production from a rhizosphere of rice plants.
2. ^<15>N_2 and ^<13>C tracer studies were also developed for assessing the molecular dinitrogen fixation by the dry-wod termite, Neotermes koshuenies Shiraki, and the primary production by phytoplankton in Lake Biwa.
3. High vacuum line for the prepation of biological meterials were built and carbon and nitrogen isotope ratios were measured for various kinds of biogenic samples collected from L.Biwa, L.Suwa, L.Teganuma and a termite ecosystem.
4. Size fractionation of aquatic microorganisms was successfully performed by using a various kind of plankton net and soating by hand.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Wada,E.,Y.Kabaya,Y.Kurihara: "Stable isotopic structure of aquatic ecosystems." J.Biosci.18. 483-499 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sugimoto A.,E.Wada: "Carbon isotopic composition of bacterial methane in a soil incubation experiment:Contribution of acetate and CO_2/H_2." Geochim.Cosmochim.Acta. 57. 4015-4027 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 和田英太郎: "安定同位体は何を語るか" 遺伝. 47. 10-14 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 藤井・和田 他: "広がる安定同位体の利用" 日本原子力学会誌. 35. 952-977 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] E.Wada: "Possible impacts of acid rain on aquatic ecosystems from stable isotopic view points." Proceedings of the Int'l Workshop,国立環境研. 117-126 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] I.Tayasu,A.Sugimoto,E.Wada,T.Abe: "Xylophagons termites depending on atmospheric nitrogen" Natur wissen schaften. 80(in press). (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Aoki,T.,M.Kumagai,M.Nakanishi: "Development of DOC probe and application to water in L.Biwa" Verh.Internal.Verein.Limnol.25. 200-202 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nakano,S.,T.Miyajima,Y.Tezuka: "Abundance of algal picoplankton and hetero trophic flagellates in the south basin of L.Biwa,with special attention to nutrient level." Ann.Rept.Interdicipl.Res.Inst.Environ.Sci.12. 55-63 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nakanishi,M: "Influence of ezploitation of littoral zone on bio-communities in L.Biwa." Proceedings on “The Recent Advance in Ecosystem and Environmental Problem".(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 吉岡,和田,林,竹内,高橋: "メソコスム湖沼生態系の解析(安定同位体比から見た物質循環と食物網)" 西條八束,坂本充 編、名大出版会, 346 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 和田,杉本,吉岡: "水の惑星(水と生物)" 寺本俊彦 編著(オーム社), 134 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 中西正己: "人間活動と琵琶湖" 高橋正立,石田紀郎 編,世界思想社, 10 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 和田英太郎: "週刊朝日百科 動物たちの地球 142 地球共生系" 朝日新聞社, 4 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Wada, E., Y.Kabaya and Y.Kurihara: "Stable isotopic structure of aquatic ecosystems." J.Biosci.18. 483-499 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sugimoto A.and E.Wada: "Carbon isotopic composition of bacterial methane in a soil incubation experiment : Contribution of acetate and CO_2/H_2." Geochim.Cosmochim.Acta. 57. 4015-4027 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] E.Wada: "Possible impacts of acid rain on aquatic ecosystems from stable isotopic view points." Proceedings of the Int'l Workshop, National Institute for Environmental Studies. 117-126 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] I.Tayasu, A.Sugimoto, E.Wada, T.Abe: "Xylophagons termites depending on atmospheric nitrogen" Natur wissen schaften. 80(in press). (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Aoki, T., M.Kumagai, M.Nakanishi: "Development of DOC probe and application to water in L.Biwa" Verh.Internal.Verein.Limnol.25. 200-202 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nakano, S., T.Miyajima, Y.Tezuka: "Abundance of algal picoplankton and hetero trophic flagellates in the south basin of L.Biwa, with special attention to nutrient level." Ann.Rept.Interdicipl.Res.Inst.Environ.Sci.12. 55-63 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nakanishi, M.: "Influence of ezploitation of littoral zone on bio-communities in L.Biwa." Proceedings on "The Recent Advance in Ecosystem and Environmental Problem". (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Aoki,T.,M.Kumagai,M.Nakanishi: "Development of DOC probe and application to water in L.Biwa" Verh.Internal.Verein.Limnol.25. 200-202 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nakano,S.,T.Miyajima,Y.Tezuka: "Abundance of algal picoplankton and hetero trophic flagellates in the south basin of L.Biwa,with special attention to nutrient level." Ann.Rept.Interdicipl.Res.Inst.Environ.Sci.12. 55-63 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nakanishi,M.: "Influence of ezploitation of littoral zone on bio-communities in L.Biwa." Proceedings on “The Recent Advance in Ecosystem and Environmental Problem".(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 中西正己: "人間活動と琵琶湖" 高橋正立,石田紀郎編,世界思想社, 10 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 和田英太郎: "週刊朝日百科 動物たちの地球 142 地球共生系" 朝日新聞社, 4 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ichikawa,T.: "Nitrogen uptake rate of small zooplankton using ^<15>N tracer." Jpn.J.Limnal.53. 273-280 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 杉本 敦子: "生物圏における安定同位体の分布と地球環境" Radio isotopes. 41. 366-376 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takahashi,K.: "Carbon isotope ratio and photosyuthetic activity of phytoplankton in the eutrophic Mikawa Bay." Japan.Ecological Res.7. 355-361 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nakatsuka,T.: "The dynamic chauges of stable isotopic ratios of carbon and uitrogen in suspended and sedimented particulate organic matter during phytoplankton bloom." J.Mar.Res.50. 267-296 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Watanabe,I.& Wada,E.: "Nitrogen fixation in flooded rice soils and aquatic,and sedimentsystems.In‘Nitrogen Isotope Techniques.'R.Knoules and T.H.Blackburn Eds.157-178" Academic Press,Inc., 311 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 吉岡 崇仁: "「川と湖の生物-多様性と相互使用」林秀剛他2名編著、第11章物静かな湖の語り部P.199-215" 信濃毎日新聞社, 270 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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