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PRECISE QUANTIZATION OF PHASE CONTRAST MICROSCOPE FOR OBSERVATION OF A BIOLOGICAL SAMPLE USING LIQUID CRYSTAL PHASE MODULATOR

Research Project

Project/Area Number 04555011
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 物理計測・光学
Research InstitutionSAITAMA UNIVERSITY

Principal Investigator

TOYOOKA Satoru  SAITAMA UNIVERSITY, FACULTY OF ENGINEERING, PROFESSOR, 工学部, 教授 (90019753)

Co-Investigator(Kenkyū-buntansha) NAGANO Chikara  OLYMPUS OPTICAL COL., LTD., OPTICAL RESEARCH & DEVELOPMENT DEPT., OPTICAL RESEAR, 光学技術部, 課長
KADONO Hirofumi  SAITAMA UNIVERSITY, FACULTY OF ENGINEERING, ASSOCIATE PROFESSOR, 工学部, 助教授 (70204518)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1993: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1992: ¥3,900,000 (Direct Cost: ¥3,900,000)
KeywordsPhase contrast microscope / Phase modulation device / Optical nonlinearity / Liquid crystals / Green tea extra / Phase shift method / Deconvolution / 強誘電性液晶 / 反強誘電性液晶 / 液晶位相変調素子 / 生物細胞 / ネマティック液晶
Research Abstract

Phase contrast microscope (PCM) is a key device to observe biological samples. But conventional PCM is not suited for quantitative observation. We have had an idea to quantize phase contrast image by using a variable phase shifter such as liquid crystal devices instead of a fixed phase plate. To realize the idea, following investigations have been done.
1) A common path interferometer with nematic liquid crystal (LC) phase shifter was equipped to quantize phase distribution of a 2-D phase object. Accuracy of 30/lambda was attained.
2) To observe live biological samples, high speed data access is required. Surface stabilized ferro-electric LC and antiferro-electric LC were investigated as high speed phase shifters. We found that these devices prepare required characteristics for phase shifters in PCM.
3) Nonlinear optical properties of green tea extra and resultant phase modulation properties were investigated. Full range phase modulation from 0 to 2pi is possible by changing absorbed power. Applications of this phenomena as a self-align phase filter were demonstrated.
4) A PCM made on experimental basis having an equivalent pupil plane out of the optical tube separated from the objective lens was equiped. The LC phase shifter having a ring-shape electrode corresponding to the ring slit is placed on the pupil plane. Phase quantization method based on deconvolution of a subtracted image between +pi/2 and -pi/2 phase shift images was proposed and analyzed results were demonstrated.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 豊岡了 他: "強誘電性液晶の位相変調特性(2)" 第40回応用物理学関係連合講演会予稿集III. 897 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 門野博史 他: "中国茶の光学特性に基づく自己配置位相フィルター" 第40回応用物理学関係連合講演会予稿集III. 881 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 豊岡了 他: "強誘電性液晶の位相変調特性(3)" 第54回応用物理学会講演予稿集III. 883 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 豊岡了 他: "液晶シフタを用いた位相差顕微鏡" 第54回応用物理学会講演予稿集III. 846 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 門野博史 他: "High precision common-path interferometer using a liquid crystal phase modulator." International conference:from Galileo's "Ochialinn" to optoelectronics. 895-900 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 門野博史 他: "Nonlinear optical properties of chinese tea extract" 第11回光波センセング技術研究会講演論文集. 97-104 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 豊岡了 他: "液晶位相変調器とその干渉計測への応用" 理研シンポジウム「新しい光応用技術IX」講演予稿集. 5-8 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 門野博史 他: "液晶を用いた位相シフト干渉計" 電気学会 光・量子デバイス研究会講演予稿集. 1-10 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Machida, S.Toyooka, and H.Nohira: "Phase modulation properties of ferroelectric liquid crystal cell (2)" Extended Abstracts of JSAP. 40. 879 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Kadono, R.Uma Maheswari, R.Silvenoinen, and T.Jaaskelainen: "Self-align phase filter using optical properties of chinese tea" Extended Abstracts of JSAP. 40. 881 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Machida, S.Toyooka H.Kadono and H.Nohira: "Phase modulation properties of ferroelectric liquid crystal cell (2)" Extended Abstracts of JSAP. 41. 883 (1984)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Toyooka, J.Machida H.Kadono, C.Nagano, and H.Ishiwata: "Phase-contrast microscope with liquid crystal phase modulator" Extended Abstracts of JSAP. 41. 846 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Kadono and S.Toyooka: "High precision common path interferometer using a liquid crystal phase modulator" Proc.Int.Conf.from Galileo's "Ochhialino" to optelectronics. 895-900

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] R.Uma Maheswari, H.Kadono and T.Jaaskelainen: "Nonlinear optical properties of Chinese tea extra" Proc. 9th meeting on lightwave sensing technology. 97-104 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Toyooka and H.Kadono: "Liquid crystal phase modulator and its application to interferometers" RIKEN symposium "Advances in Optical Science and Technology IX. 5-8 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 豊岡了 他: "強誘電性液晶の位相変調特性(2)" 第40回応用物理学関係連合講演会予稿集III. 897 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 門野博史 他: "中国茶の光学特性に基づく自己配置位相フィルター" 第40回応用物理学関係連合講演会予稿集III. 881 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 豊岡了 他: "強誘電性液晶の位相変調特性(3)" 第54回応用物理学会講演予稿集III. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 豊岡了 他: "液晶位相シフタを用いた位相差顕微鏡" 第54回応用物理学会講演予稿集III. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 門野博史 他: "High precision common-path interferometer using a liquid crystal phase modulator." International conference:from Galileo's "Ochialinn" to optnelectronics. 895-900 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 門野博史 他: "Nonlinear optical properties of chinese tea extract" 第11回光波センセング技術研究会講演論文集. 97-104 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 豊岡了 他: "液晶位相変調器とその干渉計測への応用" 理研シンポジウム「新しい光応用技術IX」講演予稿集. 5-8 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 門野博史 他: "液晶を用いた位相シフト干渉計" 電気学会 光・量子デバイス研究会講演予稿集. 1-10 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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