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Development of confocal microscope using random mask and active filter

Research Project

Project/Area Number 04555021
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用物理学一般(含航海学)
Research InstitutionOsaka Electro-Communication University

Principal Investigator

IKUTA Takashi  Osaka Electro-Communication University Faculty of Engineering, Professor, 工学部, 教授 (20103343)

Co-Investigator(Kenkyū-buntansha) KISHIOKA Kiyoshi  Osaka Electro-Communication University Faculty of Engineering, Professor, 工学部, 教授 (50109881)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1992: ¥5,700,000 (Direct Cost: ¥5,700,000)
KeywordsConfocal microscope / Confocal fluorescent microscope / 3-D imaging characteristics / 3-D optical transfer function / Real-time confocal microscope / Nipkow disk / Active 3-D filtering / Active image processing / ニポー円盤
Research Abstract

The present study has aimed to construct 'bright' confocal imaging system using conventional light source, under the basis of the moire fluorescent microscopy previously developed by authors.
At the first stage (1992), theoretical analysis has been carried out for the 3-dimensional optical transfer function (3D OTF) with respect to the Petran type real time fluorescent confocal microscope with a random pattern mask instead of the conventional pin hole array on the Nipkow disk. The analysis shows that mixing of the conventional image components in the 'bright' confocal image is unavoidable under the usage of the random mask pattern. Therefore, the use of the previously developed real time active 3D filter (real time 3D active convolver) is highly desirable to eliminate the conventional image components.
The development of the confocal fluorescent microscope system (based on the commercial Petran type confocal fluorescent microscope, K2-BIO) quipped the real time 3D active convolver and related control circuits has been done at the next stage (1993). Improvement of the 3D imaging characteristics have been confirmed at the usage of the 3D active convolver from present evaluation experiments, although the applied 3D weighting function is not suitable for the real time processing in this time. We have, therefore, proposed another approach for the elimination of the conventional image components, combination of a spatially split alternative mask pattern (random and uniform) for the Nipkow disk and the real time image subtraction. The possibility of this approach has been also confirmed at the present evaluation experiments.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Y.Taniguchi,T.Ikuta and R.Shimizu: "Mathematical background of defocus-modulation image processing" Optik. 94(in press). (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Taniguchi, T.Ikuta and R.Shimizu: "Mathematical background of defocus-modulation image processing" Optik. vol.94 (in press). (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Taniguchi,T.Ikuta and R.Shimizu: "Mathematical backgrowd of defocus-modulation image processing" Optik. 94(in press). (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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