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Bacteriophages as model viruses for water disinfection processes

Research Project

Project/Area Number 04555135
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 都市工学・衛生工学
Research InstitutionThe University of Tokyo

Principal Investigator

OHGAKI Shinichiro  The University of Tokyo The Faculty of Engineering Professor, 工学部, 教授 (20005549)

Co-Investigator(Kenkyū-buntansha) KAMIKO Naoyuki  The University of Tokyo The Faculty of Engineering Research Associate, 工学部, 助手 (70251345)
SUZUKI Yutaka  The University of Tokyo The Faculty of Engineering Research Associate, 工学部, 助手 (20231376)
HURUMAI Hiroaki  The University of Ibaraki The Faculty of Engineering Associate Professor, 工学部, 助教授 (40173546)
YAMAMOTO Kazuo  The University of Tokyo The Faculty of Engineering Associate Professor, 工学部, 助教授 (60143393)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥9,000,000 (Direct Cost: ¥9,000,000)
Fiscal Year 1993: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1992: ¥6,000,000 (Direct Cost: ¥6,000,000)
Keywordsbacteriophage / virus index / coliphage / RNA phage / ozonization / membrane separation / ウイルス / 水質環境 / 下水処理 / 浄化槽 / 消毒
Research Abstract

The possibility of multiplication of F-specific RNA phage (FRNA phage) mulitiplication in the environment was investigated. Using the QB strain in pure culture as a model FRNA phage, the effects of bacterial growth phage, substrate concentration and cultivation temperature on phage multiplication were studied. Similar experiments with environmental samples from raw sewage were then preformed.
Viricidal effect of ozone was investigated in an ozone demand-free batch system, using F-specific RNA coliphage QB as a model virus. Inactivation of QB did not follow the first order kinetics.
Rejection of heterotrophic and coliphages were studied by using hollow fiber membrane. Indigenous RNA phages in intake water of a water purification plant were dectected in the filtrate while DNA phages which kave larger diametes than RNA phages were not detected.
For adopting phages as model viruses for water quality control, RNA phages seems more reasonable.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] 関谷毅史,大垣眞一郎: "大腸菌ファージQβを用いたオゾンのウイルス不活化に関する研究" 水道協会雑誌. VOL.62 NO.2. 21-27 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] P.Mongkonsiri S.Ohgaki: "Modelling of Particle Associated Viruses Inactivation by Chemical Disinfection." Journal of the Faculty of Engineering. VOL.42 NO.1. 1-14 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 高橋克夫,大垣眞一郎,藤田賢二,神子直之,貝谷吉英: "中空系膜による細菌およびファージの除去特性について" 第27回日本水環境学会講演集. 234-235 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 高橋克夫,大垣眞一郎,藤田賢二,神子直之,貝谷吉英: "中空系MF膜の膜特性と細菌・ファージ除去" 第44回全国水道研究発表会講演集. 321-323 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kamiko,N.Ohgaki,S.: "Multiplication Characteristics of FRNA Phage and Its Utility as an Indicator for Pathogenic Viruses." Wat.Sci.Tech.VOL.27 NO.3-4. 133-136 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 大垣眞一郎: "ウイルス指標としてのバクテリオファージ" 水道協会雑誌. VOL.62 NO.10. 22-27 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 神子直之,大垣眞一郎: "FRNAファージの増殖特性とウイルス指標としての有効性" 水環境学会誌. VOL.16 NO.10. 723-731 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 高橋克夫,大垣眞一郎,藤田賢二,神子直之,貝谷吉英: "中空系精密濾過膜による細菌及び大腸菌ファージの除去特性" 水道協会雑誌. VOL.63 NO.4(発表予定). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 大垣眞一郎(分担執筆): "「水処理-その新しい展開」" 技報堂出版, 278 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 大垣眞一郎(分担執筆): "「分離精製技術ハンドブック」" 丸善, 1176 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 神子直之,大垣眞一郎(分担執筆): "「環境微生物工学研究法」" 技報堂出版, 417 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takeshi SEKIYA and Shinichiro OHGAKI: "Study of Inactivation of viruses by Ozone Using Coliphage QB" J.Jpn.Water Works Assoc.62(2). 21-27 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Prasang MONGKONSIRI, Shinichiro OHGAKI: "Modelling of Particle Associated Viruses Inactivation by Chemical Disinfection" J.Fac.Eng., Univ. of Tokyo. (B), 42(1). 1-14 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Katsuo TAKAHASHI, Shinichiro OHGAKI Kenji FUJITA, Naoyuki KAMIKO, Yoshihide KAIYA: "Characteristics of Removal of Bacteria and Viruses by Hollow Fiber Membrane" Proc.27th Ann.Conf., Jpn.Soc.Water Environ.234-235 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Katsuo TAKAHASHI, Shinichiro OHGAKI Kenji FUJITA, Naoyuki KAMIKO Yoshihide KAIYA: "Characteristics of Hollow Fiber MF Membrane and Rejection of Bacteria and Coliphages" Proc.44th Ann.Conf., Jpn.Water Works Assoc.321-323 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Naoyuki KAMIKO and Shinichiro OHGAKI: "Multiplication Characteristics of FRNA Phage and Its Utility as an Indicaor for Pathogenic Viruses" wat.Sci.Tech.27(3-4). 133-136 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Shinichiro OHGAKI: "Bacterio Phage as a Model Viruses" J.Jpn.Water Works Assoc.62(10). 22-27 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Shinichiro OHGAKI: "Water Treatment -Its New Development" Gihodosyuppan. 278 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Shinichiro OHGAKI: "Handbook of Separation and Purification Technology" Maruzen. 1176 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Naoyuki KAMIKO and Shinichiro OHGAKI: "Multiplication Characteristics of FRNA Phage and Utilization as an Indicator for Pathogenic Virus" J.Jpn.Soc.Water Environ.16(10). 723-731 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Katsuo TAKAHASHI, Shinichiro OHGAKI Kenji FUJITA, Naoyuki KAMIKO, Yoshihide KAIYA: "Rejection of Heterotrophic Bacteria and Coliphages by Hollow Fiber Microfiltration Membrane" J.Jpn.Water Works Assoc.63(4) (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Naoyuki KAMIKO and Shinichiro OHGAKI: "Methods for The Study of Environmental Microbiology" Gihodosyuppan. 417 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 高橋克夫、大垣眞一郎、藤田賢二、神子直之、貝谷吉英: "中空系MF膜の膜特性と細菌・ファージ除去" 第44回全国水道研究発表会講演集. 321-323 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kamiko,N. Ohgaki,S.: "Multiplication Characteristics of FRNA Phage and Its Utility as an Indicator for Pathogenic Viruses." Wat.Sci.Tech.VOL.27, NO.3-4. 133-136 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 大垣眞一郎: "ウイルス指標としてのバクテリオファージ" 水道協会雑誌. VOL.62, NO.10. 22-27 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 神子直之,大垣眞一郎: "FRNAファージの増殖特性とウイルス指標としての有効性" 水環境学会誌. VOL.16, NO.10. 723-731 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 高橋克夫,大垣眞一郎,藤田賢二,神子直之,貝谷吉英: "中空系精密濾過膜による細菌及び大腸菌ファージの除去特性" 水道協会雑誌. VOL.63, NO.4, (発表予定). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 神子直之,大垣眞一郎(分担執筆): "環境微生物工学研究法" 技報堂出版, 417 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 関谷 毅史: "大腸菌ファージQβを用いたオゾンのウイルス不活化に関する研究" 水道協会雑誌. 62. 21-27 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 浦瀬 太郎: "水処理用分離膜の細孔径分布の測定とウイルス阻止率" 第27回日本水環境学会年会講演集. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 高橋 克夫: "中空糸膜による細菌およびファージの除去特性について" 第27回日本水環境学会年会講演集. (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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