| Project/Area Number |
04555225
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
化学工学
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
FUNATSU Kazumori Kyushu Univ. Dept.Chem.Eng. Prof., 工学部, 教授 (80037960)
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| Co-Investigator(Kenkyū-buntansha) |
OMOTO Mitsuru INOAC Technical Center R&D Manager, 主任研究員
MATSUSHITA Taku Kyushu Univ. Dept.Chem.Eng. Res.Assoc., 工学部, 助手 (10209538)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥16,200,000 (Direct Cost: ¥16,200,000)
Fiscal Year 1993: ¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 1992: ¥8,900,000 (Direct Cost: ¥8,900,000)
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| Keywords | Polyurethane foam / Hepatocyte Spheroid / Three dimensional culture / Multi-Capillary PUF packed-bed / Hepatic failure rat / Ammonia detoxification / Extracorporeal circulation system / Hybria Artificial liver / 正常肝細胞 / 多細管型PUF充填層 / 生体外組織構築 / プロテオグリカン / 細胞分化機能発現 |
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| Research Abstract |
1. We designed a prototype of hybrid artificial liver module using optimum polyurethane foam (PUF) material for spheroid formation of hepatocyte in vitro. The module was type of packed-bed with PUF plates (1.5X40X40mm) which immobilized spheroids. In serum-free medium supplemented with 1mM NH_4Cl as a toxic substance, this module detoxified ammonia and synthesized urea from ammonia. The urea synthesis rate of hepatocyte in the module was slightly higher than that of hepatocyte in vivo and was maintained for about 3 weeks.
2. We also developed Multi-Capillary PUF packed-bed for increasing a cell density and achieving an efficient mass transfer, in which a cylindrical PUF block with many capillaries for medium flow was packed. Consequently, a high cell density of 1X10^7cells/cm^3-PUF was achieved and hepatocyte/spheroids were formed in the module. The urea synthesis rate of the module was 10 times higher than that of prototype module.
3. A volume of the hybrid artificial liver module containing 1/10 amount of human liver hepatocytes was estimated to be about 2 liter by using the Multi-Capillary PUF packed-bed. This volume seemed to be a medically applicable value.
4. We developed an extracorporeal circulation system for acute hepatic failure rat to investigate the performance of the artificial liver module in ex vivo experiment. The ammonia concentration in blood increased by actute failure was gone down to normal level by the artificial liver for 3 hour circulation and the hepatic failure rat was recovered.
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