| Project/Area Number |
04556009
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用生物化学・栄養化学
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| Research Institution | Nagoya University |
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Principal Investigator |
KITAGAWA Yasuo Nagoya University Bioscience Center, Professor, 生物分子応答研究センター, 教授 (50101168)
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| Co-Investigator(Kenkyū-buntansha) |
MIKI Kiyoshi Nagoya University Bioscience Center, Research Associate, 生物分子応答研究センター, 助手 (30212228)
UEDA Masatugu Snow-Brand Milk Co.Ltd.Director, 生物科学研究所, 主査
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥20,600,000 (Direct Cost: ¥20,600,000)
Fiscal Year 1993: ¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1992: ¥14,500,000 (Direct Cost: ¥14,500,000)
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| Keywords | Erythropoietin / Polarized secretion / Polymer medicine / Glycen-processing / 糖鎖修飾 / 坡構造 / 表裏極性分泌 / 中空系 / 動物細胞工学 |
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| Research Abstract |
Polarized epithelial cells are characterized by two plasma membrane domains, apical and basolateral domains, having different structure and functions. The polarity of epithelial cells is not only restricted to plasma membrane components but secretory proteins are also released in a polarized fashion. MDCK cells are a typical epithelial cell line derived from dog kidney and have been widely used to investigate molecular mechanism of vectorial transport of membrane lipids and proteins. Erythropoietin (Epo) is a major physiological regulator of erythropoiesis by stimulating proliferation and differentiation of erythroid precursor cells. We here found that recombinant human Epo is preferentially secreted from the apical domain of MDCK cells. Taking advantage of this polarized secretion, we have developed a pilot system of producing Epo in industrial scale. We have collected basic information of deigning hollow-fiber system in which Epo will be released either inside or outside of the fiber.
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