Preparation of cellulose sulfonic acids and examination of their anti-Rous sarcoma virus activity
| Project/Area Number |
04556022
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
林産学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ISHIZU Atsushi The University of Tokyo Faculty of Agriculture, Emeritus Professor, 農学部, 名誉教授 (40014922)
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| Co-Investigator(Kenkyū-buntansha) |
YASHIRO Makoto Nippon Paper Company Research Laboratory of Products Development, Senior researc, 商品開発研究所, 主席研究員
MESHITSUKA Gyousuke The University of Tokyo Faculty of Agriculture, Professor, 農学部, 教授 (30012074)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1993: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1992: ¥4,300,000 (Direct Cost: ¥4,300,000)
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| Keywords | cellulose / sulfonic acid / virus / Rous sarcoma virus / AIDS / Human immuno-deficiency virus / 鶏肉腫 / ウィルス |
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| Research Abstract |
The preparation of cellulose sulfonic acids was attempted by four methods : 1) etherification of cellulose or cellulose acetate with sodium 2-bromoethyl sulfonate, 2) etherification of cellulose or cellulose acetate with propanesultone, 3) addition of sodium hydrogen sulfite to allylcellulose, 4) nucleophilic reaction of hydrogen sulfite ion with tosylates of cellulose and hydroxyethyl-cellulose. The preparations by the methods 1 and 2 were carried out heterogeneously in an aqueous iso-propanol and homogeneously in organic solvents. Among these methods only the method 2 gave the products having high degrees of substitution (DS) : CPS-1 with DS 2.11 from cellulose acetate in dimethylsulfoxide by one step and CPS-2 with DS 1.93 from cellulose by three times of the treatment in the aqueous solvent.
Sulfonic acids of curdlan (DS 1.21) and of dextran (DS 0.69) also were prepared by the method 2 in the aqueous solvent.
Cellulose sulfonic acids prepared by the methods 1 and 2 as well as the sulfonic acids of curdlan and of dextran were subjected to anti-HIV test. The anti-HIV activity measured by an improved MTT method was found to be significant only for CPS-1 and -2, which have high DSs by sulfonic acid group. The activity for CPS-1 was comparable to that for dideoxyinosine, which is admitted to use as a medicine for AIDS, but was inferior to dextran sulfate which exerts the activity according to the same mechanism as CPS.CPS-1 was inferior to dextran sulfate also in its ability to inhibit the formation of syncytia.
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Report
(3 results)
Research Products
(4 results)
