| Project/Area Number |
04557002
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
神経解剖学
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| Research Institution | Osaka University |
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Principal Investigator |
TOHYAMA Masaya Osaka University, Professor, 医学部, 教授 (40028593)
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| Co-Investigator(Kenkyū-buntansha) |
SHIOSAKA Sadao Nara Institute of Science & Technology, Professor, バイオサイエンス科, 教授 (90127233)
WANAKA Akio Osaka University, Associate Prof., 医学部, 助教授 (90210989)
KIYAMA Hiroshi Osaka University, Associate Prof., 医学部, 助教授 (00192021)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥8,600,000 (Direct Cost: ¥8,600,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1993: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥5,700,000 (Direct Cost: ¥5,700,000)
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| Keywords | transcription factor / DNA binding protein / nuclear transport signal / gene transfer / liposome / histochemistry / nervous system / 転写抑制 / 細胞内遺伝子導入 / HVJ-リポゾーム / 二本鎖DNAプローブ / HMG1 / SV40T抗原 / 発現抑制 |
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| Research Abstract |
We investigated following projects
1 : Visualization of DNA binding protein in situ by using double strand DNA fragment.
2 : An attempt for inhibition of transcription factors'DNA binding by using double strand DNA fragment.
For the project3 #1, we have succeeded in establishing the method. We used ^<35>S labeled double strand DNA fragment (Ca.20 base par) which has a AP-1 or CRE motif for the detection of binding protein. The positive signal was observed in cell nucleus.
For the project #2, we developed a method which allowed the efficient transportation of DNA fragment into cell nucleus. This was achieved by conjugating nuclear transport signal peptide from SV40 Large T antigen and wrapping by liposome.
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