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Visualization of DNA binding protein in situ by using double strand DNA fragment, and an attempt of inhibition of the transcription factors' DNA binding by using double strand DNA fragment.

Research Project

Project/Area Number 04557002
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 神経解剖学
Research InstitutionOsaka University

Principal Investigator

TOHYAMA Masaya  Osaka University, Professor, 医学部, 教授 (40028593)

Co-Investigator(Kenkyū-buntansha) SHIOSAKA Sadao  Nara Institute of Science & Technology, Professor, バイオサイエンス科, 教授 (90127233)
WANAKA Akio  Osaka University, Associate Prof., 医学部, 助教授 (90210989)
KIYAMA Hiroshi  Osaka University, Associate Prof., 医学部, 助教授 (00192021)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥8,600,000 (Direct Cost: ¥8,600,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1993: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥5,700,000 (Direct Cost: ¥5,700,000)
Keywordstranscription factor / DNA binding protein / nuclear transport signal / gene transfer / liposome / histochemistry / nervous system / 転写抑制 / 細胞内遺伝子導入 / HVJ-リポゾーム / 二本鎖DNAプローブ / HMG1 / SV40T抗原 / 発現抑制
Research Abstract

We investigated following projects
1 : Visualization of DNA binding protein in situ by using double strand DNA fragment.
2 : An attempt for inhibition of transcription factors'DNA binding by using double strand DNA fragment.
For the project3 #1, we have succeeded in establishing the method. We used ^<35>S labeled double strand DNA fragment (Ca.20 base par) which has a AP-1 or CRE motif for the detection of binding protein. The positive signal was observed in cell nucleus.
For the project #2, we developed a method which allowed the efficient transportation of DNA fragment into cell nucleus. This was achieved by conjugating nuclear transport signal peptide from SV40 Large T antigen and wrapping by liposome.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Kiyama,H.: "Recent Progress in the use of the techniques molecular histochemistry." Jap. J. Thoracic Diseases. 31. 100-107 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Koto, K.: "Gene transfer and the expressi on of a foreign gene in vivo in post-mitotic neurons of the adult rat brain using the hemagglutinating virus of the Japan-liposome methd" Mol. Brain Res.25. 359-363 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kiyama, H.: "Recent Progress in molecular histochemistry." Acta Anat. Nippon. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kiyama H: "Recent Progress in the use of the techniques molecular histochemistry" Jap.J.Thoracic Diseases. 31. 100-107 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kato K,et al.: "Gene transfer and the expression of a foreign gene in vivo in post-mitotic neurons of the adult rat brain using the hemagglutinating virus of the Japan-liposome methd" Mol.Brain Res.25. 359-363 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kiyama H: "Recent progress in molecular histochemistry" Acta Anat.Nippon. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kiyama,H.: "Recent Progress in the use of the techniques molecular histochemistry" Jap.J.Thoracic Diseases. 31. 100-107 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kato,K.: "Gene transfer and the expression of a goreign gene in vivo in post-mitotic neurons of the adult rat brain using the hemagglutinating virus of the Japan-liposome methd." Mol.Brain Res.25. 359-363 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] kiyama,H.: "Recent progress in molecular histochemistry." Acta Anat.Nippon. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] H,Kiyama: "Sympathetic and parasympathetic ganglia express non-NMDA type glutamate receptors:distinct receptor subunit composition in the principle and SIF cells" Molecular Brain Research. 19. 345-348 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Kiyama: "Characteristic localization of non-NMDA type glutamate receptor subunits in the rat pituitary gland" Molecular Brain Research. 19. 262-268 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Furuyama: "Region-specific expression of subunits of ionotropic glutamate receptors(AMPA-type,KA-type and NMDA receptors)in the rat spinal cord with special reference to nooicopption" Molecular Brain Research. 18. 141-151 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Sato: "AMPA,KA and NMDA receptors are expressed in the rat DRG neurones" Neuroreport. 4. 1263-1265 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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