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Development of a model system for somatic cell gene therapy using hematopoietic stem cells and lymphoid cells.

Research Project

Project/Area Number 04557034
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionUniversity of Tsukuba (1994)
0082401 (1992-1993)

Principal Investigator

NAKAUCHI Hiromitsu (1992, 1994)  Univ.of Tsukuba Dept.of Immunology, Professor, 基礎医学系, 教授 (40175485)

中村 幸夫 (1993)  理化学研究所, 造血制御研究チーム, 研究員 (60231479)

Co-Investigator(Kenkyū-buntansha) TANI Kenzaburo  Institute of Medical Science Univ.of Tokyo, Assitant Professor, 医科学研究所, 講師 (00183864)
HIROCHIKA Rei  RIKEN Lab.of Cell Growth and Differentiation, Staff Scientist, 造血制御研究チーム, 研究員 (90260223)
NAKAMURA Yukiko  Univ.of Tsukuba Dept.of Immunology, Assitant Professor, 基礎医学系, 講師 (60231479)
徳元 康人  筑波大学, 基礎医学系, 助手 (70261170)
中内 啓光  筑波大学, 基礎医学系・免疫学, 教授 (40175485)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥15,800,000 (Direct Cost: ¥15,800,000)
Fiscal Year 1994: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1993: ¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1992: ¥7,200,000 (Direct Cost: ¥7,200,000)
Keywordssomatic cell gene therapy / hematopoietic stem cells / cytokines / diabetes / bone marrow transplantation / CD34 / 多能性 / 自己複製能 / c-kit / 免疫寛容 / clonal deletion / clonal anergy / 骨髄キメラ / 遺伝子治療 / 血液幹細胞 / インシュリン / アガロース
Research Abstract

1)Establishment of a mouse model system of a somatic cell gene therapy with an immunological safety system.
Mouse fibroblasts were transfected with an expression vector containing human insulin cDNA.When one million of these fibroblasts were injected i.p.into streptozocin-induced diabetic mice, we observed a significant improvement of the blood glucose concentrations. To ensure the safety of somatic cell gene therapy, the proinsulin-producing fibroblasts were further transfected with an expression vector containing CD8.2, a gene encoding a cell surface antigen.Somatic gene therapy with these doubly transfected cells followed by the administration of a monoclonal antibody to CD8.2 resulted in an initial decrease of blood glucose concentrations followed by the permanent recurrence of hyperglycemia, thus proving the complete removal of the transplanted cells.
2)Purification of the hematopoietic stem cells in adult mouse bone marrow.
Using monoclonal antibodies and FACS,we established a system by which the hematopoietic stem cells can be isolated. The results indicate that c-kit+Sca-1+CD34-Lin-cells constituting only 0.008% of bone marrow mononuclear cells are highly enriched for long-term marrow repopulating ability. We were able to reconstitute bone marrow of a lethally irradiated mouse by an injection of a single c-kit+Sca-1+CD34-Lin-cell. We also demonstrated that short-term culture of enriched stem cells under certain cytokines increase the efficiency of retroviral transdution into the hematopoietic stem cells.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (80 results)

All Other

All Publications (80 results)

  • [Publications] Yasushi Kawakami: "Somatic gene therapy for diabetes with an immunological safety system for complete removal of transplanted cells." Diabetes.41. 956-901 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yumi Matzuzaki: "Evidence for the existence of two parallel differentiation pathways in the thymus of MRL lpr/lpr mice." J. Immunol.149. 1069-1074 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K. Muroi: "Reactivity profiles of leukemic myeloblasts with monoclonal antibodies directed to sialosyl-Le(x)and other lacto-series type 2 chain antigens:absence of reactivity with normal hematopoietic progenitor cells." Blood.79. 713-719 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yukio Nakamura: "A truncated erythropoietin receptor that fails to prevent programmed cell death of erythroid cells." Science.257. 1138-1141 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yuji Gunji: "Expression and function of adhesion molecules on human hemopoietic stem cells:CD34+LFA-1-cells are more primitive than CD34+LFA-1+cells." Blood.80. 429-436 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Seiji Okada: "Synergistic effect of IL-3 and IL-6 on highly enriched murine hemopoietic progenitors." Exp.Hematology.20. 546-551 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hideki Taniguchi: "Treatment of diabetic mice with the encapsulated fibroblasts producing human proinsulin." Trans.Proceedings.24. 2977-2978 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Seiji Okada: "In vivo and in vitro stem cell function of c-kit and Sca-1-positive murine hemopoietic cells." Blood.80. 3044-3050 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yukiko Nakamura: "Two alternative forms of cDNA encoding CD34." Exp. Hematology.21. 236-242 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mitsuo Nishikawa: "Changes in hematopoiesis-supporting ability of C3H10T1/2 mouse embryo fibroblasts during differentiation." Blood.81. 1184-1192 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Akira Shibuya: "Enrichment of Interleukin-2-responsive Natural Killer progenitors in human bone marrow." Blood.81. 1819-1826 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Seiji Okada: "Sequential analysis of hematopoietic reconstitution achieved by transplantation of hamatopoietic stem cells." Blood.81. 1720-1725 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kei-ichi Nakayama: "Cyclosporin A inhibits the decrease of CD4/CD8 expression induced by protein kinase C activation." Int.Immunol.5. 419-426 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mayumi Onishi: "CD4 Dull Positive Hematopoietic Stem Cells in Murine bone marrow." Blood.81. 3217-3225 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yumi Matsuzaki: "Characterization of c-kit positive intrathymic stem cells that are restricted to lymphoid differentiation." J. Exp. Med.178. 1283-1292 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yuji Gunji: "Human primitive hematopoietic progenitor cells are more enriched in KITlow cells than in KIT high cells." Blood.82. 3283-3289 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hideki Taniguchi: "Use of cytokines for efficient introduction of foreign genes into the hematopoietic stem cell." Transplant.Proc.26. 1972-1974 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hideki Taniguchi: "Hematopoietic stem cell chimerism:Relationships between degree of chimerism,T cell clonal deletion and graft survival." Transplant.Proc.26. 1966-1968 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kei-ichi Nakayama: "CD8 b -chain is necessary for positive selecion of CD8 T cells." Science. 263. 1131-1133 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Keiko Nohara: "Glycosphingolipids of rat T cells.Predominance of Asialo-GM1 and GD1c." Biochemistry.33. 4661-4666 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yukio Nakamura: "A truncated erythorpoietin receptor and cell death:a reanalysis." Science. 264. 588-589 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Tani: "Retrovirus-mediated gene transfer of human pyruvate kinase(PK)cDNA into murine hematopoietic cells:Implications for gene therapy of human PK deficiency." Blood.83. 2305-2310 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kazuhiko Igarashi: "Structure,expression and function of small Maf family protein MafK in mouse." J. Biol. Chem.( in press ).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hideki Taniguchi: "Evidence for the presence of hematopoietic stem cells in the adult liver." Transplant.Proc.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Akira Shibuya: "Lymphokine requirement for the generation of NK cells from CD34+ Hematopoietic Progenitor Cells." Blood. ( in press ).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 中内啓光: "代謝.29:臨時増刊号 免疫92'血液幹細胞の純化と増殖因子" 中山書店, 10 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 中村幸夫: ""アポトーシスを介した赤芽球系細胞の増殖分化制御機構"実験医学増刊号「アポトーシス-細胞死の機構-」," 羊土社, 6 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 松崎有未: ""ソーティングの実際と注意点",フローサイトメトリー入門" 日本 サイトメトリー学会, 4 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 中村光三: ""セルソーター",日本生化学会編新-生化学実験講座20-機器分析概論" 東京化学同人, 12 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasushi Kawakami, et al.: "Somatic gene therapy for diabetes with an immunological safety system for complete removal of transplanted cells." Diabetes. 41. 956-961 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yumi Matzuzaki, et al.: "Evidence for the existence of two paralled differentiation pathways in the thymus of MRL lpr/lpr mice." J.Immunol. 149. 1069-1074 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Muroi, K., et al.: "Reactivity profiles of leukemic myeloblasts with monoclonal antibodies directed to sialosyl-Le (x) and other lacto-series type 2 chain antigens : absence of reactivity with normal hematopoietic progenitor cells." Blood. 79. 713-719 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yukio Nakamura, Norio Komatsu, Nakauchi Hiromitsu: "A truncated erythropoietin receptor that fails to prevent programd cell death of erythroid cells." Science. 257. 1138-1141 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yuji Gunji, et al.: "Expression and function of adhesion molecules on human hemopoietic stem cells : CD34+LFA-1-cells are more primitive than CD34+LFA-1+cells." Blood. 80. 429-436 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Seiji Okada.et al.: "Synergistic effect of IL-3 and IL-6 on highly enriched murine hemopoietic progenitors." Exp.Hematology. 20. 546-551 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hideki Taniguchi, et al.: "Treatment of diabetic mice with the encapsulated fibroblasts producing human proinsulin." Trans.Proceedings. 24. 2977-2978 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Seiji Okada, Hiromitsu Nakauchi, Kazunari Nagayoshi, Shinichi Nishikawa, Yasusada Miura and Toshio Suda: "In vivo and in vitro stem cell function of c-kit and Sca-1-positive murine hemopoietic cells." Blood. 80. 3044-3050 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yukio Nakamura, Hajime Komano and Hiromitsu Nakauchi: "Two alternative forms of cDNA encoding CD34." Exp.Hematology. 21. 236-242 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yukio Nakamura and Hiromitsu Nakauchi: "A Truncated erythropoietin receptor and cell death.[technical comments]" Science. 259. 696 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mitsuo Nishikawa, et al.: "Changes in hematopoiesis-supporting ability of C3H10T1/2 mouse embryo fibroblasts during differentiation." Blood. 81. 1184-1192 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Akira Shibuya, et al.: "Enrichment of Interleukin-2-responsive Natural Killer progenitors in human bone marrow." Blood. 81. 1819-1826 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Seiji Okada, et al.: "Sequential analysis of hematopoietic reconstitution achieved by transplantation of hamatopoietic stem cells." Blood. 81. 1720-1725 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kei-ichi Nakayama and Hiromitsu Nakauchi: "Cyclosporin A inhibits the decrease of CD4/CD8 expression induced by protein kinase C activation." Int.Immunol. 5. 419-426 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiromitsu Nakauchi et Gabriel Gachelin: "Les cellules souches du sang." La Recherche. 254. 537-541 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mayumi Onishi, et al.: "CD4 Dull Positive Hematopoietic Stem Cells in Murine bone marrow." Blood. 81. 3217-3225 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiromitsu Nakauchi et Gabriel Gachelin: "Las cellulas madre de la sangre." Mundo Cientifico. 137. 619-623 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yumi Matsuzaki, et al.: "Characterization of c-kit positive intrathymic stem cells that are restricted to lymphoid differentiation." J.Exp.Med. 178. 1283-1292 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yuji Gunji, et al.: "Human primitive hematopoietic progenitor cells are more enriched in KITlow cells than in KIT high cells." Blood. 82. 3283-3289 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hideki Taniguchi, et al.: "Use of cytokines for efficient introduction of foreign genes into the hematopoietic stem cell." Transplant.Proc.26. 1972-1974 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hideki Taniguchi, et al.: "Hematopoietic stem cell chimerism : Relationships between degree of chimerism, T cell clonal deletion and graft survival." Transplant.Proc.26. 1966-1968 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kei-ichi Nakayama, et al.: "CD8 b-chain is necessary for positive selecion of CD8 T cells." Science. 263. 1131-1133 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Keiko Nohara, Hiromitsu Nakauchi, and Sarah Spiegel: "Glycosphingolipids of rat T cells.Predominance of Asialo-GM1 and GD1c." Biochemistry. 33. 4661-4666 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yukio Nakamura and Hiromitsu Nakauchi: "A truncated erythorpoietin receptor and cell death : a reanalysis." Science. 264. 588-589 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Tani, et al.: "Retrovirus-mediated gene transfer of human pyruvate kinase (PK) cDNA into murine hematopoietic cells : Implications for gene therapy of human PK deficiency." Blood. 83. 2305-2310 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kazushi Inoue, et al.: "WT1 as a new prognostic factor and a new marker for the detection of minimal residual disease in acute leukemia." Blood. 84. 3071-3079 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Eiji Kajii, et al.: "Expression of Rh blood group gene transcripts in human leukocytes." Biochem.Biophys.Res.Commun. 202. 1497-1504 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kazuhiko Igarashi, et al.: "Structure, expression and function of small Maf family protein MafK in mouse." J.Biol.Chem. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hideki Taniguchi et al.: "Evidence for the presence of hematopoietic stem cells in the adult liver." Transplant.Proc.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Akira Shibuya, et al.: "Lymphokine requirement for the generation of NK cells from CD34+ Hematopoietic Progenitor Cells." Blood. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hideki Taniguchi: "Use of cytokines for efficient introduction of foreign genes into the hematopoietid stem cell." Transplant Proc.26. 1972-1974 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hideki Taniguchi: "Hamatopoietic stem cell chimerism: Relationships between degree of chimerism,T cell clonal deletion and graft survival." Transplant Proc.26. 1966-1968 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Tani: "Retrovirus-mediated gene transfer of human pyruvate kinase(PK)cDNA into murine hematopoietic cells: Implications for gene therapy of human PK deficiency." Blood.83. 2305-3210 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kazuhiko Igarashi: "Structure,expression and function of small Maf family protein MafK in mouse." J.Biol.Chem.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Hideki Taniguchi: "Evidence for the presence of hematopoietic stem cells in the liver." Transplant Proc.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Akira Shibuya: "Lymphokine requirement for the generation of NK cells from CD34+Hematopoietic Progenitor Cells." Blood. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Mayumi Onishi,et.al.: "CD4 Dull Positive Hematopoietic Stem Cells in Murine bone marrow." Blood.81. 3217-3225 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yumi Matsuzaki,et.al.: "Characterization of c-kit positive intrathymic stem cells that are restricted to lymphoid differentiation." J.Exp.Med.178. 1283-1292 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yuji Gunji,et.al.: "Human primitive hematopoietic progenitor cells are more enriched in KITlow cells than in KIT high cells." Blood.82. 3283-3289 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hideki Taniguchi,et.al.: "Hematopoietic stem cell chimerism:Relationships between degree of chimerism,T cell clonal deletion and graft survival." Transplant.Proc.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Hideki Taniguchi,et.al.: "Use of cytokines for efficient introduction of foreign genes into the hematopoietic stem cell." Transplant.Proc.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] 谷口 英樹、中内 啓光: "Gene Targetingを用いた遺伝子治療の可能性." 病態生理.11. 782-787 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 中内 啓光: "遺伝子治療の現状と今後の展開." 臨床分子医学.1. 65-72 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yasushi Kawakami,Hiromitsu Nakauchi et al.: "Somatic gene therapy for diabetes with an immunological safety system for complete removal of transplanted cells." Diabetes.41. 956-961 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yukio Nakamura,Nakauchi Hiromitsu et al.: "A truncated erythropoietin receptor that fails to prevent programmed cell death of erythroid cells." Science.257. 1138-1141 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yuji Gunji,Hiromitsu Nakauchi et al.: "Expression and function of adhesion molecules on human hemopoietic stem cells:CD34^+LFA-1^- cells are more primitive than CD34^+LFA-1^+ cells." Blood.80. 429-436 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Seiji Okada,Hiromitsu Nakauchi et al.: "Synergistic effect of IL-3 and IL-6 on highly enriched murine hemopoietic progenitors." Exp.Hematology.20. 546-551 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hideki Taniguchi,Hiromitsu Nakauchi et al.: "Treatment of diabetic mice with the encapsulated fibroblasts producing human proinsulin." Trans.Proceedings.24. 2977-2978 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Seiji Okada,Hiromitsu Nakauchi et al.: "In vivo and in vitro stem cell function of c-kit and Sca-1-positive murine hemopoietic cells." Blood.80. 3044-3050 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yukio Nakamura,Hiromitsu Nakauchi et al.: "Two alternative forms of cDNA encoding CD34." Exp.hematology.21. 236-242 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Mitsuo Nishikawa,Hiromitsu Nakauchi et al.: "Changes in hematopoiesis-supporting ability of C3H10T1/2 mouse embryo fibroblasts during differentiation." Blood.

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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