• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

DEVELOPMENT OF A NEW PROGRAM OF IVF-ET BY THE USE OF NOVEL CULTURE CONDITIONS AND NEW METHOD OF EMBRYO TRANSFER

Research Project

Project/Area Number 04557073
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionSHIGA UNIVERSITY OF MEDICAL SCIENCE,FACULTY OF MEDICINE, (1993-1994)
Kyoto University (1992)

Principal Investigator

NODA Yoichi  SHIGA UNOVERSITY OF MED SCI,DEPARTMENT OF OBSTETRICS AND GYNECOLOGY,PROFESSOR,, 医学部, 教授 (50115911)

Co-Investigator(Kenkyū-buntansha) GOTO Yasuo  KYOTO UNIVERSITY,FACULTY OF MEDICINE,ASSISTANT PROFESSOR,, 医学部, 助手 (70243029)
YAMAMOTO Yoshiaki  SHIGA UNIVERSITY OF MED SCI,ASSISTANT PROFESSOR,, 医学部, 助手 (60220505)
馬岡 陽  京都大学, 医学部, 助手 (80223593)
成本 勝彦  京都大学, 医学部, 助手 (70228097)
森 崇英  京都大学, 医学部, 教授 (90026865)
入谷 明  京都大学, 農学部, 教授 (80026385)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥17,000,000 (Direct Cost: ¥17,000,000)
Fiscal Year 1994: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1993: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1992: ¥8,600,000 (Direct Cost: ¥8,600,000)
KeywordsLOW OXYGEN CULTURE, / LOW ILLUMINATION CULTURE, / INTRAENDOMETRIAL EMBRYO REPLACEMENT, / 着床 / 胚移植法 / 胚発生停止 / マウス胚培養 / ヒト体外受精・胚移植法 / 胚移植カテーテル / 酸化的ストレス
Research Abstract

At the initial phase of this investigation, promoting effects of superoxide dismutase on mouse embryo development in vitro was found. Starting from this simple experimantal results, a variety of agents which posess anti-oxidation activity were tested for the promoting effects using mouse embryo culture system. After thorough investigations, a concepts-oxidative stress- was introduced into the embryo culture. When the embryos were cultured under the low oxygen conditions or under the low illuminations, a higher blastulation rate was obtained in the mouse embryos. This promoting effect was also observed on hamster embryos as well as rats embryos. Thus the new concept was found to be applicable over species, human embryo culture was desined to carriey out under the same conditions. A remarkably higher blastulation rate of 60% was obtained when cultured under low illumination, low oxygen and R-MEM medium which had been known as the culture medium with minimum adverse effect on embryos with respect to oxidative stress. Previously, blastulation rate of human embryos was known at aroud 18% as reported by Cambridge group. By the another line of experiment, intraendometrial transfer of the mouse embryo was found to be feasible for testing embryo viability as well as endometrial integrity. This simple experimental result indicated that in the course of implantation, apposition as well as penetration of trophoblast into endometrial stromal space are not indispensable for establoshing the implantation. At the end of investigation period, a new model for tranferring the human embryos into the endometrial stroma was invented, but application of this method was not completed so far. Efficacy of this method still remains to be evaluated.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Masahide Shiotani, Yoichi Noda 他: "Endometriosis of the Groin:A Casre Report" Jpn. J. Fertil. Steril.39. 6-9 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takashi Tokura, Yoichi Noda 他: "Sequential observation of mitochondrial distribution in mouse oocytes and embryos" J Assisted Reproduction Genetics. 10. 417-426 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Junji KISI, Yoichi NODA 他: "Analysis of In Vitro Developmental Block of Rat Embryos‐‐‐Assessment from the View Point of Oxygen Toxicity‐‐‐" Journal of Reproduction and Development. , in press.(1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takahiro NAKAYAMA, Yoichi NODA 他: "Effects of Hochu-Ekki-To (TJ41) a JapaneseKampo medicine, on cultured hamster epididymal cells" Am. J. Chinese. 301-307 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takahiro NAKAYAMA, Yoichi NODA 他: "Accerelating Effects of Kampo medicines,on protein synthesis in cultured hamster epididymal cells" Japan Journal of Fertility and Sterility. in press. (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takafumi Nonogaki, Yoichi Noda 他: "Developmental Blockage of Mouse embryos Caused by Fatty Acids" J. Assist. Reprod. Genet. : accepted for publication. (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoich Noda, Yasuo Goto 他: "Cysteine as a embryonic growth stomulator factor in co-culture with oviductal cell" In:Perspectives in Assisted Reproduction, Frontees in Endocrinology vol4. Ares-Serono, (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasio GOTO, Yoichi Noda 他: "Effect of superoxide dismutase on mouse embryos in vitro" In Fronties of reactive oxygen species in biology and medicine., (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Masahide Shiotani, Yoichi Noda: Endometriosis of the Groin : A Casre Report Jpn.J.Fertil.Steril.39. 6-9 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takasi ToKura, Yoichi Noda: Sequential observation of mitochondrial distribution in mouse oocytes and embryos J Assisted Reproduction Genetics. 10. 417-426 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Junji KISI,Yoichi NODA: Analysis of In Vitro Developmental Block of Rat Embryos -Assessment from the View Point of Oxygen Toxicity-Journal of Reproduction and Development.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takahiro NAKAYAMA,Yoichi NODA: Effects of Hochu-Ekki-To (TJ41) a Japanese Kampo medicine, on cultured hamster epididymal cells Am. J.Chinese medicine. 301-307 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takahiro NAKAYAMA,Yoichi NODA: Accerelating Effects of Kampo medicines, on protein synthesis in cultured hamster epididymal cells Japan Journal of Fertility and Sterility. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takafumi Nonogaki, Yoichi Noda: Developmental Blockage of Mouse embryos Caused by Fatty Acids J.Assist. Reprod.Genet.(accepted for publication). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoichi Noda, Yasuo GOTO: Cysteine as a embryonic growth stomulator factor in co-culture with oviductal cell In : Perspectives in Assisted Reproduction, Frontees in Endocrinology vo14. Ares-Serono. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasuo GOTO,Yoichi Noda: Effect of superoxide dismutase on mouse embryos in vitro In Fronties of reactive oxygen species in biology and medicine.(1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Masahide Shiotani,Yoichi Noda,他: "Endometriosis of the Groin:A Casre Report" Jpn.J.Ferti.Steril.39. 6-9 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takasi Tokura,Yoichi Noda,他: "Sequential observation of mitochondrial distribution in mouse oocytes and embryos" J Assisted Reproduction Genetics. 10. 417-426 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Junji KISI,Yoichi NODA,他: "Analysis of In Vitro Developmental Block of Rat Embryos…Assessment from the View Point of Oxyfgen Toxicity…" Journal of Reproduction and Development.(in press). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takahiro NAKAYAMA,Yoichi NODA,他: "Effects of Hochu-Ekki-To(TJ41)a Japanese Kampo medicine,on cultured hamster epididymal cells" Am.J.Chinese medicine. 301-307 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takahiro NAKAYAMA,Yoichi NODA,他: "Accerelating Effects of Kampo medicines,on protein synthesis in cultured hamster epididymal cells" Japan Journal of Fertility and Sterility.(in press). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takafumi Nonogaki,Yoichi Noda,他: "Developmental Blockage of Mouse embryos Caused by Fatty Acids" J.Assist.Reprod.Genet.(accepted for publication). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yoichi Noda,Yasuo GOTO,他: "Cysteins as a embryonic growth stomulator factor in co-culture with oviductal cell" In:Perspectioves in Assisted Reproduction,Frontees in Endocrinology vo14.Ares-Serono, (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yasuo GOTO,Yoichi Noda,他: "Effect of superoxide dismutase on mouse embryos in vitro" In Fronties of reactive oxygen species in biology and medicine., (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Satoshi Natsuyama,Yoichi NODA 他: "Role of Protein Supplements in the Culture of Mouse Embryos" Theriogenology. 40. 149-157 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Masahide Shiotani,Yoichi NODA 他: "Immunohistochemical Localization of CuZn-Superoxide Dismutase in Human Cervix and Cervical Cancer" Acta Histochemica et Cytochemica. 126. 57-64 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Goto,Y.: "Oxidative stress on mouse embryo development in vitro" Free Radical Biology & Medicine. 13. 47-53 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hayashi,S.: "Effects of Anti-Swine Zona Serum on Swine and Murine in Vitro fertilization" Int.J.Fertility.36. 307-316 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Natsuyama,S.: "Release of mouse two-cell block by reducfion of protein disulfide with thioredoxin from E.celi." J.Reprod.Fertil.95. 649-656 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Goto,Y.: "Pregnancy achieved by trausferring blastocysts into endomefrial sfroma in mice." Haman Reprod.7. 681-684 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nonogaki,T.: "Localization of Cu・Zn-superoxide diswutase in the human male genital organs." Human Reproduction. 7. 81-85 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] NODA,Y.: "Analysis of oviduct-derived embuyonic growth stiwule for activity." Int.J.Fertie.

    • Related Report
      1992 Annual Research Report
  • [Publications] NODA,Y.: "Biology of Germ Lines-in Animals and Man-in press" Japan Scientific Societies Press,

    • Related Report
      1992 Annual Research Report
  • [Publications] NODA,Y.: "Human Regroduction:Current Status/Future Prospect" Excerpta Medica,Amsterdam.,

    • Related Report
      1992 Annual Research Report

URL: 

Published: 1992-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi