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Fibroblast growth factor in saliva and oral lesions

Research Project

Project/Area Number 04557077
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionNiigata University School of Dentistry

Principal Investigator

SAKU Takashi  Niigata Univ.School of Dentistry, Professor, 歯学部, 教授 (40145264)

Co-Investigator(Kenkyū-buntansha) CHENG Jun  Niigata Univ.School of Dentistry, Assistant Professor, 歯学部, 助手 (40207460)
SUZUKI Makoto  Niigata Univ.School of Dentistry, Lecturer, 歯学部, 講師 (50107778)
SASAKI Hajime  Meiji Cell Technology Center, Head Investigator, 細胞工学センター, 主任
NAKAJIMA Tamio  Niigata Univ.School of Dentistry, Professor, 歯学部, 教授 (10014010)
HARA Kohji  Niigata Univ.School of Dentistry, Professor, 歯学部, 教授 (20018419)
辰巳 政弘  日本鋼管株式会社技術開発本部, 主任
人見 緑  新潟大学, 歯学部, 助手 (50218731)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥8,800,000 (Direct Cost: ¥8,800,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1992: ¥5,700,000 (Direct Cost: ¥5,700,000)
Keywordsfibroblast growth factor (FGF) / acidic FGF / basis FGF / immunochemistry / saliva / salivary gland / oral wound healing / epulides / 線維芽細胞成長因子 / 唾液線 / ELISA / in-site ハイブリダイゼーション / ウェスタンブロット / 歯周疾患 / マイクロファージ / in-situハイブリダイゼーション / マクロファージ / ドットブロット
Research Abstract

We have carried out the following experiments and determined the immunolocalization acidic/basic fibroblast growth factors (aFGF/bFGF) in various pathophysiologic conditions and the salivary FGF levels.
Salivary FGF level ; Using ELISA method, we determined bFGF in the human saliva and the bovine submandibular gland tissue. We could realize the detection sensitivity of 0.1 ng/ml of bFGF by the sandwith ELISA using two kinds of monoclonal antibodies. bFGF was enriched in salivary gland tissues at concentration of 1.1 ng/ml, that was 5 to 6 times of the normal human serum level. However, bFGF was not detected in the saliva with this system.
In order to determine the role of bFGF in gingival wound healing, we examined surgical materials of epulides containing various stages of granulation tissue, using the immunoperoxidase and immunofluorescence and formalin fixed paraffin sections. bFGF and heparan sulfate proteoglycan were co-immunolocalized in newly synthesized stroma in the middle organizing stage of granulation tissue. bFGF was also immunolocalized in macrophages, mast cells and vascular endothelial cells. bFGF-positive macrophages mostly increased in number at the middle stage. These findings suggest that bFGF is released from some of macrophages and mast cells into the extracellular milieu when granulation tissue is active in neovascularization but not yet for fibrosis.
Application of FGF determination to the clinical examination has not yet developed. However, we need more fundamental preparation before it is really applied to the saliva test.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] 朔,敬 他: "乳腺線維腺腫におけるヘパラン硫酸プロテオグリカンと線維芽細胞成長因子の免疫組織化学的局在" 日本病理学会会誌. 80. 155 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 程,〓 他: "唾液腺腺様嚢胞癌における線維芽細胞増殖因子の免疫組織化学的局在" 歯科基礎医学会雑誌. 33. 254 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 朔,敬 他: "塩基性線維芽細胞成長因子結合物質の免疫組織化学と口腔粘膜病変への応用" 歯科基礎医学会雑誌. 81. 114 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 人見,緑 他: "ヒト唾液腺の腫瘍における酸性および塩基性線維芽細胞増殖因子の免疫組織化学的局在" 歯科基礎医学会雑誌. 81. 283 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohtani,H.,et al.: "Immunocytochemical Localization of Basic Fibroblast Growth Factor in Carcinomas and Inflammetory Lesions of the Human Digestive Tract." Laboratory Investigation.68. 520-527 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Murata,M.,et al.: "Immunohistochemical localization of bFGF in gingival wound healing." Journal of Dental Research.74. 508 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Saku T,Rintoku S,Tsuda A,Sasaki H,Koiwai k.: "Immunohistochemical localization of heparan suafate proteoglycan and fibroblast growth factor in the fibroadenoma of mammary gland" Tr Soc Pathol Jpn.80(1). 155 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Cheng J,Okabe H,Sasaki H,Koiwai K,Saku T.: "Immunohistochemical localization of fibroblast growth factor in the adenoid cystic carcinoma of salivary gland" Jpn J Oral Biol.33(s). 254 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Saku T,Sasaki H.: "Immunohistochemistry of basic fibroblast growth factor binding and its application for oral mucosal legion" Tr Soc Pathol Jpn.81(1). 114 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiromi M,Suzuki M,Sasaki H,Saku T.: "Immunohistochemical localization of acidic and basic and basic fibroblast growth factor in the human salivary gland tumors" Tr Soc Pathol Jpn.81(1). 283 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohtani H,Nakamura S,Watanabe Y,Mizoi T,Saku T,Nagura H.: "Immunocytochemical localization of basic fibroblast growth factor in carcinomas and inflammatory lesions of the human digestive tract" Lab Invest.68(5). 520-527 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Murata M,Hara K,Yamamoto K,Saku T.: "Immunohistochemical localization of basic fibroblast growth factor in gingival granulation tissue" Jpn J Oral Biol.36(S). 195 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Murata M,Hara K,Saku T.: "Immunohistochemical localization of bFGF in gingival wound healing" J Dent Res.74(S). 508 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Murata M,Hara K,Saku T.: "Immunohistochemical localization of basic fibroblast growth factor in gingival granulation tissue" Niigata Dent J.25(2). 227 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohtani,H et al.: "Immunocytochemical Localization of Basic Fibroblast Growth Factor in Carcinoma and Inflammetory Lesions of the Human Digestive Tract." Lab Invest. 68. 520-527 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 朔 敬,佐々木 一: "塩基性線維芽細胞成長因子結合物質の免疫組織化学と口腔粘膜病変への応用" 日本口腔病理学会雑誌. 81. 114 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 人見 緑,鈴木 誠,佐々木 一,朔 敬: "ヒト唾液腺の腫瘍における酸性および塩基性線維芽細胞成長因子の免疫組織化学的局在" 日本口腔病理学会雑誌. 81. 283 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2017-10-06  

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