• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

BIOLOGICAL RECONSTRUCTION OF PERIODONTAL TISSUE USING CYTOKINE AND FIBROUS COLLAGEN MEMBRANE

Research Project

Project/Area Number 04557078
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

KUBOKI Yoshinori  HOKKAIDO UNIV., SCH.DENT., PROFESSOR, 歯学部, 教授 (00014001)

Co-Investigator(Kenkyū-buntansha) TAKITA Hiroko  HOKKAIDO UNIV., SCH.DENT., ASSISTANT, 歯学部, 教務職員 (30125330)
FUJISAWA Ryuichi  HOKKAIDO UNIV., SCH.DENT., LECTURER, 歯学部, 助手 (40190029)
MIZUNO Morimichi  HOKKAIDO UNIV., SCH.DENT., LECTURER, 歯学部, 助手 (10125354)
KAWAMAMI Masamitsu  HOKKAIDO UNIV., SCH.DENT., ASSOC.PROFESSOR, 歯学部, 助教授 (10133761)
KATO Hiroshi  HOKKAIDO UNIV., SCH.DENT., PROFESSOR, 歯学部, 教授 (60001020)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥10,600,000 (Direct Cost: ¥10,600,000)
Fiscal Year 1993: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥8,800,000 (Direct Cost: ¥8,800,000)
KeywordsBone morphogenetic protein / BMP / Carriers / Fibrous collagen membrane / Periodontal tissues / 骨形成タンパク質 / コラーゲン複合線維膜 / 人工歯根膜 / 不溶性骨基質 / ハイドロキシアパタイト / 生物学的再建
Research Abstract

Bone morphogenetic protein (BMP) is a growth and differntiation factor that stimulates immature mesemchymal cells to create a process similar to endochonral ossification when it is implanted with insoluble bone matrix (IBM) as a carrier. Roles of this carrier is still unkown and carriers other than IBM have not been investigated. We have deviced a fibrous collagen membrane (FCM), in order to combine it with BMP and to implant subcutanuously for ectopic bone formation. In this research project, first we succeeded in generating the ectopic formation of bone and cartilage independently in FCM/BMP implant. Thus, in order to clarify the mechanism behind the phenomenon of carrier-dependent pehonotype expression by BMP, we prepared and deviced at least six different types of carriers, combined them with the chromatographically-purified BMP, and compared the phenotype expression upon implantation. New BMP-carriers other than IBM and FCM included the porous particles of hydroxyapatite (PPHAP), solid particles of hydroxyapatite (SPHAP), honeycomb shaped hydroxyapatite and fibrous glass membrane (fine and coarse FGM). Most striking finding was that the coarse FGM/BMP induced only cartilage inside the membrane, while the PPHAP/BMP induced only bone within the pore of the PPHAP.It was found that SPHAP/BMP and the fine FGM/BMP did not induce bone nor cartilage at all. Honeycomb HAP, which is consistedof cylindrical blocks (1.4 x 0.7 mm) with seven tubular holes of 0.12 mmdiameter, induced very unique shape of bone, generating the columnar bone in the center of each tubular hole, not on the inner surface of tubular holes. It was interpreted that pores in the PPHAP provided the space for vasculature which was feasible for direct bone formation, while FGM rejected vasculature but permitted mesenchymal cells entering into the membrane, which provided a feasible circumstances for cartilage formation. Above results indicate that geometrical factors are important in the use of FCM and

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Kuboki,Y.: "Amino-Terminal Location of Pyridinoline in Dentin Collagen" Connective Tissue Research. 29. 87-98 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujisawa,R.: "Differences in composition of cell-attachment sialoproteins between dentin and bone" J.Dent.Res.72. 1222-1226 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sasano,Y.: "BMPs induced direct bone formation in ectopic sites independent of the endochondral ossification in vivo" The Anatomical Record. 236. 373-380 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kuboki,Y.: "Time-Dependent Changes of Collagen Cross-Links and their Precursors in the Culture of Osteogenic Cells" Calcif Tissue Int. 50. 473-480 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mizuno,M.: "An Osteonectin-Like in the Matrix of Cultured Osteogenic Cell-Line MC3T3-E1,which is Associated with Calcification" Calcif Tissue Int. 51. 156-161 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ono,I.: "A Study on Bone Induction in Hydroxyapatite Combined with Bone Morphogenetic Protein" Plastic and Reconstructive Surgery. 90. 870-879 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshinori Kuboki: "Apatite" Japanese Association of Apatite Science, 536 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kuboki, Y.: "Amino-Terminal Location of Pyridinoline in Dentin Collagen" Connect.Tissue Res.29. 87-98 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujisawa, R.: "Differences in composition of cell-attachment sialoproteins between dentin and bone." J.Dent.R.72. 1222-1226 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sasano, Y.: "BMPs induced direct bone formation in ectopic sites independent of the endochondral ossification in vivo." The Anatomical Record. 236. 373-380 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kuboki, Y.: "Time-Dependent Changes of Collagen Cross-Links and their Precursors in the Culture of Osteogenic Cells." Calcif Tissue Int. 50. 473-480 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mizuno, M.: "An Osteonectin-Like in the Matrix of Cultured Osteogenic Cell-Line MC3T3-E1, which is Associated with Calcification." Calcif Tissue Int. 51. 156-161 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ono, I.: "A Study on Bone Induction in Hydroxyapatite Combined with Bone Morphogenetic Protein." Plastic and Reconstructive Surgery. 90. 870-879 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kuboki,Y.: "Amino-Terminal Location of Pyridinoline in Dentin Collagen" Connective Tissue Research. 29. 87-98 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Fujisawa,R.: "Differences in composition of cell-attachment sialoproteins between dentin and bone" J.Dent.Res.72. 1222-1226 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sasano,Y.: "BMPs induced direct bone formation in ectopic sites independent of the endochondral ossification in vivo" The Anatomical Record. 236. 373-380 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kuboki,Y.: "Time-Dependent Changes of Collagen Cross-Links and their Precursors in the Culture of Osteogenic Cells" Calcif Tissue Int. 50. 473-480 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Mizuno,M.: "An Osteonectin-Like in the Matrix of Cultured Osteogenic Cell-Line MC3T3-E1,which is Associated with Calcification" Calcif Tissue Int. 51. 156-161 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ono,I.: "A Study on Bone Induction in Hydroxyapatite Combined with Bone Morphogenetic Protein" Plastic and Reconstructive Surgery. 90. 870-879 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yoshinori Kuboki: "Apatite" Japanese Association of Apatite Science, 536 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yoshinori Kuboki,Masumi Okuguchi et al.: "Amino-Terminal Location of Pyridinoline in Dentin Collagen" Connective Tissue Research. 29. 87-98 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yasuyuki Sasano,Yoshinori Kuboki et al.: "BMPs induce direct bone formation in ectopic sites independent of the endochondral ossification in vivo" Anatomical Record in press. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Morimichi Mizuno,Yoshinori Kuboki et al.: "An Osteonectin-Like Protein in the Matrix of Cultured Osteogenic Cell-Line MC3T3-E1,Which is Associated with Calcification" Calcif Tissue Int. 51. 156-161 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] R.Fujisawa,Y.Kuboki: "Affinity of Bone Sialoprotein and Several Other BOne and Dentin Acidic Proteins to Collagen Fibrils" Calcif Tissue Int. 51. 438-442 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ichiro Ono,Yoshinori Kuboki et al.: "A Study on Bone Induction in Hydroxyapatite Combined with Bone Morphogenetic Protein" Plastic and Reconstructive Surgery. 90. 870-879 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yoshinori Kuboki,Akira Kudo et al.: "Time-Dependent Changes of Collagen Cross-Links and their Precursors in the Culture of Osteogenic Cells" Calcif Tissue Int. 50. 473-480 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yoshinori Kuboki,Hiroko Takita,Mariko Tazaki,Morimichi Mizuno et al.: "Apatite" Japanese Association of Apatite Science, 536 (1992)

    • Related Report
      1992 Annual Research Report

URL: 

Published: 1992-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi