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Studies on the Moleculur and Biological Features of Oral Cancer Cells for the Developments of the Gene Diagnosis

Research Project

Project/Area Number 04557090
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 外科・放射線系歯学
Research InstitutionThe University of TOKUSHIMA

Principal Investigator

MAGAYAMA Masaru  The University of TOKUSHIMA,School of Dentistry, Professor, 歯学部, 教授 (30022867)

Co-Investigator(Kenkyū-buntansha) MORISHITA Hideaki  Mochida Phamaceutical Company, Bio-science Institute, バイオサイエンス研究所, 主幹
HAYASHI Eiji  The University of TOKUSHIMA,School of Dentistry, Research Associates, 歯学部, 助手 (50173000)
RIKIMARU Koichi  The University of TOKUSHIMA,School of Dentistry, Associate Professor, 歯学部, 助教授 (40220800)
森下 英明  持田製薬(株), バイオサイエンス研究所, 主幹
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥16,800,000 (Direct Cost: ¥16,800,000)
Fiscal Year 1994: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1993: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1992: ¥9,900,000 (Direct Cost: ¥9,900,000)
KeywordsOral Cnacer / Squamous Cell Carcinoma / EGF Receptor / p53 / Cadherin / Monoclonal Antidody / 遺伝子診断 / 分子細胞生物学
Research Abstract

In order that the gene diagnosis technique may be introduced into the field of oral cancer, we attempted to find out the biological features of squamous cell carcinoma, which is the majority of oral cancers.
1)The frequency of mutations in p53 tumor-suppressor gene was studied by the method of polymerase chain reaction followed by single-strand conformation polymorphism (PCR-SSCP) . The assay revealed that overall 63% of oral squamous cell carcinomas carried the mutated p53 gene within the regions of exon 5 to 8, suggesting that the mutation of p53 gene is the most frequent gene alteration occurred in oral squamous cell carcinoma. In order to clear the relationship between the gene mutation and the detectability of its protein in immuno-histochemistry, we injected the tumor cells, of which the patterns of p53 gene mutation were well characterized, into SCID mice and made tumors on animals, and then cross sections were prepared and stained with anti-p53 protein antibodies. The detection … More of p53 protein in the cross sections was correlated with the type of gene mutations, mis-sense mutation, suggesting the limitation of immuno-histochemistry to detect the p53 gene mutations.
2)We investigated the gene amplification of erbB-1 and its expression. Out of four cell lines, which were newly established from the oral squamous cell carcinoma patients, only one carried the amplified gene, as was consistent with our previous observation. Interestingly we noticed that some cell lines expressed unexpectedly large number of EGF receptor on the surface. The kinetic analysis of EGF receptor revealed that these cells showed delayd pattern of the protein degradation.
3)We investigated the effects of EGF administration on the formation of lung metastases of EGF receptor-hyperproducing squamous cell carcinoma cells in SCID mice. The results suggested that EGF administered somatically did not exert the obvious effects on the formation of metastases.
4)We studied the patterns of the protein secretion from the normal epithelial cells and squamous cell carcinoma cells, and compared the differences of them using a hybridoma technique. As results, two monoclonal antibodies were obtained, one of which recognized the protein that secreted by cancer cells rather than normal cells and another one recognized the protein decreased in secretion in caner cells. These findings suggest the possibility to know the novel characteristics of oral squamous cell carcinoma after the molecular cloning of these proteins was accomplished successfullyaccomplished. Less

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] 倉郷貴敏: "口腔扁平上皮癌細胞におけるEGFレセプターの過剰発現機構" 四国歯学会雑誌. 1. 49-62 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 布袋屋智朗: "口腔扁平上皮癌細胞におけるE-カドヘリンの発現に関する研究" 四国歯学会雑誌. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 中野孝三郎: "無蛋白培養ヒト口腔扁平上皮癌細胞の分泌物に対するモノクローナル抗体の作製とその特性" 四国歯学会雑誌. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 林英司: "4種のヒト口腔癌細胞株の樹立とその性状" 日本口腔科学会雑誌. 44. 47-54 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Akira Ishisaki: "Identification and characterization of autocrine-motility-factor-like activity in oral squamous-cell -carcinoma cells" International Journal of Cancer. 59. 783-788 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 力丸浩一: "SCIDマウスを用いた扁平上皮癌のin vivo転移モデルにおけるヒトEGFの影響" (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takatoshi KURASATO: "Mechanism of Hyperproduction of Epidermal Grouth Factor Receptors on Squamous Cell Carcinoma Cells" Shikoku Dental Reserch.7 (1). 49-62 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toshiaki HOTEIYA: "Study of E-Cadherin Expression in Oral Squamouse Cell Carcinoma Cell Line" Shikoku Dental Reserch.(now printing).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kohzaburo NAKANO: "Production and Characterization of Monoclonal Antibodies Raised against the Supernatants from Human Oral Squamouse Cell Carcinoma Cells Cultured in Protein-Free Medium" Shikoku Dental Reserch.(now printing).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Eiji HAYASHI: "Establishment and Characterization of Four Human Oral Cancer Cell Lines" J.Jpn.Stomatol.Soc.44 (1). 47-54 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Akira Ishisaki: "Identification and characterization of autocrine-motility-factor-like activity in oral squamouse-cell-carcinoma cells" Int.J.Cancer. 59. 783-788 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] koichi RIKIMARU: "Effects of Human EGF on Pulmonary Metastasis of Human Squamouse Cell Carcinomas in SCID Mice" J.Jpn.Stomatol.Soc.44(2) (now printing).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 倉郷貫敏: "口腔扁平上皮癌細胞におけるEGFレセプターの過剰発現機構" 四国歯学会雑誌. 1. 49-62 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 布袋屋智明: "口腔扁平上皮癌細胞におけるE・カドヘリンの発現に関する研究" 四国歯学会雑誌. (印刷中).

    • Related Report
      1994 Annual Research Report
  • [Publications] 中野孝三郎: "無蛋白培養ヒト口腔扁平上皮癌細胞の分泌物に対するモノクローナル抗体の作製とその特性" 四国歯学会雑誌. (印刷中).

    • Related Report
      1994 Annual Research Report
  • [Publications] 林 英司: "4種のヒト口腔癌細胞株の樹立とその性状" 日本口腔科学会雑誌. 44. 47-54 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Akira Ishisaki: "Identification and characterization of autocrine-motility-factor-like activity in oral squamous-cell-carcinoma cells" International of Cancer. 59. 783-788 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 力丸浩一: "SCIDマウスを用いた扁平上皮癌のin vivo転移モデルにおけるヒトEGFの影響" 日本口腔科学会雑誌. (印刷中).

    • Related Report
      1994 Annual Research Report
  • [Publications] Eiki SAKAI: "THE p53 TUMOR-SUPPRESSOR GENE AND ras ONCOGENE MUTATIONS IN ORAL SQUAMOUS-CELL CARCINOMA" International Journal of Cancer. 52. 867-872 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 倉郷貴敏: "口腔扁平上皮癌細胞におけるEGFレセプターの過剰発現機構" 四国歯学会雑誌. 第7巻. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Eiki Sakai: "The p53 tumor-suppressor gene and ras oncogene mutations in oral squamous-cell carcinoma." International Journal of Cancer. 52. 867-872 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 力丸 浩一: "腫瘍とEGF" 臨床消化器内科. 11. 1855-1862 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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