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Development of immunological method for prevention of dental caries using genetic engineering

Research Project

Project/Area Number 04557095
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 小児・社会系歯学
Research InstitutionKYUSHU UNIVERSITY (1993-1994)
国立予防衛生研究所 (1992)

Principal Investigator

KOGA Toshihiko  Kyushu University, Faculty of Dentistry, Professor, 歯学部, 教授 (10037541)

Co-Investigator(Kenkyū-buntansha) NAKANO Yoshio  Kyushu University, Faculty of Dentistry, Instructor, 歯学部, 助手 (80253459)
OHO Takahiko  Kyushu University, Faculty of Dentistry, Assistant Professor, 歯学部, 講師 (50160940)
中井 素行  ライオン(株)オーラルケア研究所, 研究員
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥10,500,000 (Direct Cost: ¥10,500,000)
Fiscal Year 1994: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1993: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1992: ¥4,800,000 (Direct Cost: ¥4,800,000)
KeywordsDental caries / Streptococcus mutans / Adherence factor / Protein antigen / Glucosyltransferase / Fusion protein / スクロース / 付着 / 免疫 / 遺伝子
Research Abstract

The first step for induction of dental caries by Streptococcus mutans is the adherence of the organism to tooth surfaces. Surface protein antigen (PAc) is considered to participatein the sucrose-independent attachment of the cell to acquired pellicles on tooth surfaces. On the other hand, glucosyltransferase (GTF) is important in the sucrose-dependent adherence of S.mutans.Our epitope mapping using many decapeptides showed that antigenic epitopes were located in the N-terminal A-repeat and middle region of PAc.Moreover, the A-repeat bound to salivary components. We have, therefore, constructed a fusion protein of the fragment corresponding to the A-repeat of PAc and the fragment corresponding to the sucrose-binding site of GTF-I,using an expression plasmid vector pTrc99A.Escherichia coli XL1-blue containing the chimeric plasmid pFPG22 expressed a fusion protein of PAc and GTF-I that reacted with both rabbit anti-PAc serum and antiGTF-I serum. Subcutaneous immunization with this fusion protein induced high titers of antibodies to both adherence factors, and these antibodies inhibited GTF-I, suggesting that the fusion protein may be useful for immunological prevention of dental caries.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] N. Okahashi 他5名: "Identification of antigenic epitopes in an alanine-rich repeating region of a surface antigen of Streptococcus mutans" Infection and Immunity. 61. 1301-1306 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M. Nakai 他3名: "Saliva-binding region of Streptococcus mutans surface protein antigen" Infection and Immunity. 61. 4344-4349 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M. Higuchi 他5名: "Identification of two distinct NADH oxidase corresponding to H2O2-forming oxidase and H2O-forming oxidase induced in Streptococcus mutans" Journal of General Microbiology. 139. 2343-2351 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K. Matsushita 他4名: "Identification of the antigenic epitopes in a surface protein antigen of Streptococcus mutans in humans" Infection and Immunity. 62. 4034-4042 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] G. Hajishengallis 他2名: "Affinity and specificity of the interactions between Streptococcus mutans antigen I/II and salivary components" Journal of Dental Research. 73. 1493-1502 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] N. Okahashi 他1名: "Pulse-field gel electrophoresis: large scale restriction maps" Methods in Gene Technology. 2. 207-226 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 古賀敏比古: "ワクチンハンドブック" 丸善株式会社, 311 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T. Koga: "Streptococcal Genetics-1994" International Association of Biological Standardization (印刷中), (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] N.Okahashi et al.: "Identification of antigenic epitopes in an alanine-rich repeating region of a surface antigen of Streptococcus mutans" Infection and Immunity. vol. 61. 1301-1306 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Nakai et al.: "Saliva-binding region of Streptococcus mutans surface protein antigen" Infection and Immunity. vol. 61. 4344-4349 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Higuchi et al.: "Identification of two distinct NADH oxidase corresponding to H2O2-forming oxidase and H2O-forming oxidase induced in Streptococcus mutans" Journal of General Microbiology. vol.139. 2343-2351 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Matsushita et al.: "Identification of the antigenic epitopes in a surface protein antigen of Streptococcus mutans in humans" Infection and Immunity. vol. 62. 4034-4042 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] G.Hajishengallis et al.: "Affinity and specificity of the interactions between Streptococcus mutans antigen I/II and salivary components" Journal of Dental Research. vol.73. 1493-1502 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] N.Okahashi et al.: "Pulse-field gel electrophoresis : large scale restriction maps" Methods in Gene Technology. vol.2. 207-226 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K Matsushita: "Identificaion of the antigenic epitopes in a surface protsin oritigen of Streptococcus Mutans in humans" Infection and Immunity. 62. 4034-4042 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] G.Hajishengallis: "Affinity and specificity of the interactions between Streptococcus mutans antigen I/II and salivary components" Journal of Dental Research. 73. 1493-1502 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.Okahashi: "Pulse-field gel electrophoresis:large scale restriction maps" Methods in Gene Technology. 2. 207-226 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 古賀敏比古: "「平成5年歯科疾患実態調査の概要」を分析する" 歯界展望. 84. 661-670 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 古賀敏比古: "ワクチンハンドブック" 丸善株式会社, 311 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Koga: "Streptococcal Genetics-1994" International Association of Biological Standardization(印刷中), (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.Okahashi: "Identification of antigenic epitopes in an alanine-rich repeating region of a surface antigen of Streptococcus mutans." Infection and Immunity. 61. 1301-1306 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Nakai: "Saliva-binding region of Streptococcus mutans surface protein antigen." Infection and Immunity. 61. 4344-4349 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Higuchi: "Identification of two distinct NADH oxidases corresponding to H_2O_2-forming oxidase and H_2O-forming oxidase induced in Streptococcus mutans." Journal of General Microbiology. 139. 2343-2351 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 古賀敏比古: "齲蝕細菌の分子遺伝学とその応用" 福岡医学雑誌. 84. 493-496 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takahashi,I.他7名: "Genetic control of immune responses to synthetic peptides of a Streptococcus mutans surface protein antigen in mice" Infect.Immun.60. 623-629 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ohahashi,N.他5名: "Identification of antigenic epitopes in an alanine-rich repeating region of a surface antigen of Streptococcus mutans" Infect.Immun.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 高橋 一郎、他1名: "Streptococcus mutansの菌体表層タンパク質抗原に由来する合成ペプチドはStreptococcus mutansの口腔への定着を抑制する" 臨床免疫. 24. 124-132 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 高橋 一郎、他1名: "Epitope scanningーマルチピンテクノジーを利用した抗原エピトープの解析" 生体防御. 9. 107-112 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 岡橋 暢夫、他1名: "合成ペプチドを用いたエピトープの同定法" 実験医学. 10. 85-89 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Okahashi,N.他1名: "Methods in Gene Technology,Vol.2" Dale,J.W.and Sanders,P.G.JAI Press, (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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