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Analyzes of dynamic molecular organization of the membrane skeleton

Research Project

Project/Area Number 04557116
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 医学一般
Research InstitutionOsaka University

Principal Investigator

SOBUE Kenji  Osaka Univ.Med.Sch.Prof., 医学部, 教授 (20112047)

Co-Investigator(Kenkyū-buntansha) HAYASHI Kenichiro  Osaka Univ.Med.Sch.Assist.Prof., 医学部, 助手 (90238105)
TANAKA Junya  Osaka Univ.Med.Sch.Assist.Prof., 医学部, 助手 (70217040)
INUI Makoto  Osaka Univ.Med.Sch.Assist.Prof., 医学部, 助手 (70223237)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥17,600,000 (Direct Cost: ¥17,600,000)
Fiscal Year 1993: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1992: ¥15,100,000 (Direct Cost: ¥15,100,000)
KeywordsActin / Caldesmon / Myosin / Annexin VI / Cytoskeleton / Calspectin / 間接螢光抗体法 / トロポミオシン / 細胞内局在
Research Abstract

1. Cell/substratum adhesive structures of Rous sarcoma virus transformed cells : We observed that Rous sarcoma virus transformed-cells developed both podosomes under the center area of ventral plasma membrane and peripheral adhesion along the cell periphery. Podosomes moved actively on a time scale of minutes, while peripheral adhesions scarecely moved. Contractlie proteins (actin, myosin, caldesmon and tropomyosin) were enriched within the podosomes, which may be responsible for the active movement of podosomes. By contrast, the proteins were not acumulated in peripheral adhesions, where integrins, alternatively, were enriched. These findings suggest that the two adhesive structures may participate in different functions when the cells invade into tissues ; firstly, the cells attach stably on the tissue via peripheral adhesions, and secondly, the podosomes invade into the tissue while activly moving and secreting proteases.
2. Target proteins of annexin VI in brain : To elucidate the r … More oles of annexin VI, we searched proteins interacting with annexin VI in the rat brains. Annexin VI bound to 15 spieces of proteins in a Ca^<2+> and phospholipid dependent manner. Of these proteins, about 5 proteins were enriched in the cytoskeletal fraction. One of these proteins was identified to be calspectin. Annexin VI bound to calspectin, inhibiting the F-actin crosslinking activity of calspectin. These observations sugget that annexin VI may be involved in many neuronal functions including Ca^<2+>-dependent regulation of the membrane cytoskeleton.
Actin and actin binding proteins, and motile cell/substratum adhesive structures of neural cells : Cultured neurons possess highly motile cell/substratum adhesive structures called growth cones at tips of neurites. Actin filaments, myosin, caldesmon, tropomyosin and gelsolin were enriched within the growth cones, which may be responsible for the active movement and Ca^<2+> sensitivity of growth cones. We found out that cultured astrocytes also developed highly motile adhesive structures along the processes and termed the adhesion ruffling contact. The movement of ruffling contact was similar to that of growth one. The contractile proteins and gelsolin were also accumulated in the adhesions of astrocytes as well as in growth cones and the podosomes. These findings show that the contratile protein and gelsolin may be molecular bases for the active movement of these motile adhesion. Less

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Hayashi,K.: "Genomic structure of the human caldesmon gene." Proc.Natl.Acad.Sci.USA. 8. 1212-1212 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kitagawa: "The synapsin I brain distribution in ischemia." Neuroscience. 46. 287-299 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kitagawa: "The characteristics of blood-brain barrier in three different conditions-infarction,selective neuronal death and selective loss of presynaptic terminals-following cerebral ischemia." Acta Neuropathol.84. 37-386 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sobue,K.: "Molecular approach for smooth muscle differentiation:association of caldesmon isoformal interconversion with phenotypic modulation of smooth muscle cells." Biomed.Res.14. 147-153 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sobue,K.: "Actin-based cytoskeleton in growth cone activity." Neurosci.Res.18. 91-102 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tanaka,J.: "Morphological and biochemical analyses of contractile proteins(actin,myosin,caldesmon and tropomyosin)in normal and transformed cells." J.Cell Sci.104. 595-606 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tanaka,J.: "Gelsolin is localized in neuronal growth" Dev.Brain. 76. 26-27 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Haruna,M.: "Common structural and expressional properties of vertebrate caldesmon genes." Biochem.Biophys.Res.Commun.197. 145-153 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nakamura,N.: "Rous sercoma virus-transformed cells develop peculiar adhesive structures along the cell periphery." J.Cell Sci.106. 1057-1069 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tanaka,J.: "Localization and characterization of gelsolin in nervous tissues--gelsolin is specifically enriched in myelin-forming cells--" J.Neurosci.(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sobue,K.: "Ca^<2+>-dependent regulation of the neuronal cytoskeleton.in Neuronal Cytoskeleton" CRC Press, 335 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inui,M.: "Annexin VI-binding proteins in brain.in Neuronal Cytoskeleton" CRC Press, 335 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tanaka,J.: "Gelsolin is involved in myelin formation.in Neuronal Cytoskeleton" CRC Press, 335 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ozawa,K.: "Compartmentation of p60^<c-src> complex in developing rat forebrain membrane.in Neuronal Cytoskeleton" CRC Press, 335 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hayashi, K.: "Genomic structure of the human caldesmon gene." Pro.Natl.Acad.Sci.USA. 89. 12122-12126 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kitagawa, K.: "The synapsin I brain distribution in ischemia." Neuroscience. 46. 287-299 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kitagawa, K.: "The characteristics of blood-brain barrier in three different conditions-infarction, selective neuronal death and selective loss of presynaptic terminals-following cerebral ischemia." Acta Neuropathol. 84. 378-386 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tanaka, J.: "Morphological and biochemical analyzes of contractile proteins (actin, myosin, caldesmon and tropomyosin) in normal and transformed cells." J.Cell Sci.104. 595-606 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tanaka, J.: "Gelsolin is localized in neuronal growth cones." Dev.Brain Res.76. 268-271 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sobue, K.: "Molecular approach for smooth muscle differentiation : association of caldesmon isoformal interconversion with phenotypic modulation of smooth muscle cells." Biomedical Res.suppl.14. 147-153 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sobue, K.: "Actin-based cytoskeleton in growth cone activity." Neurosci.Res.18. 91-102 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Haruna, M.: "Common structural and expressional properties of vertebrate caldesmon genes." Biochemi.Biophys.Res.Commun.197. 145-153 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nakamura N.: "Rous sercoma virus-transformed cells develop peculiar adhesive structures along the cell periphery." J.Cell Sci.106. 1057-1069 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tanaka, J.: "Localization and characterization of gelsolin in nervous tissues-gelsolin is specifically enriched in myelinforming cells-" J.Neurosci.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sobue, K.: Ca^<2+>-dependent regulation of the neuronal cytoskeleton.Neuronal Cytoskeleton (Hirokawa N.ed.) CRC Press., 259-274 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inui, M.: Annexin VI-binding proteins in brain.Neuronal Cytoskeleton (Hirokawa N.ed.) CRC Press., 275-284 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tanaka, J.: Gelsolin is involved in myelin formation.Neuronal Cytoskeleton (Hirokawa N.ed.) CRC Press., 145-158 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ozawa, K.: Compartmentation of p60^<c-src> complex in developing rat forebrain membrane.Neuronal Cytoskeleton (Hirokawa N.ed.) CRC Press., 305-316 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sobue,K.: "Molecular approach for smooth muscle differentiation:association of caldesmon isoformal interconversion with phenotypic modulation of smooth muscle cells." Biomed.Res.14. 147-153 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sobue,K.: "Actin-based cytoskeleton in growth cone activity." Neurosci.Res.18. 91-102 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tanaka,J.: "Morphological and biochemical analyses of contractile proteins(actin,myosin,caldesmon and tropomyosin)in normal and transformed cells." J.Cell Sci.104. 595-606 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Haruna,M.: "Common structural and expressional properties of vertebrate caldesmon" Biochem.Biophys.Res.Commun.197. 145-153 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nakamura,N.: "Rous sercoma virus-transformed cells develop peculiar adhesive structures along the cell periphery." J.Cell Sci.106. 1057-1069 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tanaka,J.: "Localization and characterization of gelsolin in nervous tissues--gelsolin is specifically enriched in myelin-forming cells--" J.Neurosci.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sobue,K.: "Ca^<2+>-dependent regulation of the neuronal cytoskeleton.in Neuronal Cytoskeleton" CRC Press, 335 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Inui,M.: "Annexin VI-binding proteins in brain.in Neuronal Cytoskeleton" CRC Press, 335 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kitagawa,K.: "The synapsin I brain distribution in ischemia." Neuroscience. 46. 287-299 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hayashi,K.: "Genomic structure of the human caldesmon gene." Proc.Natl.Acad.Sci.USA. 89. 12122-12126 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tanaka,J.: "Morphological and biochemical analyses of contractile proteins(actin,myosin,caldesmon and tropomyosin)in normal and transformed cell." J.Cell Sci.(1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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