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Development of an imaging system for genome mapping

Research Project

Project/Area Number 04557119
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Human genetics
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

YOSHIDA Michihiro  Hokkaido Univ. ; Fac.Sci. ; Prof., 理学部, 教授 (60001765)

Co-Investigator(Kenkyū-buntansha) YAMADA Michio  Flovel Inc.Software Investigator, 研究職員
UMEHARA Chizuko  Hokkaido Univ. ; Fac.Sci. ; Research Assist., 理学部, 教務職員
MASUDA Ryuich  Hokkaido Univ., Fac.Sci. ; Assist.Prof., 理学部, 助手 (80192748)
ABE Shuichi  Hokkaido Univ., Fac.Sci. ; Assoc.Prof., 理学部, 助教授 (80125278)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥15,500,000 (Direct Cost: ¥15,500,000)
Fiscal Year 1993: ¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1992: ¥10,300,000 (Direct Cost: ¥10,300,000)
KeywordsGenome / Mapping / Image analyzer / FISH / Chromosomes / In situ hybridization / プローブ / 画像処理
Research Abstract

Development of an imaging system for chromosome analysis, particularly fluorescence in situ hybridization (FISH), is important for the human genome mapping. This research project aimed for development of an easy-and-cheap digital imaging processor for chromosome mapping :
(1) For capture FISH-metaphases as digital imaging we improved a CCD camera and software.
(2) Using the improved camera, we also developed and improved software for multicolor FISH-mapping.
Our improvements of digital imaging processors by this project have provided more rapid analysis of FISH-signals and chromosomes. However, it is necessary to do more improvement for image enhancements for clearer image and remove background distortions.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Yoshida,M.C.: "Recent advances in fluorescence in situ hybridization" J.Radiat.Res.Suppl.33. 54-60 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hirashima,M.等: "Nucleotide sequence of the third eytekine LD78 gene and mapping of all three LD78 gene loci to human chromosome 17" DNA Sequence. 3. 203-212 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsuoka,R.,等: "Human smooth muscle myosin neavy chain gene mapped to chromosomal region 16q12" Amer.J.Med.Genet.46. 61-67 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ishida,A.,等: "Cloning and chromosome mapping of the human Mel-18 gene which encodes a DNA-binding protein with a new“RING-finger"motif" Gene. 129. 249-255 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Asano,H.,等: "The mouse Mel-18“RING-finger"gene:genomic organization,promoter analysis and chromosomal assignment" DNA Sequence. 3. 369-377 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kagiyama,N.等: "A novel fluorescent method for in situ hybridization" Acta Histochem.Cytochem.26. 441-445 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 吉田廸弘: "(遺伝子の染色体へのマッピング技術)ゲノム解析研究" 共立出版(松原、榊編), 720 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 吉田廸弘: "核酸V(細胞工学的技術)「in situハイブリダイゼーション」" 東京化学同人(日本生化学学会編), 338 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshida MC: "Recent advances is fluorescence in situ hybridization." J.Radiat.Res.Suppl.33. 54-60 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sofuni T & Yoshida MC: "Combined use of several mitogens for miotic stimulation to human lymphocytes." J.Radiat.Res.Suppl.33. 222-230 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hirashima M, Ono T, Nakao M, Nishi H, Kimura A, Nomiyama H, Hamada F, Yoshidas MC & Shimada K: "Nucleotide sequence of the third cytokine LD78 gene and mapping of all three LD78 gene loci to human chromosome 17." DNA Sequence. 3. 203-212 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshida MC: "Technique for gene mapping." PNE. 38(3). 558-562 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ono T, Oshida T & Yoshida MC: "In situ hybridization with radioisotope." Tissue Cult.19(1). 3-7 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshida MC, Sugawara S & Nakano H: "Chromosome mapping by slide-PCR amplification." Tissue Cult.19(1). 18-21 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ishida A, Asano H, Hasegawa M, Koseki H, Ono T, Yoshida MC, Taniguchi M & Kanno M: "Cloning and chromosome mapping of the human Mel-18 gene which encodes a DNA-binding protein with a new RING-finger motif." Gene. 129. 249-255 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Asano H, Ishida A, Hasegawa M, Ono T, Yoshida MC, Taniguchi M & Kanno M: "The mouse Mel-18 RING-finger gene : genomic organization, promotor analysis and chromosomal assignment." DNA Sequence. 3. 369-377 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kagiyama N, Yoshida K, Hamabata T, Juni N, Awasaki T, Momiyama M, Kondoh Y, Yoshida MC & Hori SH: "A novel fluorescent method for in situ hybridization. Acta Histochem." Cytochem.26(5). 441-445 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshida MC: Chromosome mapping by in situ hybridization IN Genetic Engineering Handbook. Yodosha Press, Tokyo, 271-277 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshida,M.C.: "Recent advances in fluorescence in situ hybridization" J.Radiat.Res.Suppl.33. 54-60 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hirashima,M.等: "Nucleotide sequence of the third cytokine LD78 gene and mapping of all three LD78 gene loci to human chromosome 17" DNA Sequence. 3. 203-212 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Matsuoka,R.,等: "Human smooth muscle myosin heavy chain gene mapped to chromosomal region 16q12" Amer.J.Med.Genet.46. 61-67 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ishida,A.,等: "Cloning and chromosome mapping of the human Mel-18 gene which encodes a DNA-binding protein with a new"RING-finger"motif" Gene. 129. 249-255 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Asano,H.,等: "The mouse Mel-18"RING-finger"gene:genomic organization,promoter analysis and chromosomal assignment" DNA Sequence. 3. 369-377 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kagiyama,N.等: "A novel fluorescent method for in situ hybridization" Acta Histochem.Cytochem.26. 441-445 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 吉田廸弘: "(遺伝子の染色体へのマッピング技術)ゲノム解析研究" 共立出版(松原、榊編), 720 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 吉田廸弘: "核酸V(細胞工学的技術)「in situハイブリダイゼーション」" 東京化学同人(日本生化学学会編), 338 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yoshida,M.C.: "Recent advances in fluorescence in situ hybridization" J.Radiat.Res.Suppl.33. 54-60 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sofuni,T.&Yoshida,M.C.: "Combined use of several mitogens for mitotic stimulation to human lymphocytes" J.Radiat.Res.Suppl.33. 223-230 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hirashima,M.,---Yoshida,M.C.,---: "Nucleotide sequence of the third cytokine LD78 gene and mapping of all three LD78 gene loci to human chromosome17" DNA Sequence. 3. 203-212 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 吉田 廸弘: "スライド-PCR法による染色体マッピング" 組織培養. 19. 18-21 (193)

    • Related Report
      1992 Annual Research Report
  • [Publications] 吉田 廸弘: "遺伝子の染色体へのマッピング技術" 蛋白質核酸酵素. 38. 558-562 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 吉田 廸弘: "遺伝子マッピングのためのハイブリッドパネルの作製" 細胞工学ハンドブック. 107-110 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 吉田 廸弘: "医科分子生物学(村松正実編)" 南江堂, 479 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 吉田 廸弘: "臨床遺伝学III(古庄,外村,清水,北川編)" 診断と治療社, 643 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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