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Establishment of in vitro screenin models for new drugs against brain edema

Research Project

Project/Area Number 04557124
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用薬理学・医療系薬学
Research InstitutionOsaka University

Principal Investigator

BABA Akemichi  Osaka Univ., Fac.Pharmaceutical Sci., Professor, 薬学部, 教授 (70107100)

Co-Investigator(Kenkyū-buntansha) KOYAMA Yutaka  Osaka Univ., Fac.Pharmaceutical Sci., Associate Res., 薬学部, 助手 (00215435)
MATSUDA Toshio  Osaka Univ., Fac.Pharmaceutical Sci., Associate Prof., 薬学部, 助教授 (00107103)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1992: ¥3,700,000 (Direct Cost: ¥3,700,000)
Keywordsbrain edema / cultured cells / astrocytes / endothelial cells / glutamate / endothelin / 血液-脳関門 / 膨化 / タウリン
Research Abstract

1)In vitro model of cytotoxic edema : L-Glutamate-induced swelling of cultured astrocytes showed two components. Quisqualate induced the rapid component, while threo-hydroxy-aspartate did the delayd one. These results suggest that the rapid swelling was caused by activation of L-glutamate receptors and the delayd one was by that of transporters. Swollen astrocytes release taurine. Thus, as a functional change of astrocytes, effects of L-glutamate and its analogues on taurine release were examined. All of L-glutamate analogues to induce astrocytic swelling stimulated taurine release from cultured astrocytes. Kainate released taurine without swelling. These results indicate that these are two mechanisms in L-glutamate-induced astrocytic taurine release. This will be published in a coming volume of "J.Neurosci.Res.".
2)In vitro model for blood-brain barrier : We showed that endothelins modulate morphological differentiation of cultured astrocytes (Brain Res., 600). And accompanying with the morphological changes, astrocytic cytoskeletal actin is re-organized. These results suggest that endothelins are important factors to make blood-brain barrier tight. This will be published in a coming volume of "Neuroscience". Actin re-organization of endothelial cells underlies permeability of blood vessels. Endothelins had no effects on cytochalasin B-induced actin re-organization of endothelial cells from bovine brain.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Y.Koyama: "L-Glutamate-induced swelling of cultured astrocytes" Adv.Exp.Med.Biol.315. 375-380 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Koyama: "Endothelins modulate dibutyryl cAMP-induced stellation of cultured astrocytes" Brain Res.600. 81-88 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Matsuda: "Selective induction of α1 isoform of (Na-K)ATPase by insulin/insulin like growth factor-1 in cultured astrocytes" Arch.Biochem.Biophyis.307. 175-182 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Koyama: "L-Glutamate-stimulated taurine release from rat cultured astrocytes" J.Neurosci.Res.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Koyama: "Endothelins are extracellular signals modulating cytoskeletal actin organization in rat cultured asorocytes" Neuroscience. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Koyama: "L-Glutamate-induced swelling of cultured astrocytes" Adv.Exp.Med.Biol.315. 375-380 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Koyama: "Endothelins modulate dibutyryl cAMP-induced stellation of cultured astrocytes" Brain Res.600. 81-88 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Matsuda: "Selective induction of al isoform of (Na-K)ATPase by insulin/insulin-like growth factor-1 in cultured astrocytes" Arch.Biochem.Biophys.307. 175-182 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Koyama: "L-Glutamate-stimulated taurine release from rat cerebral cultured astrocytes" J.Neurosci.Res.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Koyama: "Endothelins are extracellular signals modulating cytoskeletal actin organization in cultured astrocytes" Neuroscience. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Koyama: "Endothelins modulate dibutyryl cAMP-induced stellation of cultured astrocytes" Brain Res.600. 81-88 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Matsuda: "Selective induction of α1 isoform of (Na-K)-ATPase by insulin insulin-like growth factor-1 in cultured astrocytes" Arch.Biochem.Biophys.307. 175-182 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Koyama: "Endothelins are extracellular signals modulating astrocytic F-actin in cultured astrocytes" Neuroscience. (in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Koyama: "L-Glutamate-stimulated taurine release from rat cerebral cultured astrocytes" J.Neurosci.Res.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Koyama,T.Ishibashi & A.Baba: "L-Glutamate-induced swelling of cultured astrocytes" Adv.Exp.Med.Biol.315. 375-380 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Koyama,T.Ishibashi,K.Hayata & A.Baba: "Endothelins modulate dibutyryl,cAMP-induced stellation of cultured astrocytes" Brain Res.600. 81-88 (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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