| Project/Area Number |
04557126
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | NIIGATA UNIVERSITY |
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Principal Investigator |
ONO Teruo NIIGATA UNIVERSITY SCHOOL OF MEDICINE PROFESSOR, 医学部, 教授 (00000927)
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Masahiko NIIGATA UNIVERSITY SCHOOL OF MEDICINE PROFESSOR, 医学部, 教授 (30018915)
HITOMI Masahiro NIIGATA UNIVERSITY SCHOOL OF MEDICINE RESEARCH ASSOCIAT, 医学部, 助手 (40218730)
SAKAKIBARA Jun NIIGATA UNIVERSITY SCHOOL OF MEDICINE RESEARCH ASSOCIAT, 医学部, 助手 (90242403)
FUJII Hiroshi NIIGATA UNIVERSITY SCHOOL OF MEDICINE ASSOCIATE PROFESS, 医学部, 助教授 (90165340)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1993: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1992: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | Fatty Acid-Binding Protein / H-FABP / I-FABP / I-15P / Myocardial Infarct / Serum Mark / L-FABP |
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| Research Abstract |
We have succeeded in cloning of two FABP family new members. One is a I-15P from intestine and the other is c-FABP from rat skin. The deduced I-15P cDNA sequence of 127 amino acids was identical that of rat I-15P protein purified from rat intestinal epithelium. Northern blot analysis indicated that the same was deteceted in the ovary suggesting that I-15P or a homologous protein might in volved in the metabolism of steroids in steroid hormone-producing tissues. Another new family member, c-FABP exhibits 50 % identity to myelin P2 protein, adipocyte FABP and heat FABP.
We have developed the assay methods of serum FABPs and tested whether serum FABP is available as a marker of damaged tissues. Serum I-FABP was measured in rats with intestinal ischemic condition. The I-FABP level increased rapidly at 1, 2, 4 and 8 hr after ligation of rat intestine. It also increased at 1 hr after a 30-min transient occlusion and then returned to a normal level. Histological studies supported I-FABP release from necrotic adsorptive epithelial cell. The results suggest that the I-FABP is a useful biochemical maker for intestinal ischemia, particularly in the early reversible phase. The serum H-FABP determinations performed on 9 patitents with acute myocardial infarct (AMI) demonstrated that 4 serum samples obtaining within 1.5 h after heart stroke were within normal level, whereas 5 serum samples obtained with more than 1.5 h after heart stroke were showed elevated H-FABP levels. However, it reached maximum levels faster than serum creatine kinase MB(CK-MB) and creatine phosphokinase (CKB) levels within 6 h after percutaneous transluminal coronary angioplasty (PTCA) treatment. The increase of urinary H-FABP after PTCA treatment is in a marked contrast to urinary CK-MB and CPK which has been scarcely detected. Thus, serum H-FABP is a useful biochemical marker for estimation of recirculation by PTCA treatment rather than the onset of heart stroke.
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