| Project/Area Number |
04557127
|
|---|
| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | Gifu University |
|---|
Principal Investigator |
NOMA Akio School of Medicine, Gifu Univ. Professor, 医学部, 教授 (30208384)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YOSHIMURA Yoshimichi Tokuyama Soda Co., Ltd., Tsukuba Res.Lab. Senior Researcher, つくば研究所, 主任研究員
TSUKADA Yutaka SRL, Inc., Hachioji Laboratories Director, 研究部, 部長
MAKINO Kazuhiko School of Medicine, Gufi, Univ. Assistant, 医学部, 助手 (80181618)
SEISHIMA Mitsuru School of Medicine, Gifu Univ. Assistant, 医学部, 助手 (10171315)
ABE Akira University Hospital, Gifu Univ. Lecturer, 医学部附属病院, 講師 (30175898)
|
|---|
| Project Period (FY) |
1992 – 1993
|
| Project Status |
Completed (Fiscal Year 1993)
|
| Budget Amount *help |
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 1993: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1992: ¥10,100,000 (Direct Cost: ¥10,100,000)
|
|---|
| Keywords | Apolipoprotein(a) / ELISA / TIA / LIA / Apo(a) isoform / Phenotype / Genotype / Restriction enzyme KpnI / 免疫比濁法 / ラテックス免疫凝集比濁法 / 自動分析装置 / パーソナルコンピューター / 表現型 / 遺伝型 / ラテックス免疫比濁法 / 自動化 / Lp(a)表現型 / 虚血性心疾患 / コンピュータ処理 |
|---|
| Research Abstract |
Although several different techniques to determine serum lipoprotein(a) (Lp(a)) were developed, many laboratories still lack reliable any easy-to-perform assay for Lp(a) measurements. At present, ELISA method is the most commonly used technique to measure serum Lp(a), because it is highly sensitive, specific in principle, and does not require radio-isotopes. Gowever, the ELISA method requires highly diluted serum as samples, measurement in duplicate to achieve satisfactory precision, and several hours for the assay. The immunonephelometric and immunoturbidimetric assays are suitable for the clinical laboratories, in that they are rapid and easy-to-perform, have a high throughput, and can be automated. The turbidimetric immunoassay (TIA) and latex-enhanced immunoassay (LIA) methods, however, also are sensitive to differences in size of the analyte being measured. After the careful evaluations, we have chosen automated procedure for Lp(a) measurement by an LIA method having satisfactory precision, specificity and sensitivity.
On the other hand, a new system has been employed to determine the apo(a) phenotypes. After electrophoresis and immunoblotting, Rf values of apo(a) isoform bands were measured using a computerized digital micro scale and apo(a) phenotypes were categorized automatically based on the Rf values. Recently, in an attempt to classify the apo(a) genotypes, we have investigated the basis of the apo(a) size polymorphism by pulsed-field gel electrophoresis of genomic DNA employing the restriction enzymeKpnI and an apo(a) kringle IV specific probe.
|
