| Project/Area Number |
04558022
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | Osaka University |
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Principal Investigator |
SHIMONISHI Yasutsugu Inst.for Protein Res., Osaka Univ., Professor, 蛋白質研究所, 教授 (00029951)
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| Co-Investigator(Kenkyū-buntansha) |
KAMMEI Yoshihiro MS group, JEOL Ltd., Group leader, 質量分析開発グループ, グループ長
TAKAO Toshifumi Inst.for Protein Res., Osaka Univ., Instructor, 蛋白質研究所, 助手 (10197048)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥10,000,000 (Direct Cost: ¥10,000,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1992: ¥8,600,000 (Direct Cost: ¥8,600,000)
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| Keywords | Pulsed FAB / Array detector / Four-sector tandem mass spectrometer / Function generator / Initial ionization event / Sample lifetime / MS / MS sequencing / Femtomole / パルスイオン化 / 高感度質量分析 / MS / MSスペクトル / 蛋白質構造解析 / 質量分析 / アレイ検出器 / 高感度測定 |
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| Research Abstract |
A pulsed FAB has been proposed to be a powerful technique for dramatic enhancement in sensitivity and increase in sample lifetimes. Here we developed a new pulsed FAB control system coupled with a photodiode-based array detector on a four-sector tenden mass spectrometer.
The trigger pulse initially generated from the array detector control system is transmitted to the data acquisition processor via an optical fiber line, and them, to a function generator. The function generator is designed to provide a set of pulses of various repetitive frequencies and duty cycles. A high voltage power supply combined with the function generator allows a primary ion beam to be accelerated at up to a 6-kV potential with a quick rising and falling time less than 1 millisecond. The pulsing of primary ion beam is synchronized with start-up of the data acquisition on the array detector.
When the system was tested on the second MS using a pulsed Xe atom beam, the protonated molecular ion peaks of peptides were obtained with 10 times higher intensity than those by a continuous FAB in the initial ionization event. The detection limits for angiotensin I (m/z 1296.7) and substance P (1347.7) were about 10 femtomoles. This new pulsed FAB control system was further demonstrated to be effective for reducing sample amounts required for MS/MS sequencing of peptides to the subfemtomole order.
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