| Project/Area Number |
04640343
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
固体物性
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
SATO Kiyotaka Faculty of Applied biological Science Hiroshima University Professor, 生物生産学部, 教授 (80034479)
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| Co-Investigator(Kenkyū-buntansha) |
UENO Satoru Faculty of Applied biological Science Hiroshima University Assistant Professor, 生物生産学部, 講師 (50243605)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | light scattering tomography / surface microtopography / Hen-egg white lysozyme crystal / etch pit / lattice defect / crystal growth / point defect / line defect / 表面マイクロトポグラフ法 |
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| Research Abstract |
Laser scattering tomography (LST) has been applied to observe lattice defects in protein crystals for the first time. Hen-egg white lysozyme crystal of orthorhombic modification was grown by a batch method using heterogeneous crystallization method. The crystal sized employed were 1-2 mm in thickness and 5-7 mm in length. The following conclusions were obtained : (1) LST figures were observed from the crystal portions where no defect was detectable with optical microscopy. (2) The polarization dependence proved that both bulk-type and point defect-type defects are present. The bulk-type LST patterns were often parallel to the crystal edges, and assumed to correspond to inclusions. (3) The polarization dependence could differentiate the point-defect type and bulk defect type. From these results, we conclude that LST is effective to macroscopically characterize the protein crystals.
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