Inprovement of Thioester Method and Synthesis of Cysteine-Containing Proteins
| Project/Area Number |
04640527
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
天然物有機化学
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| Research Institution | Osaka University |
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Principal Investigator |
AIMOTO Saburo Osaka University, Institute for Protein Research, Professer, たんぱく質研究所, 教授 (80029967)
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| Co-Investigator(Kenkyū-buntansha) |
HOJO Hironobu Osaka University, Institute for Protein Research, Instructor, たんぱく質研究所, 助手 (00209214)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1992: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Peptide syntheis / Protein synthsis / thioester method / Segnent codensation / Solid-phase method / TAT protein / ペプチド合成法 / 蛋白質合成法 / タンパク質合成 / チオエステル / 固相合成法 |
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| Research Abstract |
In order to expand the thioester method, we searched the route for the preparation of S-proted two methods for the preparation of S-protected peptide thioesters via low- or high-HF treatment of protected peptide resins obtained by Boc strategy of a solid-phase method. This year, a more efficient method for the preparation of S-protected peptide thioester wwas developed. In this method, peptide chain was elongated by using Npys-amino acids. A cysteine residue is introduced by Npys-Cys(Tmb) (Tmb : 2,4,6-trimethylbenzyl). Aprotected peptide resin was treated by reagent K to give an S-protected peptide thioester. The Tmb group on the mercapt group was stable during reagent K treatment and segment condensation.
Vased on this preparation strategy, cysteine-containing peptide thioesters were synthesized and used for the syntheses of the barmase-like domain ( 112 amino acid residues) in DNA-directed RNA polymerase II of Saccharomyces cerevisiae (RPSc(299-410)). RPSc(299-410) was successfully synthesided. This strateby was also applied to the preparation of tAT protein of HIV-1, which has 7 cysteine residues, was also synthesized by the thioester method. the interaction between TAT protein and zinc ions is now under investigation.
To conclude, the thioester method can be applied to the synthsis of proteins with cysteine residues as sell as without them.
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Report
(3 results)
Research Products
(12 results)
