| Project/Area Number |
04640592
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
遺伝学
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| Research Institution | Saitama University |
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Principal Investigator |
INOUE Hirokazu Saitama University, Faculty of Science, Associate Professor, 理学部, 助教授 (60114203)
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| Co-Investigator(Kenkyū-buntansha) |
ISHII Chizu Saitama University, Faculty of Science, Assistant Professor, 理学部, 助手 (00114215)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | homologous recombination / Neurospora crassa / non-homologous recombination / cloning / mei-3 / RAD51 / DNA repair / recombinational repair / 相同的組み換え / 非相同的組み換え / DNA修復欠損株 |
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| Research Abstract |
About 40 different Neurospora DNA repair mutants were characterized genetically and their sensitivities to mutagens were extensively tested. From those results, we isolated 5 to 6 as candidates of recombinational repair-deficient mutants and tested their abilities of integration of a plasmid pMTR to genome DNA.Mutants, mei-3, mei-2, uvs-6, mus-25 showed very low homologous recombination frequencies. We tried to clone those genes using sib selection method. Cloned mei-3 showed that it has high similarity to E.coli recA and S.cerevisiae RAd51. Gene mus-25 was a homolog of S.cerevisiae RAD54. They are member of recombinational repair in yeast. Therefore, it seems that this group including mei-3 and mus-25, functions in homologous recombination system in Neurospora crassa.
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