| Project/Area Number |
04640650
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
動物発生・生理学
|
|---|
| Research Institution | TOHOKU UNIVERSITY |
|---|
Principal Investigator |
IDE Hiroyuki Tohoku Univ., Dept. of Biol., Prof., 理学部, 教授 (70022704)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OHSUGI Kojune Tohoku Univ., Dept. of Biol., 理学部, 助手 (00201377)
|
|---|
| Project Period (FY) |
1992 – 1993
|
| Project Status |
Completed (Fiscal Year 1993)
|
| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
|---|
| Keywords | Retinoic Acid / Limb bud / Regeneration / Pettern formation / 細胞親和性 |
|---|
| Research Abstract |
To examine common mechanisms of Retinoic acid (RA)-induced pattern formation in the developing limb bud and regenerating limb blastema, chick limb bud at satge 20 was treated with RA for 10 hours by implanting RA-bead and then the mesenchymal tissue near the bead was transplanted to the anterior margin of otherlimb bud at stage 18-19. The RA-treated and transplanted tissues formed all the cartilage elements from humerus to degits, although the control transplants formed only digits according to their fate. This "proximalization" occurred independently of posteriorization, that is, induction of ZPA.The expression pattern of HoxA, which was known to change corresponding to the proximodistal positional value in normal limb development, changed to show the proximalization in RA-treated transplants. The cell affinity also changes according to the positional values. The dissociated cells of RA-treated mesenchyme showed the proximalization to segregate from those of untreated tissues in monolayr culture. These results suggest that the cells of RA-treated mesenchyme of developing limb changes their positional value to show proximalization other than posteriorization as shown previously in regeneration blastema of salamander limb.
|
