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Molecular basis of neuronal plasticity

Research Project

Project/Area Number 04640692
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物形態・分類学
Research InstitutionJuntendo University School of Medicine

Principal Investigator

MATSUMOTO Akira  Juntendo Univ., Sch. Med., Assoc. Prof., 医学部, 助教授 (80053263)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1992: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsbeta-actin / beta-tubulin / mRNA / in situ hydridization / androgen / rat / lumber spinal cord / motoneuron / beta-アクチン / beta-チューブリン
Research Abstract

Motoneurons of the spinal nucleus of the bulbocavornosus (SNB) of male rats innervate the perineal striated muscles bulbocavernosus and lavator ani that attach to the penis. They have an important role in copulatory behavior, which is sensitive to alterations in circulating levels of androgen. Androgen is also known to play a crucial role in reorganizing the neuronal elements and synaptic connections in androgen-sensitive SNB motoneurons. To clarify molecular mechanisms of androgen for neuronal plasticity, we examined androgenic regulation of the mRNA expression of cytoskeletal proteins such as beta-actin and beta-tubulin in the SNB motoneurons of adult male rats. Adult male rats were castrated and implanted with silastic tubes containing testosterone or nothing. Animals were sacrificed 4 weeks later. Chick beta-actine and mouse beta-tubulin complementary CNAs were used to detect mRNAs encoding beta-actin and beta-tubulin of the SNB motoneurons, respectively. The radiolabeled DNA probes were applied o in situ hybridization histochemistry. Motoneurons in the retrodorsolateral nucleus (RDLN), which innervate hindlimb musculature, were also examined. Autoradiographic signals for beta-actin and beta-tubulin mRNAs were found to be localized on the somata and proximal dendrites of SNB and RDLN motoneurons. Removal of androgen by castration significantly reduced the expression level of these mRNAs in the SNB motoneurons, whereas the change was prevented by testosterone treatment. ON the contrary, castration or testosterone treatment did not induce any changes in the expression level of these mRNAS in androgen-insensitive RDLN motoneurons. These results suggest that androgen regulates the expression of cytoskeletal protein genes in the SNB motoneurons and may provide evidence for the molecular mechanisms of hormonally-induced neuronal plasticity in the SNB motoneuron.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Matsumoto,A.et al.: "Effect of androgen on the expression of gap junction and β-actin mRNAs in adult rat motoneurons." Neurosci.Res.14. 133-144 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto,A.: "Sex steroid induction of synaptic reorganization in adult neuroen-docrine brain." Rev.Neurosci.3. 287-306 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto,A.: "Hormonally induced synaptic plasticity in the adult neuroendocrine brain." Zool.Sci.9. 679-695 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 松本明: "ヒトはどうして同性愛となるのか---その生物学的考察" 科学朝日. 52. 118-123 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto,A.et al.: "Androgenic regulation of expression of β-tubulin mRNA in motoneurons of the spinal nucleus of the bulbocavernosus." J.Neuroendocrinol.5. 357-363 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto,A.and Ishii,S.: "Atlas of Endocrine Organs" Springer-Verlag, 307 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto, A., Arai, Y.Urano, A.and Hyodo, S.: "Androgen regulates beta-actin mRNA expression in the motoneurons of lumbar spinal cords in adult male rats" Zool. Sci.9. 1267 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto, A., Arai, Y.Urano, A.and Hyodo, S.: "Effecgt of androgen on beta-actin mRNA expression in the motoneruons of lumber spinal cords in adult male rats" Neurosci. Res.Suppl. 17. S301 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto, A., Arai, Y.: "Aypothalamus" "Atlas of Endocrine Organs" (Matsumoto, A.and Ishii, S.eds.) Springer-Verlag. 25-38 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto, A., Arai, Y.Urano, A.and Hyodo, S.: "Effect of androgen on the expression of gap junction and beta-actin mRNA in adult rat motoneurons" Neurosci. Res.14. 133-144 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto, A.: "Sex steroid induction of synaptic reorganization in adult neuroendocrine brain" Rev. Neurosci.3. 287-306 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto, A.: "Hormonally induced synaptic plasticity in the adult neuroendocrine brain" Zool. Sci.9. 679-695 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto, A., Arai, Y.and Hyodo, S.: "Androgenic regulation of expression of beta-tubulin messenger ribonucleic acid in motoneurons of the spinal nucleus of the bulbocavernosus" J.Neuroendocrinol.5. 5357-363 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto, A., Arai, Y.and Hyodo, S.: "Androgenic regulation of beta-tubulin mRNA expression in the motoneurons of lumber spinal cords in adult male rats" Zool. Sci.Suppl. 10. 126 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto, A., Arai, Y.and Hyodo, S.: "Androgen regulates beta-tubulin expression in the motoneurons of lumber spinal cords in adult male rats" Sci. Neurosci. Abstr.19. 883 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto,A.et.al.: "Androgenic regulation of expression of beta-tubulin mRNA in motoneurons of the spinal nucleus of the bulbocavernosus." J.Neuroendocrinol.5. 357-363 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Matsumoto,A.et al.: "Effect of androgen on the expression of gap junction and β-actin mRNAs in adult rat motoneurons." Neuroscience Research. 14. 133-144 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Matsumoto,A.: "Hormonally induced synaptic plasticity in the adult neuroendocrine brain." Zoological Science. 9. 679-695 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Matsumoto,A.: "Sex steroid induction of synaptic reorganization in adult neuroendocrine brain" Reviews in the Neurosciences. 3. 287-306 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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