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Studies on the enzymatic characteristics of methar monooxygenase and its trichloroethylene degrading activity

Research Project

Project/Area Number 04650497
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 都市工学・衛生工学
Research InstitutionOsaka University

Principal Investigator

FURUKAWA Kenji  Osaka Univ., Environmental Eng., Assoc.Prof., 工学部・環境工学科, 助教授 (60029296)

Co-Investigator(Kenkyū-buntansha) IKE Michihiko  Osaka Univ., Environmental Eng., Res.Assoc., 工学部・環境工学科, 助手 (40222856)
IWAHORI Keisuke  Osaka Univ., Environmental Eng., Res.Assoc,, 工学部・環境工学科, 助手 (40183199)
FUJITA Masanori  Osaka Univ., Environmental Eng., Professor, 工学部・環境工学科, 教授 (70029289)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsmethane utilizing bacteria / acclimated bacteria / trichloroethylene / methanemonooxygenase / bio-remediation / ground water pollution / chloroform / solubleメタンモノオキシゲナーゼ / particulateメタンモノオキシゲナーゼ / Cu^<2+> / 有機塩素系溶媒 / メタン資化性菌 / 揮発性塩素化脂肪族炭化水素
Research Abstract

We have showed that the methane utilizing bacteria widely inhabit the natural water and soil environments. There existed big differences in the trichloroethylene degrading activities of our prepared methane utilizing enrichment cultures. Among these enrichment cultures, the enrichment culture prepared from the ground water polluted with trichloroethylene showed the highest trichloroethylene degrading activity. Effective trichloroethylene degradation was obtained under the cultural condition with low methane partial pressure. Treatment temperature gave much influence on its degrading activity. Enrichment culture cultivated on methanolic medium showed a low trichloroethylene degrading activity. The responsible enzyme for trichloroethylene degradation was proved to be a methane monooxygenase from the inhibition experiments using acetylene. The enrichment culture degraded 30 mg/L of trichloroethylene effectively. The enrichment culture could not degrade tetrachloroethylene and 1,1,1-trichloroethane, but could degrade chloroform. Trichloroethylene degrading activity of the enrichment culture decreased during the repeated contacts with trichloroethylene.
Isolation of methane utilizing bacterium which can degrade trichloroethylene was failed owing to its strict growth condition.
Methane monooxygenase belonging to the enrichment culture was studied enzymatically. Methane monooxygenase activity was detected in the soluble (cytoplasmic) fraction, but was not detected in particulate (membrane) fraction.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 古川憲治、田波重信 藤田正憲: "メタン資化性細菌によるトリクロロエチレンの分解" 日本生物工学会大会講演集. 167 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kenji Furukawa, Shigenobu Tanami and Masanori Fujita: "Trichloroethylene degradation by methane utilizing bacteria" Proceedings of Annual Meeting of the Society of Fermentation and Bioengineering, Japan. 167 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 古川憲治,田波重信,藤田正憲: "メタン資化性細菌によるトリクロロエチレンの分解" 地下水汚染とその防止対策に関する研究集会講演集. 2. 24 (1992)

    • Related Report
      1993 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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