Preparation of Hydrolase Immobilized on a Reversibly Soluble-Insoluble Polymer and Its Application to Production of Biomass Fuels.
| Project/Area Number |
04650869
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
反応工学
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| Research Institution | Niigata University |
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Principal Investigator |
TANIGUSHI Masayuki Niigata Univ. Faculty of Eng. Associate Professor, 工学部, 助教授 (00163634)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Hydrolase / Immobilized Enzyme / Biomass Fuels / Starch / Cellulose / セルロース / 刺激応答性高分子 / キシラン |
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| Research Abstract |
Preparation of a responsive-hydrolase :
A copolymer of methacrylic acid (MAA) and N-isopropyl acrylamide (NIPAM) was used as a novel, reversibly soluble-insoluble support whose solubility changes depending on the temperature of the solution. Amylase (Dabiase K-27) immobilized covalently on the thermo-responsive polymer showed good solubility response : The immobilized enzyme (D-MN) was in a soluble state below 32ﾟC, but insoluble above 42ﾟC.
Properties of a responsive amylase : D-MN in a soluble state has a high specific activity for the hydrolysis of soluble or uncooked starches. The solubility response of D-MN to changes in the temperature of the solution was more sensitive when 0.5% NaCl was added to a buffer solution (pH 4.5) with D-MN than in the buffer solution without NaCl.
Repeated use of a responsive hydrolase :
D-MN was used successively for repeated hydrolysis reactions of soluble and uncooked starches, in which D-MN was insolubilized either by changing the temperature of the reaction mixture from 30ﾟC to 36ﾟC with 0.5% NaCl or by adjusting the NaCl concentration of the reaction mixture from 0% to 1% at 30ﾟC.
Repeated hydrolysis reaction of biomass :
In the repeated hydrolysis, glucose was produced successively from the soluble and uncooked starches, and D-MN could be repeatedly used after being recovered from the reaction product by centrifugation at the end of each batchwise hydrolysis.
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Report
(3 results)
Research Products
(7 results)
