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Mechanism of ethylene formation by the EFE of a mold and a bacterium

Research Project

Project/Area Number 04650879
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 発酵工学
Research InstitutionKumamoto Institute of Technology

Principal Investigator

OGAWA Takahira  Kumamoto Institute of Technology, Dep.Applied Microbial Technol., Professor, 工学部, 教授 (40029244)

Co-Investigator(Kenkyū-buntansha) FUJII Takao  Kumamoto Institute of Technology, Dep.Applied Microbial Technol., Associate Prof, 工学部, 助教授 (80165331)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsEthylene / Ethylene-forming enzyme / 2-Oxoglutarate / Pseudomonas syringae / Penicillium digitatum / α-ケトグルタール酸 / L-アルギニン
Research Abstract

A single enzyme isolated from Pseudomonas syringae pv.phaseolicola PK2 simultaneously catalyzed two reactions, namely, the formation of ethylene and succinate from 2-oxoglutarate, at a molar ratio of 2 : 1. In the main reaction, 2-oxoglutarate was dioxygenated to produce one molecule of ethylene and three molecules of carbon dioxide. In the sub-reaction, both 2-oxoglutarate and L-arginine were mono-oxygenated to yield succinate plus carbon dioxide and L-hydroxyarginine, respectively, the latter being further transformed to guanidine and L-DELTA^1-pyrroline-5-carboxylate. We propose a dual-circuit mechanism for the entire reaction, in which the binding of L-arginine and 2-oxoglutarate in a Schiff-base structure generates a common intermediate for two reactions.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] Hideo Fukuda: "Two reactions are simultaneously catalyzed by a single enzyme:The arginine-dependent simultaneous formation of two products." Biochemical and Biophysical Research Communications. 188. 483-489 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] HIDEO FUKUDA, TAKAHIRA OGAWA, MASATO TAZAKI, KAZUHIRO NAGAHAMA, TAKAO FUJII, SUMIO TANASE AND YOSHIMASA MORINO: "Two reactions are simultaneously catalyzed by a single enzyme : The arginine-dependent simultaneous from 2-oxoglutarate by an enzyme from Pseudomonas syringae" Biochemical and Biophysical Research Communications. Vol.188. 483-489 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hideo Fukuda: "Two reactions are simultaneously catalyged by a single enryme:The arginine-dependent simultaneous formation two products." Biochemical and Biophysical Research Communicatins. 188. 483-489 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] KAZUHIRO NAGAHAMA: "Purification and properties of an ethylene-forming enuyme from pseudomonas syringae pv. phaseolicola PK2" Journal of General Microbiology. 137. 2281-2286 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] HIDEO FUKUDA: "Two reactions are simultaneously catalyzed by a single enuyme : The arginine-dependent simultaneous formation of two products,ethylene and succinate,from 2-oxoglutarate by an enuyme from Pseucdomonas syringae" Biochemical and Biophysical Research Communications. 188. 483-489 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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